Recombinant Human E3 ubiquitin-protein ligase HUWE1 (HUWE1), partial

1. Ubiquitination modification through the coordinated action of PAQR3 with HUWE1 plays a crucial role in regulating the activity of hepatic PPARalpha in response to starvation. PMID: 29331071
2. HUWE1 missense variants may cause syndromic ID in both males and females. PMID: 29180823
3. HUWE1 overexpression could functionally suppress prostate carcinoma development both in vitro and in vivo, possibly by inverse regulation of c-Myc. PMID: 29966975
4. RBBP7 controls the stability of HUWE1.RBBP7 promotes HUWE1 ubiquitination. PMID: 29738775
5. The data supports an indispensable role for Mule in cardiac homeostasis through the regulation of mitochondrial function via maintenance of Pgc-1alpha and Pink1 expression and persistent negative regulation of c-Myc. PMID: 28148912
6. these results suggest that HUWE1 protein could contribute to preimplantation embryo development and dysregulated expression of HUWE1 could be related to poor embryo development and miscarriage in IVF clinic. PMID: 27901130
7. The authors show that HUWE1 dimerizes in solution and self-associates in cells, and that both occurs through the crystallographic dimer interface. They also demonstrate that HUWE1 is inhibited in cells and that it can be activated by disruption of the dimer interface. PMID: 28193319
8. High HUWE1 expression is associated with Ovarian cancer. PMID: 28687618
9. HUWE1 is essential for genomic stability, by promoting replication of damaged DNA We show that HUWE1-knockout cells are unable to mitigate replication stress, resulting in replication defects and DNA breakage. PMID: 27146073
10. N-myc expression was upregulated in response to stress or following loss of Huwe1, which led to increased proliferation and stem-cell exhaustion. PMID: 27668798
11. The interaction with Huwe1 and polyubiquitylation were blocked by disruption of casein kinase 1 (CK1) activity, and mass spectrometry and mutational analysis identified serine 334 as an important phosphorylation site for Atoh1 ubiquitylation and subsequent degradation. PMID: 27542412
12. Elevated HECTH9 protein expression is associated with pathogenesis of hepatocellular carcinoma. PMID: 28205209
13. Mechanistic investigation revealed that HUWE1 can regulate p53 protein level through its stabilization. HUWE1 functions as a tumour suppressor in thyroid cancer progression, which may represent a novel therapeutic target for prevention or intervention of thyroid cancer. PMID: 27581931
14. We identified a HUWE1 mutation in an affected male with Juberg-Marsidi and Brooks syndromes from the original family reported by Juberg and Marsidi; it is evident the syndrome does not result from a mutation in ATRX as reported in the literature. Additionally, the data indicated that Juberg-Marsidi syndrome and Brooks syndromes are allelic having the same HUWE1 mutation. PMID: 27130160
15. Results reveal a pathway controlled by ATM, SIRT6, and SNF2H to block HUWE1, which stabilizes H2AX and induces its incorporation into chromatin only when cells are damaged. PMID: 26711340
16. miR-542-5p plays a critical role in the proliferation of osteosarcoma and targets HUWE1. PMID: 26498360
17. TNF activates Mule by inducing the dissociation of Mule from its inhibitor ARF. Inhibition of Mule phosphorylation by silencing Syk prevents this, thereby inhibiting Mule E3 ligase activity and TNF-induced JNK activation and cell death. PMID: 26212014
18. HUWE1 and NEDD4-1 are two E3 ligases that are fundamental enzymes in the post-translational regulation of ABCG1 and ABCG4 protein levels and cellular cholesterol export activity PMID: 26296893
19. Results show that ARF-BP1 was expressed at high levels in B-cell lymphoma cell lines and by regulating MYC and p53 transcriptional activity, ARF-BP1 is a critical determinant of the proliferation of B cell lymphomas. PMID: 22754359
20. Our findings highlight the importance of microduplications at Xp11.22 to ID, even in sporadic cases, and reveal new clinical and molecular insight into HUWE1 copy number gains. PMID: 25652354
21. we show that HUWE1 stimulates human lung cancer cell invasion through regulating TIAM1 stability. Finally, we demonstrate that HUWE1 and TIAM1 protein levels are inversely correlated in human lung carcinomas PMID: 25543140
22. Inhibition of HUWE1 stabilizes MIZ1 and induces global accumulation of MIZ1 on MYC-bound target genes. PMID: 25253726
23. analysis of ubiquitin-mediated proteolysis of DNA damage-inducible transcript 4 (DDIT4) by the E3 ligase HUWE1 PMID: 25147182
24. Results demonstrate that HUWE1 is a novel player involved in regulating ERK1/2 signal transmission through the Shoc2 scaffold complex. PMID: 25022756
25. Ubiquitin ligase HUWE1 regulates axon branching through the Wnt/beta-catenin pathway in a Drosophila model for intellectual disability. PMID: 24303071
26. Low Huwe1 expression is associated with medulloblastoma. PMID: 24960692
27. this study shows that MutYH is ubiquitinated in vitro and in vivo by the E3 ligase Mule between amino acids 475 and 535. PMID: 24443563
28. data indicate that HUWE1 is a critical negative regulator of BRCA1 and suggest a new molecular mechanism for breast cancer pathogenesis PMID: 24342616
29. HUWE1 promotes BRCA1 ubiquitination. PMID: 24472556
30. Findings indicate p53-independent roles for MDM2 and HUWE1 in apoptosis and suggest the potential for therapy directed against MDM2 to overcome lapatinib resistance. PMID: 23652204
31. a critical regulatory mechanism of HDAC2 by Mule and suggest this pathway determines the cellular response to HDACs and DNA damage PMID: 22016339
32. HUWE1 and TRIP12 collaborate in degradation of ubiquitin-fusion proteins and misframed ubiquitin. PMID: 23209776
33. Increased dosage of HUWE1 causes nonsyndromic intellectual disability. PMID: 22840365
34. In PE, MULE preferentially targeted p53 for degradation, allowing accumulation of pro-apoptotic Mcl-1 isoforms. In IUGR, however, MULE targeted pro-survival Mcl-1, allowing p53 to accumulate and exert its apoptotic function. PMID: 22552282
35. the involvement of HUWE1 in the ubiquitination and proteasomal degradation of MyoD was described. PMID: 22277673
36. The E3 ligase Mule mediates the degradation of Pol lambda and the control of Pol lambda levels by Mule has functional consequences for the ability of mammalian cells to deal with 8-oxo-G lesions. PMID: 22203964
37. The present study reveals a novel regulation of RASSF1C and the potentially important role of RASSF1C in DNA damage responses via SCFbeta-TrCP and Mule. PMID: 21910689
38. Mitochondrion-dependent N-terminal processing of outer membrane Mcl-1 protein removes an essential Mule/Lasu1 protein-binding site. PMID: 21613222
39. Studies indicate that ARF is induced in response to DNA damage and inhibits, by direct interaction, the E3 ubiquitin ligase Mule. PMID: 21726556
40. findings suggest that reduced Mule/Mcl-1 complex has a significant role in increasing the stability of Mcl-1 in breast cancer cells and increased resistance to apoptosis PMID: 21730980
41. Huwe1 interacted with HIV-1 Gag-Pol precursor protein through an IN domain PMID: 21167302
42. Arf-bp1 and Pam are novel regulators of circadian gene expression that target Rev-erb alpha for degradation PMID: 20534529
43. A structural element within the HUWE1 HECT domain modulates self-ubiquitination and substrate ubiquitination activities. PMID: 20007713
44. study modifies the current view of ARF-mediated p53 activation and reveals that ARF-BP1 is a critical mediator of both the p53-independent and p53-dependent tumor suppressor functions of ARF PMID: 15989956
45. Mule is both required and sufficient for the polyubiquitination of Mcl-1; Mule is a unique BH3-containing E3 ubiquitin ligase apical to Bcl-2 family proteins during DNA damage-induced apoptosis PMID: 15989957
46. LASU1 is an E3 ligase that ubiquitinates Mcl-1 in vitro and is required for its proteasome-dependent degradation, the BH3 domain of LASU1 interactes with Mcl-1. PMID: 16213503
47. HectH9-mediated ubiquitination of Myc is required for transactivation of multiple target genes. PMID: 16269333
48. These findings demonstrate an important and conserved role for Huwe1 in regulating Cdc6 abundance after DNA damage. PMID: 17567951
49. An increased gene dosage of HSD17B10, HUWE1, or both contribute to the etiology of X-Linked Mental Retardation and suggest that point mutations in HUWE1 are associated with this disease too. PMID: 18252223
50. Huwe1 links destruction of N-Myc to the quiescent state that complements differentiation in the neural tissue PMID: 18488021

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HGNC: 30892

OMIM: 300697

KEGG: hsa:10075

STRING: 9606.ENSP00000262854

UniGene: Hs.136905