Recombinant Human D-amino-acid oxidase (DAO) gets expressed in E. coli and matches the complete amino acid sequence (1-347aa) found in the human protein. This product comes with an N-terminal 6xHis-SUMO tag, which makes purification and detection more straightforward. The protein reaches greater than 90% purity, as confirmed by SDS-PAGE analysis. This appears to provide high-quality material suitable for research work. Worth noting - this product is meant strictly for research purposes.
D-amino-acid oxidase (DAO) is an enzyme that handles the oxidative deamination of D-amino acids, turning them into their corresponding imino acids. The enzyme likely plays an important role in amino acid breakdown and participates in several metabolic pathways. DAO has caught researchers' attention, particularly those studying its function in brain processes and its possible connections to neurological disorders.
Potential Applications
Note: The applications listed below are based on what we know about this protein's biological functions, published research, and experience from experts in the field. However, we haven't fully tested all of these applications ourselves yet. We'd recommend running some preliminary tests first to make sure they work for your specific research goals.
1. Enzyme Kinetics and Substrate Specificity Studies
This recombinant human DAO works well for characterizing the enzyme's kinetic parameters and substrate preferences under controlled lab conditions. Scientists can track enzyme activity using different D-amino acid substrates to figure out Km, Vmax, and kcat values. The high purity (>90%) may help ensure accurate kinetic measurements without interference from other proteins. That N-terminal His-SUMO tag makes purification easier and allows for immobilization during repeated testing.
2. Inhibitor Screening and Drug Discovery Research
The purified DAO protein appears to be an ideal target for testing potential inhibitors in pharmaceutical research. Small molecule libraries can be screened against the enzyme to find compounds that change DAO activity. The His-tag allows for simple protein capture in high-throughput screening setups like 96-well or 384-well plates. This supports early-stage research into compounds that might influence D-amino acid metabolism.
3. Antibody Development and Validation
The full-length recombinant human DAO protein works as an antigen for creating specific antibodies against human DAO. Its high purity seems suitable for immunization protocols in antibody production. The protein can also function as a positive control and standard when validating newly developed anti-DAO antibodies in Western blotting, ELISA, and immunoprecipitation experiments.
4. Protein-Protein Interaction Studies
The His-SUMO tagged DAO can be used in pull-down assays to find potential binding partners or regulatory proteins that might interact with human DAO. The dual tagging system offers flexibility for different capture approaches - either His-tag affinity chromatography or SUMO-specific interactions. Cell lysates or purified protein libraries can be tested to map out the DAO interactome and better understand how it gets regulated within cells.
5. Structural and Biophysical Characterization
This recombinant protein preparation makes detailed structural studies possible, including X-ray crystallography, NMR spectroscopy, and cryo-electron microscopy experiments. The high purity and full-length characteristics may make it well-suited for biophysical analyses like dynamic light scattering, differential scanning calorimetry, and circular dichroism spectroscopy. These studies could reveal insights into protein folding, stability, and how the protein changes shape under various experimental conditions.
