Recombinant Human Collagen alpha-1 (XII) chain (COL12A1), partial

Code CSB-YP859521HU
MSDS
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Source Yeast
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Code CSB-EP859521HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP859521HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP859521HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
COL12A1
Uniprot No.
Alternative Names
COL12A1; COL12A1LCollagen alpha-1(XII) chain
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Type XII collagen interacts with type I collagen-containing fibrils, the COL1 domain could be associated with the surface of the fibrils, and the COL2 and NC3 domains may be localized in the perifibrillar matrix.
Gene References into Functions
  1. The study indicates that the spectrum of causative genes in extracellular matrix-related myopathies be extended to include COL12A1. PMID: 24334769
  2. collagen alpha-1 (XII) chain was expressed at dramatically higher (~10-fold) levels in breast PMID: 24262153
  3. Type XII collagen is overexpressed in permanent human and mouse corneal scars and could represent a new target to treat corneal scarring. PMID: 22969073
  4. In addition to binding collagen I, COMP binds to collagens XII and XIV via their C-terminal collagenous domains. PMID: 22573329
  5. COL12A1 is associated with anterior cruciate ligament ruptures among females PMID: 19443461
  6. this study identifies COL12A1 as the likely gene candidate within the recurrent 6q13 breakpoint and provides an alternative approach for detecting 6q13 anomalies in nondividing cells of chondromyxoid fibroma. PMID: 19648885
  7. induction of expression by sheare stress is associated with atherogenesis PMID: 12890494
  8. Results suggest that collagens XII and XIV might serve different functions during human embryonic development although their structures are highly similar. PMID: 15609093

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Involvement in disease
Ullrich congenital muscular dystrophy 2 (UCMD2); Bethlem myopathy 2 (BTHLM2)
Subcellular Location
Secreted, extracellular space, extracellular matrix.
Protein Families
Fibril-associated collagens with interrupted helices (FACIT) family
Tissue Specificity
Found in collagen I-containing tissues: both isoform 1 and isoform 2 appear in amnion, chorion, skeletal muscle, small intestine, and in cell culture of dermal fibroblasts, keratinocytes and endothelial cells. Only isoform 2 is found in lung, placenta, ki
Database Links

HGNC: 2188

OMIM: 120320

KEGG: hsa:1303

STRING: 9606.ENSP00000325146

UniGene: Hs.101302

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