Recombinant Human Cdc42 effector protein 2 (CDC42EP2)

Code CSB-YP005013HU
MSDS
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Source Yeast
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Code CSB-EP005013HU
MSDS
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Source E.coli
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Code CSB-EP005013HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP005013HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP005013HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
CDC42EP2
Uniprot No.
Alternative Names
Binder of Rho GTPases 1; BORG1; BORG1_HUMAN; CDC42 effector protein (Rho GTPase binding) 2; Cdc42 effector protein 2; Cdc42ep2; CEP2; CRIB-containing BOGR1 protein
Species
Homo sapiens (Human)
Expression Region
2-210
Target Protein Sequence
STKVPIYLK RGSRKGKKEK LRDLLSSDMI SPPLGDFRHT IHIGSGGGSD MFGDISFLQG KFHLLPGTMV EGPEEDGTFD LPFQFTRTAT VCGRELPDGP SPLLKNAISL PVIGGPQALT LPTAQAPPKP PRLHLETPQP SPQEGGSVDI WRIPETGSPN SGLTPESGAE EPFLSNASSL LSLHVDLGPS ILDDVLQIMD QDLDSMQIPT
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Probably involved in the organization of the actin cytoskeleton. May act downstream of CDC42 to induce actin filament assembly leading to cell shape changes. Induces pseudopodia formation in fibroblasts in a CDC42-dependent manner.
Gene References into Functions
  1. Studies indicate some of the functional and mechanistic roles of the binder of Rho GTPases Borg1-5 proteins (Cdc42EP1-5), including cytoskeletal remodelling and signalling. PMID: 27913681
  2. CDC42EP2 is upregulated in human PDLCs subjected to tensile stress related to mechano-induced cell cycle arrest. PMID: 20934684
  3. activations of Rac1 and Cdc42 are involved in the hypoxia-induced production of angiogenesis-promoting factors and tumor suppressors, and suggest that the Rho family GTPases Rac1 and Cdc42 may contribute to the hypoxia-mediated angiogenesis. PMID: 16395716
Subcellular Location
Endomembrane system; Peripheral membrane protein. Cytoplasm, cytoskeleton.
Protein Families
BORG/CEP family
Tissue Specificity
Highly expressed in the heart. Weakly expressed in the pancreas and liver.
Database Links

HGNC: 16263

OMIM: 606132

KEGG: hsa:10435

STRING: 9606.ENSP00000279249

UniGene: Hs.343380

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