Product Details

Purity

>85% (SDS-PAGE)

Target Names

CPEB3

Uniprot No.

Q8NE35

Alternative Names

CPE-binding protein 3; CPE-BP3; CPEB 3; CPEB3; CPEB3_HUMAN; Cytoplasmic polyadenylation element binding protein 3; Cytoplasmic polyadenylation element-binding protein 3; hCPEB-3; KIAA0940

Species

Homo sapiens (Human)

Expression Region

1-698

Target Protein Sequence

MQDDLLMDKS KTQPQPQQQQ RQQQQPQPES SVSEAPSTPL SSETPKPEEN SAVPALSPAA APPAPNGPDK MQMESPLLPG LSFHQPPQQP PPPQEPAAPG ASLSPSFGST WSTGTTNAVE DSFFQGITPV NGTMLFQNFP HHVNPVFGGT FSPQIGLAQT QHHQQPPPPA PAPQPAQPAQ PPQAQPPQQR RSPASPSQAP YAQRSAAAAY GHQPIMTSKP SSSSAVAAAA AAAAASSASS SWNTHQSVNA AWSAPSNPWG GLQAGRDPRR AVGVGVGVGV GVPSPLNPIS PLKKPFSSNV IAPPKFPRAA PLTSKSWMED NAFRTDNGNN LLPFQDRSRP YDTFNLHSLE NSLMDMIRTD HEPLKGKHYP PSGPPMSFAD IMWRNHFAGR MGINFHHPGT DNIMALNNAF LDDSHGDQAL SSGLSSPTRC QNGERVERYS RKVFVGGLPP DIDEDEITAS FRRFGPLVVD WPHKAESKSY FPPKGYAFLL FQEESSVQAL IDACLEEDGK LYLCVSSPTI KDKPVQIRPW NLSDSDFVMD GSQPLDPRKT IFVGGVPRPL RAVELAMIMD RLYGGVCYAG IDTDPELKYP KGAGRVAFSN QQSYIAAISA RFVQLQHNDI DKRVEVKPYV LDDQMCDECQ GTRCGGKFAP FFCANVTCLQ YYCEYCWASI HSRAGREFHK PLVKEGGDRP RHVPFRWS

Protein Length

full length protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Sequence-specific RNA-binding protein which acts as a translational repressor in the basal unstimulated state but, following neuronal stimulation, acts as a translational activator. In contrast to CPEB1, does not bind to the cytoplasmic polyadenylation element (CPE), a uridine-rich sequence element within the mRNA 3'-UTR, but binds to a U-rich loop within a stem-loop structure. Required for the consolidation and maintenance of hippocampal-based long term memory. In the basal state, binds to the mRNA 3'-UTR of the glutamate receptors GRIA2/GLUR2 mRNA and negatively regulates their translation. Also represses the translation of DLG4, GRIN1, GRIN2A and GRIN2B. When activated, acts as a translational activator of GRIA1 and GRIA2. In the basal state, suppresses SUMO2 translation but activates it following neuronal stimulation. Binds to the 3'-UTR of TRPV1 mRNA and represses TRPV1 translation which is required to maintain normal thermoception. Binds actin mRNA, leading to actin translational repression in the basal state and to translational activation following neuronal stimulation. Negatively regulates target mRNA levels by binding to TOB1 which recruits CNOT7/CAF1 to a ternary complex and this leads to target mRNA deadenylation and decay. In addition to its role in translation, binds to and inhibits the transcriptional activation activity of STAT5B without affecting its dimerization or DNA-binding activity. This, in turn, represses transcription of the STAT5B target gene EGFR which has been shown to play a role in enhancing learning and memory performance. In contrast to CPEB1, CPEB2 and CPEB4, not required for cell cycle progression.

Gene References into Functions

Overexpression of miR-452-3p promoted cell proliferation and mobility and suppressed apoptosis. MiR-452-3p enhanced EGFR and phosphorylated AKT (pAKT) expression but inhibited p21 expression level. MiR-452-3p promoted hepatocellular carcinoma (HCC)cell proliferation and mobility by directly targeting the CPEB3/EGFR axis PMID: 29332449
Data show that the expression of cytoplasmic polyadenylation element binding protein 3 (CPEB3) positively correlated with tumor progression and malignancy but negatively correlated with protein phosphorylation in the alternatively spliced region. PMID: 27256982
Data show that EGF receptor (EGFR) is involved in the microRNA miR-107 pathogenesis of hepatocellular carcinoma (HCC) through cytoplasmic polyadenylation element binding protein 3 (CPEB3). PMID: 26497556
Data suggest that the RNA recognition motif (RRM) domain of CPEB3 (as a soluble peptide fragment) exhibits protein conformation distinct from those of the canonical RRM domain. PMID: 25066254
cleavage of CPEB3 by NMDA-activated calpain 2 accounts for the activity-related translation of CPEB3-targeted RNAs PMID: 22711986
Secondary structure determination and native gel analyses reveal that the cleaved population of the CPEB3 ribozyme has a single, secondary structure that closely resembles the HDV ribozyme PMID: 20524672
isolation of a ribozyme conserved mammalian sequence that resides in an intron of the CPEB3 gene PMID: 16990549

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Subcellular Location

Cytoplasm. Nucleus. Cell junction, synapse. Cell projection, dendrite. Cell junction, synapse, postsynaptic density.

Protein Families

RRM CPEB family

Database Links

HGNC: 21746

OMIM: 610606

KEGG: hsa:22849

STRING: 9606.ENSP00000265997

UniGene: Hs.131683

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Recombinant Human Cytoplasmic polyadenylation element-binding protein 3 (CPEB3)

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