Recombinant Human Chitinase domain-containing protein 1(CHID1)

In Stock
Code CSB-EP883614HU
Size US$2466
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details

Purity Greater than 90% as determined by SDS-PAGE.
Target Names CHID1
Uniprot No. Q9BWS9
Research Area Tags & Cell Markers
Alternative Names CHID1; CHID1_HUMAN; Chitinase domain containing 1; Chitinase domain-containing protein 1; FLJ42707; GL008; MGC3234; PSEC0104; SB139; SI-CLP; SICLP; Stabilin 1 interacting chitinase like protein; Stabilin-1-interacting chitinase-like protein
Species Homo sapiens (Human)
Source E.coli
Expression Region 20-393aa
Target Protein Sequence TLSKSDAKKAASKTLLEKSQFSDKPVQDRGLVVTDLKAESVVLEHRSYCSAKARDRHFAGDVLGYVTPWNSHGYDVTKVFGSKFTQISPVWLQLKRRGREMFEVTGLHDVDQGWMRAVRKHAKGLHIVPRLLFEDWTYDDFRNVLDSEDEIEELSKTVVQVAKNQHFDGFVVEVWNQLLSQKRVGLIHMLTHLAEALHQARLLALLVIPPAITPGTDQLGMFTHKEFEQLAPVLDGFSLMTYDYSTAHQPGPNAPLSWVRACVQVLDPKSKWRSKILLGLNFYGMDYATSKDAREPVVGARYIQTLKDHRPRMVWDSQASEHFFEYKKSRSGRHVVFYPTLKSLQVRLELARELGVGVSIWELGQGLDYFYDLL
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 46.8kDa
Protein Length Full Length of Mature Protein
Tag Info N-terminal 6xHis-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Citations

SI-CLP inhibits the growth of mouse mammary adenocarcinoma by preventing recruitment of tumor-associated macrophages. Yin S,International journal of cancer,2019

Sample type: Primary monocytes
Sample species: Mouse
Application: Cell migration
Review: BMDM were generated in Balb/c mice (n=5) and incubated for 30 min with 100 ng/ml of purified murine SI-CLP. BMDM (5x10 5 ) were allowed to migrate towards 100 ng/ml of murine CCL2 in RPMI medium for 3h. The chemotactic index was calculated by dividing the number of migrated cells in SI-CLP group by the number of migrated cells in control group (without SI-CLP). The experiment was performed in triplicates and data are presented as mean ± SD; unpaired t-test was used for statistical analysis.
PMID: 31525266

Target Data

Function Saccharide- and LPS-binding protein with possible roles in pathogen sensing and endotoxin neutralization. Ligand-binding specificity relates to the length of the oligosaccharides, with preference for chitotetraose (in vitro).
Gene References into Functions
  1. This review comprehensively analyzes recent data about expression pattern, and involvement of human YKL-40, YKL-39 and SI-CLP in cancer. [review] PMID: 26733160
  2. stabilin-1-interacting chitinase-like protein functions as a regulator of the inflammatory response in rheumatoid arthritis by macrophages. PMID: 24470346
  3. Structure of human stabilin-1 interacting chitinase-like protein (SI-CLP) reveals a saccharide-binding cleft with lower sugar-binding selectivity. PMID: 20724479
  4. The crystals of SI-CLP belong to the space group P3(2)21 with unit cell parameters a=b=99.79 A, c=250.53 A, alpha=beta=90 degrees, gamma=120 degrees. PMID: 19275751
Subcellular Location Secreted, Lysosome
Protein Families Glycosyl hydrolase 18 family
Tissue Specificity Expressed in cells of monocytic, T, B and epithelial origin.
Database Links

HGNC: 28474

OMIM: 615692

KEGG: hsa:66005

UniGene: Hs.144468

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