Recombinant Human E3 ubiquitin-protein ligase RNF6 (RNF6)

Code CSB-YP897080HU
MSDS
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Source Yeast
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Code CSB-EP897080HU
MSDS
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Source E.coli
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Code CSB-EP897080HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP897080HU
MSDS
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Source Baculovirus
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Code CSB-MP897080HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
RNF6
Uniprot No.
Alternative Names
E3 ubiquitin-protein ligase RNF6; Ring finger protein (C3H2C3 type) 6; Ring finger protein 6; RING H2 protein; RING H2 protein RNF6; RNF 6; RNF6; RNF6_HUMAN; SPG2
Species
Homo sapiens (Human)
Expression Region
1-685
Target Protein Sequence
MNQSRSRSDG GSEETLPQDH NHHENERRWQ QERLHREEAY YQFINELNDE DYRLMRDHNL LGTPGEITSE ELQQRLDGVK EQLASQPDLR DGTNYRDSEV PRESSHEDSL LEWLNTFRRT GNATRSGQNG NQTWRAVSRT NPNNGEFRFS LEIHVNHENR GFEIHGEDYT DIPLSDSNRD HTANRQQRST SPVARRTRSQ TSVNFNGSSS NIPRTRLASR GQNPAEGSFS TLGRLRNGIG GAAGIPRANA SRTNFSSHTN QSGGSELRQR EGQRFGAAHV WENGARSNVT VRNTNQRLEP IRLRSTSNSR SRSPIQRQSG TVYHNSQRES RPVQQTTRRS VRRRGRTRVF LEQDRERERR GTAYTPFSNS RLVSRITVEE GEESSRSSTA VRRHPTITLD LQVRRIRPGE NRDRDSIANR TRSRVGLAEN TVTIESNSGG FRRTISRLER SGIRTYVSTI TVPLRRISEN ELVEPSSVAL RSILRQIMTG FGELSSLMEA DSESELQRNG QHLPDMHSEL SNLGTDNNRS QHREGSSQDR QAQGDSTEMH GENETTQPHT RNSDSRGGRQ LRNPNNLVET GTLPILRLAH FFLLNESDDD DRIRGLTKEQ IDNLSTRHYE HNSIDSELGK ICSVCISDYV TGNKLRQLPC MHEFHIHCID RWLSENCTCP ICRQPVLGSN IANNG
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
E3 ubiquitin-protein ligase mediating 'Lys-48'-linked polyubiquitination of LIMK1 and its subsequent targeting to the proteasome for degradation. Negatively regulates axonal outgrowth through regulation of the LIMK1 turnover. Mediates 'Lys-6' and 'Lys-27'-linked polyubiquitination of AR/androgen receptor thereby modulating its transcriptional activity. May also bind DNA and function as a transcriptional regulator.
Gene References into Functions
  1. High RNF6 expression is associated with breast cancer. PMID: 28223545
  2. This study thus suggests that RNF6 overexpression in leukemia is under the direction of PBX1 and that the PBX1/RNF6 axis can be developed as a novel therapeutic target of leukemia. PMID: 26971355
  3. Rnf6 controls cellular LIMK1 concentrations and indicate a new function for the ubiquitin/proteasome system in regulating local growth cone actin dynamics PMID: 16204183
  4. Results describe thet cloning and identification of a novel RNF6 transcriptional splice variant, Spg2, in human development. PMID: 18368307
  5. Functional anaylysis of the mouse homolog PMID: 11971979

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Involvement in disease
Esophageal cancer (ESCR)
Subcellular Location
Nucleus. Cytoplasm. Cell projection, axon. Nucleus, PML body.
Protein Families
RNF12 family
Tissue Specificity
Weakly expressed in peripheral blood, spleen, prostate, testis and ovary. According to PubMed:18368307, it is preferentially expressed in testis and ovary and hardly detected in other tissues.
Database Links

HGNC: 10069

OMIM: 133239

KEGG: hsa:6049

STRING: 9606.ENSP00000342121

UniGene: Hs.136885

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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