Recombinant Human Dickkopf-like protein 1 (DKKL1)

Code CSB-YP892450HU
MSDS
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Source Yeast
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Code CSB-EP892450HU
MSDS
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Source E.coli
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Code CSB-EP892450HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP892450HU
MSDS
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Source Baculovirus
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Code CSB-MP892450HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
DKKL1
Uniprot No.
Alternative Names
Cancer/testis antigen 34; CT34; dickkopf like 1; Dickkopf-like protein 1; DKKL1; DKKL1_HUMAN; Protein soggy-1; SGY; SGY-1; SGY1; soggy 1
Species
Homo sapiens (Human)
Expression Region
31-242
Target Protein Sequence
APIHDADAQE SSLGLTGLQS LLQGFSRLFL KGNLLRGIDS LFSAPMDFRG LPGNYHKEEN QEHQLGNNTL SSHLQIDKMT DNKTGEVLIS ENVVASIQPA EGSFEGDLKV PRMEEKEALV PIQKATDSFH TELHPRVAFW IIKLPRRRSH QDALEGGHWL SEKRHRLQAI RDGLRKGTHK DVLEEGTESS SHSRLSPRKT HLLYILRPSR QL
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Involved in fertilization by facilitating sperm penetration of the zona pellucida. May promote spermatocyte apoptosis, thereby limiting sperm production. In adults, may reduce testosterone synthesis in Leydig cells. Is not essential either for development or fertility.
Gene References into Functions
  1. DKKL was overexpressed in colorectal cancer tissue compared with healthy colon. DKKL expression positively correlated with colorectal cancer progression. PMID: 27635108
  2. previously unreported alternative splicing patterns in the genes ZNF419 and DKKL1 PMID: 27826023
  3. Findings suggest that Dkk-3 plays a role in the regulation of cell interactions during retinoic acid-induced neuronal differentiation; effect on apoptosis was also observed upon expression of the related protein Dkkl-1 PMID: 24909558
  4. DKKL1/Dkkl1 may play an important role in testicular development and spermatogenesis and may be an important factor in male infertility. PMID: 22817830
Subcellular Location
Secreted. Cytoplasmic vesicle, secretory vesicle, acrosome.
Tissue Specificity
More highly expressed in adult testis than in fetal testis. Exclusively expressed in the testis (at protein level). Intense expression in stages II, III and IV of spermatogenesis, whereas expression is lower in stage I.
Database Links

HGNC: 16528

OMIM: 605418

KEGG: hsa:27120

STRING: 9606.ENSP00000221498

UniGene: Hs.515855

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