Recombinant Human Disheveled-associated activator of morphogenesis 1 (DAAM1), partial

Code CSB-YP896514HU
MSDS
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Source Yeast
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Code CSB-EP896514HU
MSDS
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Source E.coli
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Code CSB-EP896514HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP896514HU
MSDS
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Source Baculovirus
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Code CSB-MP896514HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
DAAM1
Uniprot No.
Alternative Names
DAAM1; KIAA0666; Disheveled-associated activator of morphogenesis 1
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Binds to disheveled (Dvl) and Rho, and mediates Wnt-induced Dvl-Rho complex formation. May play a role as a scaffolding protein to recruit Rho-GDP and Rho-GEF, thereby enhancing Rho-GTP formation. Can direct nucleation and elongation of new actin filaments. Involved in building functional cilia. Involved in the organization of the subapical actin network in multiciliated epithelial cells. Together with DAAM2, required for myocardial maturation and sarcomere assembly.
Gene References into Functions
  1. coiling phagocytosis is a mechanism for borrelial internalization by neuroglial cells mediated by Daam1 PMID: 29746581
  2. Results provide evidence that Daam1 activates RhoA to regulate Wnt5ainduced glioblastoma cell invasion. PMID: 29207169
  3. DAAM1 organizes actin filaments into a nodal complex PMID: 27760153
  4. Kank1 plays a crucial role in regulating the activity of RhoA through retrieving excess Daam1 and balancing the activities of RhoA and its effectors. PMID: 28284839
  5. High Daam-1 expression may upregulate the Wnt/PCP pathway and cause idiopathic pulmonary arterial hypertension PMID: 28288669
  6. Kif26b, together with Dvl3/Daam1, initiates cell polarity through the control of planar cell polarity-signaling pathway-dependent activation in endothelial cells. PMID: 26792835
  7. Daam1 plays a dual role in the phagocytic uptake of borreliae. PMID: 24696301
  8. A developmentally restricted function of miR-490 and its putative DAAM1 target are associated with exaggerated megakaryocytopoiesis and/or proplatelet formation. PMID: 22869791
  9. Wnt5a promotes breast cancer cell migration via Dvl2/Daam1/RhoA. PMID: 22655072
  10. DAAM1 is a formin required for centrosome re-orientation during cell migration. PMID: 20927366
  11. Fli-I promotes the GTP-bound active Rho-mediated relief of the autoinhibition of Daam1 and mDia1. Thus, Fli-I is a novel positive regulator of Rho-induced linear actin assembly mediated by DRFs. PMID: 20223827
  12. DAAM1 regulates endothelial cell growth through MT stabilization in a cell type-selective manner PMID: 20351293
  13. DAAM1 was shown to bind to the SH3 domain of CIP4 in vivo. PMID: 16630611
  14. Results report that Profilin1 is an effector downstream of Daam1 required for cytoskeletal changes during gastrulation. PMID: 17021034
  15. The DAAM1 FH2 domain structure, determined at 2.25 A resolution, and DAAM1 actin binding activity PMID: 17482208
  16. Crystal structure of human DAAM1 formin homology 2 domain PMID: 17986009
  17. a carboxyl-terminal binding partner, Dvl, has a role in activation of Daam1 PMID: 18162551
  18. mDia1 and Daam1 are platelet actin assembly factors having distinct efficiencies, and they are directly regulated by Rho GTPases PMID: 18218625

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Subcellular Location
Cytoplasm. Cytoplasm, cytoskeleton, cilium basal body. Note=Perinuclear.
Protein Families
Formin homology family
Tissue Specificity
Expressed in all tissues examined.
Database Links

HGNC: 18142

OMIM: 606626

KEGG: hsa:23002

STRING: 9606.ENSP00000247170

UniGene: Hs.19156

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