Recombinant Human Electron transfer flavoprotein subunit beta (ETFB)

Code CSB-YP007845HU
MSDS
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Source Yeast
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Code CSB-EP007845HU
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Source E.coli
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Code CSB-EP007845HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP007845HU
MSDS
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Source Baculovirus
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Code CSB-MP007845HU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ETFB
Uniprot No.
Alternative Names
Beta ETF; Beta-ETF; Electron transfer flavoprotein beta polypeptide ; Electron transfer flavoprotein beta subunit ; Electron transfer flavoprotein subunit beta; Electron transferring flavoprotein beta polypeptide ; etfB; ETFB_HUMAN; FP585; MADD
Species
Homo sapiens (Human)
Expression Region
2-255
Target Protein Sequence
AELRVLVAV KRVIDYAVKI RVKPDRTGVV TDGVKHSMNP FCEIAVEEAV RLKEKKLVKE VIAVSCGPAQ CQETIRTALA MGADRGIHVE VPPAEAERLG PLQVARVLAK LAEKEKVDLV LLGKQAIDDD CNQTGQMTAG FLDWPQGTFA SQVTLEGDKL KVEREIDGGL ETLRLKLPAV VTADLRLNEP RYATLPNIMK AKKKKIEVIK PGDLGVDLTS KLSVISVEDP PQRTAGVKVE TTEDLVAKLK EIGRI
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Heterodimeric electron transfer flavoprotein that accepts electrons from several mitochondrial dehydrogenases, including acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (Probable). Required for normal mitochondrial fatty acid oxidation and normal amino acid metabolism. ETFB binds an AMP molecule that probably has a purely structural role.
Gene References into Functions
  1. We identified and replicated a novel gene, ETFB, strongly associated with chronic anthracycline-induced cardiotoxicity independently of age at tumor onset and related to anthracycline-mediated mitochondrial dysfunction PMID: 28913729
  2. These results suggested that increasing levels of ATP5B and ETFB were associated with worsening renal injury. PMID: 27840937
  3. the findings of our present study suggest that ETFbeta plays an important role in renal tubular cell apoptosis during the progression of DN. PMID: 27035869
  4. Electron Transfer Flavoprotein Subunit Beta Is a Candidate Endothelial Cell Autoantigen in Behcet's Disease PMID: 25915519
  5. Human METTL20 is a mitochondrial lysine methyltransferase that targets ETFbeta and modulates its activity. PMID: 25416781
  6. ETFB knockdown can affect TGF-beta-induced tissue remodeling and/or fibrotic processes in vitro. PMID: 23068445
  7. Point mutations in electron-transfer-flavoprotein beta is associated with destabilized conformations and defective protein:protein interactions leading to mitochondrial dysfunction. PMID: 22588007
  8. ETFB participates in the mechanoregulation of fibroblast cell number in collagen gel culture. PMID: 21903359
  9. non-productive conformation stabilization underpins rapid electron transfer to electron-transferring flavoprotein PMID: 15975918
  10. Tissue samples from 16 unrelated patients with ETF deficiency were analysed, two of them harboured mutations in the ETFB gene. PMID: 16510302
  11. conformational and stability properties of the disease-causing variant ETFbeta-D128N, and our findings on the effect of flavinylation in modulating protein conformational stability and activity. PMID: 19088074

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Involvement in disease
Glutaric aciduria 2B (GA2B)
Subcellular Location
Mitochondrion matrix.
Protein Families
ETF beta-subunit/FixA family
Tissue Specificity
Abundant in liver, heart and skeletal muscle. A weak expression is seen in the brain, placenta, lung, kidney and pancreas.
Database Links

HGNC: 3482

OMIM: 130410

KEGG: hsa:2109

STRING: 9606.ENSP00000346173

UniGene: Hs.348531

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