DLAT Antibody

Datasheet
Code CSB-PA006926LA01HU
See More Details Free Antibody trial simple
Size US$299Purchase it in Cusabio online store
(only available for customers from the US)
Image
  • Western Blot
    Positive WB detected in: HepG2 whole cell lysate
    All lanes: DLAT antibody at 4μg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 69 kDa
    Observed band size: 69 kDa

  • Immunoprecipitating DLAT in HepG2 whole cell lysate
    Lane 1: Rabbit control IgG instead of CSB-PA006926LA01HU in HepG2 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
    Lane 2: CSB-PA006926LA01HU (6μg) + HepG2 whole cell lysate (1mg)
    Lane 3: HepG2 whole cell lysate (20μg)

Product Details

Full Product Name Rabbit anti-Homo sapiens (Human) DLAT Polyclonal antibody
Uniprot No. P10515
Target Names DLAT
Alternative Names Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial (EC 2.3.1.12) (70 kDa mitochondrial autoantigen of primary biliary cirrhosis) (PBC) (Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex) (M2 antigen complex 70 kDa subunit) (Pyruvate dehydrogenase complex component E2) (PDC-E2) (PDCE2), DLAT, DLTA
Raised in Rabbit
Species Reactivity Human
Immunogen Recombinant Human Dihydrolipoyllysine-residue acetyltransferase component of pyruvate dehydrogenase complex, mitochondrial protein (26-217AA)
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method >95%, Protein G purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form Liquid
Tested Applications ELISA, WB, IP
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IP 1:200-1:2000
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunoprecipitation (IP) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
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Citations

Potentially Critical Roles of NDUFB5, TIMMDC1, and VDAC3 in the Progression of Septic Cardiomyopathy Through Integrated Bioinformatics Analysis. Kang K, et al,Dna and Cell Biology,2019

Sample type: Heart tissue
Sample species: Mouse
Application:WB
Review: Expression of hub genes was measured by qRT- PCR and Western blotting. Scale bar: 20mm (A); mean–SEM value of at least six mice per group.
PMID: 31794266

Target Data

Function The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and thereby links the glycolytic pathway to the tricarboxylic cycle.
Involvement in disease Pyruvate dehydrogenase E2 deficiency (PDHE2 deficiency)
Subcellular Location Mitochondrion matrix
Protein Families 2-oxoacid dehydrogenase family
Database Links

HGNC: 2896

OMIM: 245348

KEGG: hsa:1737

STRING: 9606.ENSP00000280346

UniGene: Hs.335551

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