Recombinant Human Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial (DLAT), partial

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Code CSB-EP006926HU
Size $224
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP006926HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) DLAT.
  • Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP006926HU could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) DLAT.
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Product Details

Purity
Greater than 90% as determined by SDS-PAGE.
Target Names
DLAT
Uniprot No.
Research Area
Metabolism
Alternative Names
70 kDa mitochondrial autoantigen of primary biliary cirrhosis; Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; Dihydrolipoamide S Acetyltransferase; Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex; dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex mitochondrial; DLAT; DLTA; E2; E2 component of pyruvate dehydrogenase complex; M2 antigen complex 70 kDa subunit; mitochondrial; ODP2_HUMAN; PBC; PDC E2; PDC-E2; PDCE2; Pyruvate dehydrogenase complex component E2; Pyruvate dehydrogenase complex E2 subunit
Species
Homo sapiens (Human)
Source
E.coli
Expression Region
93-640aa
Target Protein Sequence
KVPLPSLSPTMQAGTIARWEKKEGDKINEGDLIAEVETDKATVGFESLEECYMAKILVAEGTRDVPIGAIICITVGKPEDIEAFKNYTLDSSAAPTPQAAPAPTPAATASPPTPSAQAPGSSYPPHMQVLLPALSPTMTMGTVQRWEKKVGEKLSEGDLLAEIETDKATIGFEVQEEGYLAKILVPEGTRDVPLGTPLCIIVEKEADISAFADYRPTEVTDLKPQVPPPTPPPVAAVPPTPQPLAPTPSAPCPATPAGPKGRVFVSPLAKKLAVEKGIDLTQVKGTGPDGRITKKDIDSFVPSKVAPAPAAVVPPTGPGMAPVPTGVFTDIPISNIRRVIAQRLMQSKQTIPHYYLSIDVNMGEVLLVRKELNKILEGRSKISVNDFIIKASALACLKVPEANSSWMDTVIRQNHVVDVSVAVSTPAGLITPIVFNAHIKGVETIANDVVSLATKAREGKLQPHEFQGGTFTISNLGMFGIKNFSAIINPPQACILAIGASEDKLVPADNEKGFDVASMMSVTLSCDHRVVDGAVGAQWLAEFRKYLE
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
62.2kDa
Protein Length
Partial
Tag Info
N-terminal 6xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
Tris-based buffer,50% glycerol
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description

Advance your metabolism research with the Recombinant Human DLAT protein, a critical component of the pyruvate dehydrogenase complex. This mitochondrial enzyme plays a central role in the production of cellular energy, acting as the dihydrolipoyllysine-residue acetyltransferase, responsible for acetyl group transfer during the conversion of pyruvate to acetyl-CoA.

Our Recombinant Human DLAT protein is derived from E.coli and comprises the 93-640aa expression region, corresponding to a partial segment of the native protein. Featuring an N-terminal 6xHis tag, this protein is easily purified and detected, ensuring optimal performance in your experiments. With a purity greater than 90% as determined by SDS-PAGE, this protein delivers consistent results in various applications. Choose between liquid and lyophilized powder forms to best suit your research needs and experimental design.

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Target Background

Function
The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle.
Gene References into Functions
  1. DLAT interacts with C1QBP in mitochondria. PMID: 26753982
  2. ongoing activation of PDCE2-specific B-cells in primary biliary cirrhosis PMID: 25043065
  3. Results show that DLAT and ACAT2 as upstream acetyltransferases of K76 and K294 in 6PGD protein. PMID: 25042803
  4. These findings were used to identify potentially antigenic sequences within PDC-E2 (an important hepatic autoantigen) that contain a DR0801 motif. PMID: 23543758
  5. Solution structure and characterisation of the human pyruvate dehydrogenase complex core assembly PMID: 20361979
  6. Data suggest that the catalytic site of pyruvate dehydrogenase complex E2 rather than the previously reported lipoyl binding peptide may contain immunodominant epitopes recognized by antimitochondrial antibodies in primary biliary cirrhosis. PMID: 20180236
  7. a novel HLA-A*0201-restricted epitope PDC-E2 165 to 174 was defined in patients with primary biliary cirrhosis. PMID: 12395322
  8. study of facilitated interaction between the pyruvate dehydrogenase kinase isoform 2 and the dihydrolipoyl acetyltransferase PMID: 12816949
  9. model of the pyruvate dehydrogenase complex formed by E2 and E2 plus the E3-binding protein and binding of the E1 and E3 components PMID: 14638692
  10. Having found that there is an excellent and almost unique match between the PDC-E2 autoepitope and a sequence in mycobacterial hsp65s, we tested the corresponding peptides for cross-reactivity using sera from 90 Spanish and British PBC patients. PMID: 15120760
  11. This study report two unrelated patients with pyruvate dehydrogenase deficiency caused by defects in the E2 subunit. PMID: 16049940
  12. epitope specificity of these PDC-E2 autoantibodies was distinctive suggesting that the mechanisms leading to loss of tolerance in the transplantation patients are distinct from primary biliary cirrhosis PMID: 17068145
  13. Species specificity in the interaction between hE1beta and hE2 in pyruvate dehydrogenase complex. PMID: 18206651
  14. tissue specificity of the autoimmune injury in primary biliary cirrhosis is a consequence of the unique characteristics of HIBECs during apoptosis and can be explained by exposure to immune system of intact immunoreactive PDC-E2 within apoptotic blebs. PMID: 19185000

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Involvement in disease
Pyruvate dehydrogenase E2 deficiency (PDHE2 deficiency)
Subcellular Location
Mitochondrion matrix.
Protein Families
2-oxoacid dehydrogenase family
Database Links

HGNC: 2896

OMIM: 245348

KEGG: hsa:1737

STRING: 9606.ENSP00000280346

UniGene: Hs.335551

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