Recombinant Human Cytosolic 5'-nucleotidase 3 (NT5C3)

Code CSB-YP867122HU
MSDS
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Source Yeast
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Code CSB-EP867122HU
MSDS
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Source E.coli
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Code CSB-EP867122HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP867122HU
MSDS
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Source Baculovirus
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Code CSB-MP867122HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
NT5C3A
Uniprot No.
Alternative Names
5NT3A_HUMAN; cN III ; cN-III; cNIII ; Cytosolic 5' nucleotidase 3; Cytosolic 5' nucleotidase III; Cytosolic 5''-nucleotidase 3; Cytosolic 5''-nucleotidase 3A; Cytosolic 5''-nucleotidase III; NT5C3; Nt5c3a; p36; P5''N-1; P5'N 1 ; P5'N1 ; P5N 1 ; P5N-1; P5N1 ; PN I ; PN-I; PNI ; PSN1 ; Pyrimidine 5' nucleotidase 1; Pyrimidine 5''-nucleotidase 1; UMPH ; UMPH1 ; Uridine 5' monophosphate hydrolase 1; Uridine 5''-monophosphate hydrolase 1
Species
Homo sapiens (Human)
Expression Region
1-336
Target Protein Sequence
MRAPSMDRAA VARVGAVASA SVCALVAGVV LAQYIFTLKR KTGRKTKIIE MMPEFQKSSV RIKNPTRVEE IICGLIKGGA AKLQIITDFD MTLSRFSYKG KRCPTCHNII DNCKLVTDEC RKKLLQLKEK YYAIEVDPVL TVEEKYPYMV EWYTKSHGLL VQQALPKAKL KEIVAESDVM LKEGYENFFD KLQQHSIPVF IFSAGIGDVL EEVIRQAGVY HPNVKVVSNF MDFDETGVLK GFKGELIHVF NKHDGALRNT EYFNQLKDNS NIILLGDSQG DLRMADGVAN VEHILKIGYL NDRVDELLEK YMDSYDIVLV QDESLEVANS ILQKIL
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Nucleotidase which shows specific activity towards cytidine monophosphate (CMP) and 7-methylguanosine monophosphate (m(7)GMP). CMP seems to be the preferred substrate.
Gene References into Functions
  1. Results from a study on gene expression variability markers in early-stage human embryos shows that NT5C3A is a putative expression variability marker for the 3-day, 8-cell embryo stage. PMID: 26288249
  2. reduced expression of transketolase in pyrimidine 5'-nucleotidase deficient patients PMID: 27381654
  3. Pyrimidine-5'-nucleotidase Campinas is a new mutation (p.R56G) in the NT5C3 gene associated with pyrimidine-5'-nucleotidase type I deficiency. PMID: 25153905
  4. Results show that NT5C3 expression levels were significantly increased in patients with risk allele homozygote. PMID: 25000516
  5. New missense homozygous mutation (Q270Ter) in the pyrimidine 5' nucleotidase type I-related gene in two Indian families with hereditary non-spherocytic hemolytic anemia. PMID: 23139015
  6. All investigated mutant proteins display impaired catalytic properties and/or reduced thermostability, providing a rationale for the pathological effects of the mutations. PMID: 15604219
  7. These data highlight hUMP1/POMP role in proteasome assembly and further strengthen the prospect of genetic manipulation of the proteasomal system. PMID: 17349762
  8. 3 newly described missense mutations (c.187T>C, c.469G>C & c.740T>C) identified in patients with hemolytic anemia were characterized at protein level; the enzymes were altered in both thermal stability and catalytic efficiency PMID: 18499901
  9. genetic variation in NT5C3 might affect protein function and potentially influence drug response PMID: 19623099

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Involvement in disease
P5N deficiency (P5ND)
Subcellular Location
Cytoplasm.; [Isoform 2]: Endoplasmic reticulum.
Protein Families
Pyrimidine 5'-nucleotidase family
Tissue Specificity
Isoforms 1, 3 and 4 are expressed in reticulocytes. Isoform 4 is hardly detectable in bone marrow and fetal liver.
Database Links

HGNC: 17820

OMIM: 266120

KEGG: hsa:51251

STRING: 9606.ENSP00000242210

UniGene: Hs.487933

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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