Product Details

Purity

>85% (SDS-PAGE)

Target Names

COL25A1

Uniprot No.

Q9BXS0

Alternative Names

COL25A1Collagen alpha-1(XXV) chain; Alzheimer disease amyloid-associated protein; AMY; CLAC-P) [Cleaved into: Collagen-like Alzheimer amyloid plaque component; CLAC)]

Species

Homo sapiens (Human)

Source

Yeast

Protein Length

Extracellular domain

Tag Info

N-terminal His-tagged
Tag-Free

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Target Background

Function

Inhibits fibrillization of amyloid-beta peptide during the elongation phase. Has also been shown to assemble amyloid fibrils into protease-resistant aggregates. Binds heparin.

Gene References into Functions

We highlight phenotypes of the 4 affected children from the 2 reported families: isolated congenital ptosis (one unilateral, one bilateral) and Duane syndrome (one unilateral, one bilateral) with synergistic divergence. PMID: 26486031
We identified COL25A1 mutations as a cause of autosomal-recessive congenital cranial dysinnervation disorder. PMID: 25500261
COL25A1 methylation correlates with severity of cervical intraepithelial neoplasia PMID: 23018867
The COL25A1 single nucleotide polymorphism rs13134663 was significantly associated with antisocial personality disorder, especially in subjects with comorbid substance dependence. PMID: 22297151
The next best locus for gene x gender interactions for age at onset was in COL25A1 gene at 4q25 PMID: 21688384
provides genetic evidence of association between COL25A1 and risk for Alzheimer's disease PMID: 18501477
COL25A1 leads to Alzheimer's disease-like pathology in vivo PMID: 19548013
CLAC displays features characteristic of a collagen protein, ie. it forms a partly protease-resistant triple-helical structure, exhibits an intermediate affinity for heparin, and is glycosylated; binding domain between CLAC and amyloid beta-peptide PMID: 15522881
CLACbinds to amyloid beta peptides through the positively charged amino acid cluster within the collagenous domain 1 and inhibits formation of amyloid fibrils PMID: 15615705
Results show that collagenous Alzheimer amyloid plaque component (CLAC) assembles amyloid-beta fibrils into fibril bundles that have an increased resistance to proteases. PMID: 15853808
suggest that CLAC becomes involved at an intermediate stage in the pathogenesis of Alzheimer's disease by binding to Abeta fibrils, including fibrils formed from peptides with truncated N- or C-termini, and thereby slows their growth PMID: 16300410