Recombinant Human Coagulation factor XIII A chain(F13A1)

Code CSB-YP007919HU
Size US$1916
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details

Purity Greater than 90% as determined by SDS-PAGE.
Target Names F13A1
Uniprot No. P00488
Research Area Cardiovascular
Alternative Names bA525O21.1 (coagulation factor XIII; A1 polypeptide); Coagulation factor XIII A chain; Coagulation factor XIII A1 polypeptide ; Coagulation factor XIII A1 subunit; Coagulation factor XIII; A polypeptide; Coagulation factor XIIIa; F13A; F13A_HUMAN; F13a1; Factor XIIIA; Fibrin stabilizing factor; A subunit; Fibrinoligase; FSF; A subunit; Protein glutamine gamma glutamyltransferase A chain ; Protein-glutamine gamma-glutamyltransferase A chain; TGase ; Transglutaminase A chain; Transglutaminase; plasma; Transglutaminase. plasma
Species Homo sapiens (Human)
Source Yeast
Expression Region 39-732aa
Target Protein Sequence GVNLQEFLNVTSVHLFKERWDTNKVDHHTDKYENNKLIVRRGQSFYVQIDFSRPYDPRRDLFRVEYVIGRYPQENKGTYIPVPIVSELQSGKWGAKIVMREDRSVRLSIQSSPKCIVGKFRMYVAVWTPYGVLRTSRNPETDTYILFNPWCEDDAVYLDNEKEREEYVLNDIGVIFYGEVNDIKTRSWSYGQFEDGILDTCLYVMDRAQMDLSGRGNPIKVSRVGSAMVNAKDDEGVLVGSWDNIYAYGVPPSAWTGSVDILLEYRSSENPVRYGQCWVFAGVFNTFLRCLGIPARIVTNYFSAHDNDANLQMDIFLEEDGNVNSKLTKDSVWNYHCWNEAWMTRPDLPVGFGGWQAVDSTPQENSDGMYRCGPASVQAIKHGHVCFQFDAPFVFAEVNSDLIYITAKKDGTHVVENVDATHIGKLIVTKQIGGDGMMDITDTYKFQEGQEEERLALETALMYGAKKPLNTEGVMKSRSNVDMDFEVENAVLGKDFKLSITFRNNSHNRYTITAYLSANITFYTGVPKAEFKKETFDVTLEPLSFKKEAVLIQAGEYMGQLLEQASLHFFVTARINETRDVLAKQKSTVLTIPEIIIKVRGTQVVGSDMTVTVQFTNPLKETLRNVWVHLDGPGVTRPMKKMFREIRPNSTVQWEEVCRPWVSGHRKLIASMSSDSLRHVYGELDVQIQRRPSM
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 81.2kDa
Protein Length Full Length of Mature Protein
Tag Info N-terminal 6xHis-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

Target Data

Function Factor XIII is activated by thrombin and calcium ion to a transglutaminase that catalyzes the formation of gamma-glutamyl-epsilon-lysine cross-links between fibrin chains, thus stabilizing the fibrin clot. Also cross-link alpha-2-plasmin inhibitor, or fibronectin, to the alpha chains of fibrin.
Gene References into Functions
  1. Overall, genetically-determined FXIIIA levels have a significant long-term prognostic role, suggesting that a pharmacogenetics approach might help to select those AMI patients at risk of poor prognosis in the need of dedicated treatments. PMID: 30223472
  2. Inflammatory monocytes highly express Factor XIIIA, which promotes fibrin cross-linking to create a scaffold for lung squamous carcinomas cell invasion and metastases. PMID: 29777108
  3. Effect of factor XIII levels and polymorphisms on the risk of myocardial infarction in young patients PMID: 29484525
  4. Coagulation factor FXIII-A (FXIIIA) was found to express specifically in the fetal beta islets but not in the alpha/delta islets. PMID: 29424810
  5. Factor XIIIa (AC-1A1) is a sensitive and specific nuclear marker for sebaceous differentiation...which can be utilized to aid in the diagnosis of sebaceous neoplasms. PMID: 28873247
  6. Our meta-analysis supports an association between F13A1 Val34Leu and recurrent pregnancy loss. PMID: 28683377
  7. A brief history of some key events in the conversion of fibrinogen to fibrin has been reviewed. (Review) PMID: 27519977
  8. The results of this study suggest that these biomarker F13A1 can serve as a potential non-invasive early diagnosis platform reflecting PiB-PET imaging for Mild Cognitive Impairment and Alzheimer's Disease. PMID: 27392853
  9. F13A1 gene mutations in 73 patients treated with recombinant FXIII-A2 PMID: 28520207
  10. Results show that the missense mutations-causing mild FXIII de fi ciency influence different aspects of FXIII function and can be functionally categorized on the basis of their expression phenotype. PMID: 27363989
  11. These findings provide insight into assembly of the fibrinogen/FXIII-A2B2 complex in both physiologic and therapeutic situations. PMID: 27561317
  12. FXIIIa exhibits a preference for Q237 in crosslinking reactions within fibrinogen alphaC (233-425) followed by Q328 and Q366. PMID: 26951791
  13. factor XIII Val34Leu polymorphism is associated with coronary artery diseases risk, especially myocardial infarction. Age and sex did not affect the relationship between factor XIII Val34Leu polymorphism and diseases risk (Meta-Analysis) PMID: 27665853
  14. Mounting evidence now suggests that platelet FXIII-A modulates hemostasis by several different mechanisms. This condensed review discusses recent advances in the understanding of the novel intracellular and extracellular functions of platelet FXIII-A. [review] PMID: 27207415
  15. It is a unique case showing the combination of a highly aggressive angiosarcoma and presence of inherited FXIII deficiency. It is also a rare example demonstrating the benefit of FXIII genotyping besides the expected acquired FXIII deficiency possibly due to neoplasm induced increased consumption by elevated crosslinking of fibrin fibers. PMID: 26540128
  16. The Val34Leu polymorphism of FXIII was not found in Korean people, and compared with Caucasians, a noticeably low incidence of deep vein thrombosis was shown. PMID: 26802299
  17. Genetic basis of severe factor XIII deficiency in a large cohort of Indian patients has been uncovered. PMID: 26852661
  18. Our meta-analysis suggests that there is no evidence for strong association between FXIII Val34Leu polymorphisms and intracerebral hemorrhage--{review} PMID: 26121426
  19. Deletion of 11 or more N-terminal amino acids disrupts intersubunit interactions, which may prevent FXIII-A2 homodimer formation. AP-FXIII plays an important role in the stability of the FXIII-A2 dimer. PMID: 26083359
  20. Mutations in the activation peptide of full-length recombinant FXIII regulate activation rates by thrombin, and V34L influences in vivo thrombus formation by increased cross-linking of the clot. PMID: 26743168
  21. Different FXIII-A dynamics and levels could be utilised as early prognostic indicators during acute MI, revealing the individual potential to heal and suggesting tailored treatments to avoid heart failure or its extreme consequence. PMID: 25947356
  22. FXIII Val34Leu polymorphism has a protective effect against recurrent spontaneous abortion. PMID: 25862345
  23. Report immunochromatographic test for detection of anti-factor XIII A subunit antibodies that can diagnose 90 % of cases with autoimmune haemorrhaphilia XIII. PMID: 25740658
  24. These results indicate that FXIIIa activity can be modulated by fibrinolytic enzymes, and suggest that changes in fibrinolytic activity may influence cross-linking of blood proteins. PMID: 26359437
  25. Suggest that FXIIIa positive dermal dendrocytes may be the main antigen presenting cells in indeterminate leprosy. PMID: 25365500
  26. there is an evidence for association between factor XIII Val34Leu polymorphism and CSX PMID: 23677728
  27. These findings expose a newly recognized, essential role for fibrin crosslinking during whole blood clot formation and consolidation and establish FXIIIa activity as a key determinant of thrombus composition and size. PMID: 26324704
  28. Results show that FXIII-B Arg95 variant is associated with an increased risk of abdominal aortic aneurysms (AAA) suggesting a possible role for FXIII in AAA pathogenesis. PMID: 25384012
  29. The results demonstrate that FXIII-A Val34Leu polymorphism does not influence the occurrence of atherothrombotic ischemic stroke, but has an effect on the severity of its outcome. PMID: 24686102
  30. His343Gln was a novel missense mutation occurring in the core domain of the FXIII A subunit. This is the first report of genetically confirmed FXIII deficiency in Korea, with novel and recurrent F13A1 mutations. PMID: 25004025
  31. study suggested that FXIIIA Val34Leu polymorphism was a protective factor for myocardial infarction in caucasians PMID: 24042156
  32. Genotype 163TT of FXIII-A gene as a new independent risk factor for the development of Venous Thromboembolism in young women living in the North-West region of Russia. PMID: 26035561
  33. This study presents the covalent structure of single-stranded fibrin oligomers cross-linked by FXIIIa. PMID: 25896761
  34. a cohort of 27 individuals was analysed and four novel mutations leading to congenital FXIII deficiency were reported PMID: 24329762
  35. FXIII-A has a functional role through exposure on the activated platelet membrane where it exerts antifibrinolytic function by cross-linking alpha2AP to fibrin PMID: 25331118
  36. 8 new heterozygous missense mutations (Pro166Leu, Arg171Gln, His342Tyr, Gln415Arg, Leu529Pro, Gln601Lys, Arg703Gln and Arg715Gly) may affect catalysis, barrel domain integrity or activation peptide cleavage, depending on domain. PMID: 24889649
  37. results demonstrate the utility of eQTL mapping in the identification of novel asthma genes and provide evidence for the importance of FADS2, NAGA, and F13A1 in the pathogenesis of asthma. PMID: 24934276
  38. FXIII Val34Leu and PAI-1 4G/5G polymorphisms are prevalent in Egyptian women, with unexplained primary first trimester Recurrent miscarriage and combined polymorphisms statistically increasing the risk. PMID: 24702949
  39. FXIII-A serves as a preadipocyte-bound proliferation/differentiation switch that mediates effects of hepatocyte-produced circulating pFN. PMID: 24934257
  40. plasma FN assembly into bone matrix in vitro requires FXIIIA transglutaminase activity making pFN assembly an active, osteoblast-mediated process. PMID: 24246248
  41. this study presents for the first time an in depth and time-resolved analysis of the FXIIIa substrate proteome in plasma. PMID: 24443567
  42. Patients with advanced-stage NSCLC exhibited higher coagulation FXIII activity than healthy controls and early-stage NSCLC patients. PMID: 24142643
  43. These data suggest that plasma transglutaminase factor XIII may play a key role in fetal development of vertebrates via cross-link of Fas antigen. PMID: 24216108
  44. Data suggest that Factor XIII (composed of subunits F13A and F13B) increases rigidity/strength of fibrin clot, protects fibrin clot against shear stress in circulation, and protects fibrin from prompt elimination by fibrinolytic system. [REVIEW] PMID: 24476525
  45. Studies indicate that mutations in the factor XIII-A (FXIII-A) gene causing congenital factor XIII deficiency. PMID: 23929307
  46. Overproduction of FXIII-A by M2 macrophages might contribute to the excessive fibrin deposition in the submucosa of nasal polyps (NP), which might contribute to the tissue remodeling and pathogenesis of chronic rhinosinusitis with NP. PMID: 23541322
  47. The Fctor XIIIa R260C mutant has significantly altered conformations, resulting in the rapid degradation by the proteasome inside the synthesizing cells PMID: 23279035
  48. No association has been found between FXIII-A Val34Leu genotype and the risk for peripheral arterial disease in a Hungarian cohort of patients. PMID: 23518792
  49. The prevalence of a F13A1 gene intron 1 variant (IVS1+12C>A) was found with greater frequency in patients with a mild FXIII deficiency with no detectable F13A1 or F13 B mutations than in those with heterozygous F13A1 mutations or in normal controls. PMID: 23508224
  50. The results suggest an increase in FXIII activity as the number of repetitions of the short tandem repeat polymorphism in the F13A01 gene increased up to allele 5. PMID: 22909824
  51. genetic association studies in a population in Hungary: Data suggest that Leu34Leu homozygous variant of FXIII subunit A Val34Leu polymorphism significantly increases risk of fatal primary intracerebral hemorrhagic stroke in men. PMID: 22909010
  52. The Val34Leu polymorphism in the f13a1 gene is associated with increased plasma factor XIII activation and seems to be linked with impaired cutaneous microcirculation in patients with diabetic foot ulcers. PMID: 22578139
  53. The activation peptide cleft exposed by thrombin cleavage of FXIII-A(2) contains a recognition site for the fibrinogen alpha chain. PMID: 23303819
  54. show abundant expression of FXIII-A at the mRNA and protein levels within stenotic aortic valves, which is associated with the severity of aortic stenosis PMID: 23052999
  55. This meta-analysis indicates that there is no evidence for association between coagulation factor XIII A chain Val34Leu polymorphism and ischemic stroke. PMID: 22154241
  56. It is probable that the impaired tertiary structure of the mutant S708R molecule leads to its instability, which is at least in part responsible for the FXIII deficiency of this patient PMID: 22633530
  57. Factor XIII levels are decreased in Crohn's disease patients, but did not correlate with the time course of disease evolution, CRP, serum fibrin levels, platelet count, disease distribution within the bowel, or the presence of a fistulising form. PMID: 22398040
  58. Studies indicate the importance of FXIII-A in thrombosis and wound healing is emphasized in FXIII deficiency, which is characterized by bleeding and abnormal wound healing. PMID: 23075332
  59. Human inter-alpha-inhibitor is a substrate for factor XIIIa and tissue transglutaminase. PMID: 21939789
  60. The Arg77Cys missense mutation and Arg174stop nonsense mutation both severely disturb the normal expression of FXIIIA protein. PMID: 22169303
  61. a nocvel expression site of FXIII-A in acute promyelocytic leukemia M3 can be considered as a leukemia associated immunophenotype and may have pathophysiological significance. PMID: 22434605
  62. F13A1 single nucleotide polymorphism is associated with polycystic ovary syndrome. PMID: 22565190
  63. Letter: suggest that recurrent pregnancy loss in the general population is not associated with reduced FXIII plasma levels. PMID: 22329719
  64. 5 novel missense mutations; 2, Arg77His and Trp187Arg, were the most common in Iranian FXIII-deficient patients (Review) PMID: 22156982
  65. data suggest that decrease of factor XIII chain A might be associated with development of preeclampsia PMID: 22234358
  66. Fibrinogen plasma levels, the fibrinogen -455G>A and the FXIII-A Val34Leu polymorphisms were not associated with cancer-associated venous thromboembolism. PMID: 21901234
  67. factor XIII Val34Leu genetic variation in the A subunit is not associated with recurrent spontaneous abortion PMID: 21548851
  68. Identification of respective lysine donor and glutamine acceptor sites involved in factor XIIIa-catalyzed fibrin alpha chain cross-linking. PMID: 22030394
  69. genetic polymorphism is associated with early-onset myocardial infarction in the Italian population PMID: 21901231
  70. A review analyzes and present an exhaustive amount of F13B mutational data from the past three decades. PMID: 21640452
  71. Eight mutations were detected in these 7 patients of whom five were missense (Gln229Arg, Arg681Gln, Arg260His, Ser19Pro, and Ser263Pro), two were nonsense (Arg174X, Gln85X) and a single heterozygous deletion c.173delG resulting in a frame shift mutation PMID: 21658166
  72. FXIIIa and TG2 act similarly on glutamines at either end of (1-5)F1 and transamidation specificity of both enzymes is achieved through interactions with the intact 29K fragment. PMID: 21757696
  73. lower expression in embolised compared to non-embolised thrombi in atrial fibrillation patients PMID: 21655673
  74. the first 951 bp of F13A1 intron 1 is involved in regulating the expression of the F13A1 gene PMID: 21512576
  75. The metals 1 mM Mg2+, 1 mM Ba2+, and 1 mM Cu2+ each led to conformational changes, many in the same FXIII regions as Ca2+. PMID: 21640701
  76. Eight novel F13A mutations are analyzed by direct sequencing among 13 patients of Caucasian origin with clinically diagnosed FXIII deficiency; while the cause of the nonsense mutations is obvious, that of the missense mutations remains less clear. PMID: 20179087
  77. the FXIII-A Leu34 allele may contribute to a protective effect against the development of MI. There is a low prevalence of the Leu34 allele in Han Chinese compared to Caucasians. PMID: 20211923
  78. Polymorphisms of coagulation factor XIII (FXIII) genes and their association with recurrent spontaneous abortion (RSA) in Iranian patients and normal healthy controls. PMID: 20822334
  79. In addition to fibrinogen gamma' chain binding, the fibrin alphaC region also provides a platform for the binding of FXIII-A(2)B(2) and FXIII-A subunit. PMID: 21224475
  80. genetic polymorphism is associated with recurrent pregnancy loss in Iranian women PMID: 20384622
  81. involved in the regulation of gene expression in alternatively activated macrophages PMID: 20664907
  82. Megakaryocyte adhesion to type I collagen promotes inhibits pro-platelet formation through the release of cFN to the plasma membrane. This regulatory mechanism is dependent on the engagement of FN receptors and on transglutaminase FXIII-A activity. PMID: 21131589
  83. Letter/Case Report: report a case of acquired FXIII deficiency with FXIII-specific inhibitor treated with FXIII concentrates, which subsequently resulted in spontaneous disappearance of the inhibitor. PMID: 20941461
  84. Coagulation factor XIII A Val34Leu polymorphism fails to influence the risk of atherothrombotic ischemic stroke. PMID: 20609463
  85. The light factor XIII deficiency in one patient is coincidental and not the sole cause of bleeding. PMID: 20680227
  86. Like in healthy subjects, in patients scheduled for CABG, the FXIII Leu34 allele is associated with decreased fibrin clot permeability and efficiency of lysis. PMID: 19784898
  87. A molecule indistinguishable from RP S19 was present in plasma, and that the RP S19-like molecule was converted to the active form by a transglutaminase-catalyzed reaction. PMID: 20093496
  88. FXIII-A associated with podosomes & other structures adjacent to the plasma membrane, containing TGN46 & GMP-130 (GM130) but not protein disulphide isomerase. FXIII-A was present in GM130-positive intracellular vesicles that could mediate its transport. PMID: 20086247
  89. In probands and in eight family members with congenital factor XIII deficiency in Tunisia, FXIIIA subunit activity is undetectable, while the FXIIIB subunit is within normal range. PMID: 19937244
  90. findings indicate that the common FXIII Val34Leu polymorphism is associated with differences in monocyte and neutrophil cell counts in response to systemic LPS infusion PMID: 19787322
  91. investigated FXIII levels in three consecutive patients presenting with acute myocardial rupture following myocardial infarction. FXIII levels in these patients were only 5768% of normal PMID: 17183677
  92. FXIIIa substrate, A2AP, sequences suggests that the residue located two positions beyond the reactive glutamine is not conserved. this position makes important contributions to effective FXIIIa-A2AP interactions PMID: 19691486
  93. Introduction of an optimal thrombin cleavage site, V34L+V35T, increased the activation rate 7.6-fold and facilitated the formation of a fibrin network more resistant to fibrinolysis than obtained with wt FXIII-A. PMID: 19804366
  94. Polymorphisms of coagulation factor XIII subunit A and risk of hemorrhagic stroke in young white women. PMID: 11692020
  95. The results suggest that the Leu 34 allele of the A-chain factor XIII gene has a minor role in the development of non-traumatic primary intracerebral haemorrhage. PMID: 11920201
  96. A common polymorphism in the gene for factor XIII A subunit (F13A1 V34L) has been associated with a decreased risk for coronary artery disease, cerebrovascular disease, and deep venous thrombosis. PMID: 11941274
  97. Numbers of epidermal and dermal CD1a(+) cells and factor XIIIa(+) cells were significantly greater than in normal control skin, while in psoriasis only factor XIIIa(+) cells were significantly increased in number. PMID: 12373334
  98. the associations of factor XIIIA and XIIIB polymorphisms and their interactions with estrogen therapy on risk of nonfatal myocardial infarction PMID: 12456499
  99. activation caused by a specific mutation of neighboring thrombin cleavage site(s) in the activation peptide of FXIII-A like V34L resulted in the real-time amount of the activated factor XIII-A PMID: 12526104
  100. Valproate induces reversible factor XIII deficiency with risk of perioperative bleeding. PMID: 12859294

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Involvement in disease Factor XIII subunit A deficiency (FA13AD)
Subcellular Location Cytoplasm, Secreted
Protein Families Transglutaminase superfamily, Transglutaminase family
Database Links

HGNC: 3531

OMIM: 134570

KEGG: hsa:2162

STRING: 9606.ENSP00000264870

UniGene: Hs.335513

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