Recombinant Human Collagen and calcium-binding EGF domain-containing protein 1 (CCBE1), partial

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Code CSB-MP747637HU1
Abbreviation Recombinant Human CCBE1 protein, partial
MSDS
Size $318
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity
Greater than 95% as determined by SDS-PAGE.
Activity
Not Test
Target Names
CCBE1
Uniprot No.
Research Area
Others
Alternative Names
Full of fluid protein homolog
Species
Homo sapiens (Human)
Source
Mammalian cell
Expression Region
159-250aa
Target Protein Sequence
CECREGYIREDDGKTCTRGDKYPNDTGHEKSENMVKAGTCCATCKEFYQMKQTVLQLKQKIALLPNNAADLGKYITGDKVLASNTYLPGPPG
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
Mol. Weight
39.0 kDa
Protein Length
Partial
Tag Info
C-terminal hFc1-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Required for lymphangioblast budding and angiogenic sprouting from venous endothelium during embryogenesis.
Gene References into Functions
  1. the present results demonstrated that CCBE1 expression was downregulated in lung cancer, particularly in the presence of LNM. PMID: 29207117
  2. these data indicated that CCBE1 may be served as a new predictor of prognosis in post-operative gastrointestinal stromal tumor patients and may play an important role in stimulating tumor progression PMID: 27506146
  3. We identify CCBE1 as a direct target of miR-330-3p, and show that knockdown of CCBE1 results in a greater invasive capacity. Accordingly, in breast cancer patients CCBE1 is frequently downregulated, and its loss is associated with reduced distant relapse-free and overall survival. PMID: 28419078
  4. characterization of Hennekam Syndrome phenotypes in two Turkish siblings with protein mutation PMID: 26686525
  5. Collagen domains of CCBE1 are crucial for the activation of VEGFC in vitro and in vivo. The EGF domains of CCBE1 are dispensable for regulation of VEGFC processing in vitro, however, they are necessary for full lymphangiogenic activity of CCBE1 in vivo. PMID: 25814692
  6. CCBE1 enhances lymphangiogenesis via A disintegrin and metalloprotease with thrombospondin motifs-3-mediated vascular endothelial growth factor-C activation. PMID: 24552833
  7. Both siblings harbored a homozygous mutation in CCBE1. PMID: 24086631
  8. The study has shown that CCBE1 mutations are not a major contributor to non-immune hydrops fetalis. PMID: 22239599
  9. Human CCBE1 strongly enhances vascular endothelial growth factor-C-mediated lymphangiogenesis in a corneal micropocket assay PMID: 21778431
  10. Loss of CCBE1 expression may promote ovarian carcinogenesis by enhancing migration & cell survival. CCBE1 is a new candidate tumour suppressor in ovarian cancer. PMID: 19935792
  11. Homozygous cysteine to serine change and SNPS in CCBE1 were identified patients. PMID: 19911200
  12. Mutations in CCBE1 cause generalized lymph vessel dysplasia in humans PMID: 19935664

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Involvement in disease
Hennekam lymphangiectasia-lymphedema syndrome 1 (HKLLS1)
Subcellular Location
Secreted.
Protein Families
CCBE1 family
Tissue Specificity
Not expressed in blood or lymphatic endothelial cells.
Database Links

HGNC: 29426

OMIM: 235510

KEGG: hsa:147372

STRING: 9606.ENSP00000404464

UniGene: Hs.34333

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