Recombinant Human Dexamethasone-induced Ras-related protein 1 (RASD1)

Code CSB-YP896868HU
MSDS
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Source Yeast
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Code CSB-EP896868HU
MSDS
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Source E.coli
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Code CSB-EP896868HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP896868HU
MSDS
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Source Baculovirus
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Code CSB-MP896868HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
RASD1
Uniprot No.
Alternative Names
Activator of G protein signaling 1; Activator of G protein signaling; Activator of G-protein signaling 1; AGS 1; AGS1; Dexamethasone induced ras related protein 1; Dexamethasone-induced ras protein 1; Dexamethasone-induced Ras-related protein 1; Dexras 1; MGC:26290; RAS dexamethasone induced 1; Ras protein; Dexamethasone-induced; 1; Ras related protein; RASD 1; Rasd1; RASD1_HUMAN
Species
Homo sapiens (Human)
Expression Region
1-278
Target Protein Sequence
MKLAAMIKKM CPSDSELSIP AKNCYRMVIL GSSKVGKTAI VSRFLTGRFE DAYTPTIEDF HRKFYSIRGE VYQLDILDTS GNHPFPAMRR LSILTGDVFI LVFSLDNRDS FEEVQRLRQQ ILDTKSCLKN KTKENVDVPL VICGNKGDRD FYREVDQREI EQLVGDDPQR CAYFEISAKK NSSLDQMFRA LFAMAKLPSE MSPDLHRKVS VQYCDVLHKK ALRNKKLLRA GSGGGGGDPG DAFGIVAPFA RRPSVHSDLM YIREKASAGS QAKDKERC
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Small GTPase. Negatively regulates the transcription regulation activity of the APBB1/FE65-APP complex via its interaction with APBB1/FE65.
Gene References into Functions
  1. These findings suggest that the upregulation of RASD1 in glioma tissues may play an inhibitory role in tumor expansion, possibly through inactivating the AKT/mTOR signaling pathway. PMID: 28600528
  2. Studies indicate potential roles for Rasd1 small G protein and leptin in TRPC4 cation channel activation. PMID: 26083271
  3. Data indicate that protein kinase A (PKA) phosphorylates only serine-253 amino acid on activator of G-protein signaling 1 protein Dexras1 (RASD1). PMID: 26358293
  4. Loss of RASD1 is associated with prostate cancer. PMID: 24761910
  5. Study showed that Rasd1 and NonO interact at the CRE-site of specific target genes. PMID: 21915321
  6. Rasd1 modulates endogenous renin gene expression by interacting with Ear2. PMID: 21247419
  7. A glucocorticoid response element (GRE) was identified in the 3'-flanking region (2.3 kb downstream of poly(A) signal) of the human Dexras1 gene. A point mutation within the 15-bp GRE abolished this glucocorticoid responsiveness PMID: 12818426
  8. plays an active role in preventing aberrant cell growth PMID: 15184869
  9. yeast two hybrid analysis and co-immunoprecipitation studies in mammalian cells demonstrate a direct interaction between AGS1 and the G(beta1) subunit of heterotrimeric G proteins PMID: 16225846
  10. does not alter the membrane translocation of protein kinase C delta. PMID: 16489124
  11. Dexras1 plays a critical role in shaping the photic phase response curve (PRC) and the signaling events through which it regulates clock entrainment in transgenic mice. PMID: 17167088
  12. dex-ras1 mRNA is not induced by glucocorticoid stimulation in HEK293 cells PMID: 17985234
  13. Dexras1 functions as a suppressor of FE65-APP-mediated transcription, and FE65 tyrosine 547 phosphorylation enhances FE65-APP-mediated transcription, at least in part, by modulating the interaction between FE65 and Dexras1 PMID: 18922798
  14. Epigenetic inactivation of genes, including RASD1, plays a key role in the development of dexamethasone resistance in multiple myeloma. PMID: 19549772

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Subcellular Location
Cell membrane; Lipid-anchor; Cytoplasmic side. Cytoplasm, perinuclear region. Nucleus.
Protein Families
Small GTPase superfamily, RasD family
Tissue Specificity
Expressed in a variety of tissues including heart, cardiovascular tissues, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, gastrointestinal and reproductive tissues.
Database Links

HGNC: 15828

OMIM: 605550

KEGG: hsa:51655

STRING: 9606.ENSP00000225688

UniGene: Hs.25829

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