Recombinant Human E3 ubiquitin-protein ligase DTX3L (DTX3L)

Code CSB-YP855054HU
MSDS
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Source Yeast
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Code CSB-EP855054HU
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Source E.coli
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Code CSB-EP855054HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP855054HU
MSDS
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Source Baculovirus
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Code CSB-MP855054HU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
DTX3L
Uniprot No.
Alternative Names
B lymphoma and BAL associated protein; B-lymphoma- and BAL-associated protein; BBAP; Deltex 3 like; DTX 3L; DTX3L; DTX3L_HUMAN; E3 ubiquitin-protein ligase DTX3L; Protein deltex 3 like; Protein deltex 3 like protein; Protein deltex-3-like; Rhysin 2; Rhysin-2; Rhysin2
Species
Homo sapiens (Human)
Expression Region
2-740
Target Protein Sequence
ASHLRPPSP LLVRVYKSGP RVRRKLESYF QSSKSSGGGE CTVSTQEHEA PGTFRVEFSE RAAKERVLKK GEHQILVDEK PVPIFLVPTE NSIKKNTRPQ ISSLTQSQAE TPSGDMHQHE GHIPNAVDSC LQKIFLTVTA DLNCNLFSKE QRAYITTLCP SIRKMEGHDG IEKVCGDFQD IERIHQFLSE QFLESEQKQQ FSPSMTERKP LSQQERDSCI SPSEPETKAE QKSNYFEVPL PYFEYFKYIC PDKINSIEKR FGVNIEIQES SPNMVCLDFT SSRSGDLEAA RESFASEFQK NTEPLKQECV SLADSKQANK FKQELNHQFT KLLIKEKGGE LTLLGTQDDI SAAKQKISEA FVKIPVKLFA ANYMMNVIEV DSAHYKLLET ELLQEISEIE KRYDICSKVS EKGQKTCILF ESKDRQVDLS VHAYASFIDA FQHASCQLMR EVLLLKSLGK ERKHLHQTKF ADDFRKRHPN VHFVLNQESM TLTGLPNHLA KAKQYVLKGG GMSSLAGKKL KEGHETPMDI DSDDSKAASP PLKGSVSSEA SELDKKEKGI CVICMDTISN KKVLPKCKHE FCAPCINKAM SYKPICPTCQ TSYGIQKGNQ PEGSMVFTVS RDSLPGYESF GTIVITYSMK AGIQTEEHPN PGKRYPGIQR TAYLPDNKEG RKVLKLLYRA FDQKLIFTVG YSRVLGVSDV ITWNDIHHKT SRFGGPEMYG YPDPSYLKRV KEELKAKGIE
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
E3 ubiquitin-protein ligase which, in association with ADP-ribosyltransferase PARP9, plays a role in DNA damage repair and in interferon-mediated antiviral responses. Monoubiquitinates several histones, including histone H2A, H2B, H3 and H4. In response to DNA damage, mediates monoubiquitination of 'Lys-91' of histone H4 (H4K91ub1). The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 'Lys-20' methylation (H4K20me). PARP1-dependent PARP9-DTX3L-mediated ubiquitination promotes the rapid and specific recruitment of 53BP1/TP53BP1, UIMC1/RAP80, and BRCA1 to DNA damage sites. By monoubiquitinating histone H2B H2BC9/H2BJ and thereby promoting chromatin remodeling, positively regulates STAT1-dependent interferon-stimulated gene transcription and thus STAT1-mediated control of viral replication. Independently of its catalytic activity, promotes the sorting of chemokine receptor CXCR4 from early endosome to lysosome following CXCL12 stimulation by reducing E3 ligase ITCH activity and thus ITCH-mediated ubiquitination of endosomal sorting complex required for transport ESCRT-0 components HGS and STAM. In addition, required for the recruitment of HGS and STAM to early endosomes. In association with PARP9, plays a role in antiviral responses by mediating 'Lys-48'-linked ubiquitination of encephalomyocarditis virus (EMCV) and human rhinovirus (HRV) C3 proteases and thus promoting their proteosomal-mediated degradation.
Gene References into Functions
  1. Immunohistochemical analysis demonstrated that DTX3L was highly expressed in the glioma tissues and its level was correlated with the grade of malignancy. Multivariate analysis revealed the association between high expression of DTX3L and the poor prognosis of glioma patients. In addition, knockdown of DTX3L by siRNA transfection increased glioma cell apoptosis. PMID: 28627634
  2. we also found that DTX3L expression was regulated by focal adhesion kinase. Taken together, the results of this study show that DTX3L plays an important role in the proliferation and cell adhesion-mediated drug resistance of multiple myeloma cells and as such may play a key role in the development of multiple myeloma. PMID: 28653881
  3. A novel role for the really interesting new gene-domain E3 ubiquitin ligase deltex-3-like (DTX3L) in regulating CXCR4 sorting from endosomes to lysosomes. PMID: 24790097
  4. we report the high-resolution crystal structure of this previously uncharacterized C-terminal domain of human DTX3L, which we term the Deltex C-terminal domain. PMID: 22411408
  5. Overexpression of DTX3L, PIK3R4, ATP2C1, and SLC25A36, all located at 3q21.1-23 are associated with cervical cancer. PMID: 18618715

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Subcellular Location
Cytoplasm. Nucleus. Early endosome membrane; Peripheral membrane protein; Cytoplasmic side. Lysosome membrane; Peripheral membrane protein; Cytoplasmic side.
Protein Families
Deltex family
Database Links

HGNC: 30323

OMIM: 613143

KEGG: hsa:151636

STRING: 9606.ENSP00000296161

UniGene: Hs.518201

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