Recombinant Human Coronin-7 (CORO7), partial

Code CSB-YP005819HU
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP005819HU
MSDS
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Source E.coli
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Code CSB-EP005819HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP005819HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP005819HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
CORO7
Uniprot No.
Alternative Names
0610011B16Rik; 70 kDa WD repeat tumor rejection antigen homolog; CORO 7; Coro7; CORO7_HUMAN; Coronin-7; Coronin7; Crn7; FLJ22021; FLJ44188; POD1
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
F-actin regulator involved in anterograde Golgi to endosome transport: upon ubiquitination via 'Lys-33'-linked ubiquitin chains by the BCR(KLHL20) E3 ubiquitin ligase complex, interacts with EPS15 and localizes to the trans-Golgi network, where it promotes actin polymerization, thereby facilitating post-Golgi trafficking. May play a role in the maintenance of the Golgi apparatus morphology.
Gene References into Functions
  1. Obese children had significantly lower methylation levels at a CpG site located near coronin 7 (CORO7), which encodes a tryptophan-aspartic acid dipeptide (WD)-repeat containing protein most likely involved in Golgi complex morphology and function PMID: 25887538
  2. Results show that enforced targeting of Crn7 to TGN 9trans-Golgi network) bypasses the requirement of K33-ubiquitination for TGN-pool F-actin assembly and post-Golgi trafficking. PMID: 24768539
  3. Data show that CRN7 interacted with Cullin1 and Roc1 to form a novel SCF-like E3 complex. PMID: 21130766
  4. role in the organization of intracellular membrane compartments and vesicular trafficking rather than in remodeling the cytoskeleton PMID: 15327992
  5. Crn7 interacts with AP-1 and is required for the maintenance of Golgi morphology and protein export from the Golgi PMID: 16905771
  6. Sequential events of protein interaction and posttranslational modification result in the membrane targeting of Crn7. PMID: 18581049

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Subcellular Location
Golgi apparatus membrane. Golgi apparatus, trans-Golgi network. Cytoplasmic vesicle. Cytoplasm, cytosol. Note=Predominantly cytosolic. Detected on vesicle-like cytoplasmic structures and on the cis-Golgi. Not associated with actin filaments.
Protein Families
WD repeat coronin family
Tissue Specificity
Widely expressed. Expressed in the spleen, peripheral leukocytes, testes, brain, thymus and small intestine.
Database Links

HGNC: 26161

OMIM: 611668

KEGG: hsa:79585

STRING: 9606.ENSP00000460885

UniGene: Hs.437957

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