Recombinant Human DET1 homolog (DET1)

Code CSB-YP762572HU
MSDS
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Source Yeast
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Code CSB-EP762572HU
MSDS
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Source E.coli
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Code CSB-EP762572HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP762572HU
MSDS
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Source Baculovirus
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Code CSB-MP762572HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
DET1
Uniprot No.
Alternative Names
DET1; DET1 homolog; De-etiolated-1 homolog
Species
Homo sapiens (Human)
Expression Region
1-550
Target Protein Sequence
MDHHVSTIKP RRIQNQNVIH RLERRRISSG KAGTHWHQVR VFHQNVFPNF TVVNVEKPPC FLRKFSPDGR YFIAFSSDQT SLEIYEYQGC QAAEDLLQGY EGEILSNGND QRSVNIRGRL FERFFVLLHI TNVAANGEHL NRECSLFTDD CRCVIVGSAA YLPDEPHPPF FEVYRNSESV TPNPRSPLED YSLHIIDLHT GRLCDTRTFK CDKVVLSHNQ GLYLYKNILA ILSVQQQTIH VFQVTPEGTF IDVRTIGRFC YEDDLLTVSA VFPEVQRDSQ TGMANPFRDP FINSLKHRLL VYLWRRAEQD GSAMAKRRFF QYFDQLRQLR MWKMQLLDEN HLFIKYTSED VVTLRVTDPS QASFFVVYNM VTTEVIAVFE NTSDELLELF ENFCDLFRNA TLHSEVQFPC SASSNNFARQ IQRRFKDTII NAKYGGHTEA VRRLLGQLPI SAQSYSGSPY LDLSLFSYDD KWVSVMERPK TCGDHPIRFY ARDSGLLKFE IQAGLLGRPI NHTVRRLVAF TFHPFEPFAI SVQRTNAEYV VNFHMRHCCT
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Component of the E3 ubiquitin ligase DCX DET1-COP1 complex, which is required for ubiquitination and subsequent degradation of target proteins. The complex is involved in JUN ubiquitination and degradation.
Gene References into Functions
  1. promotes ubiquitination and degradation of c-Jun by assembling a multisubunit ubiquitin ligase containing DNA Damage Binding Protein-1 (DDB1), cullin 4A (CUL4A), Regulator of Cullins-1 (ROC1), and constitutively photomorphogenic-1 PMID: 14739464
  2. These findings demonstrate that the conserved DET1 complex modulates Cul4A functions by a novel mechanism. PMID: 17452440
Subcellular Location
Nucleus.
Protein Families
DET1 family
Tissue Specificity
Highly expressed in the ovary, some lymphoid organs and resting leukocytes.
Database Links

HGNC: 25477

OMIM: 608727

KEGG: hsa:55070

STRING: 9606.ENSP00000408994

UniGene: Hs.655064

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