Recombinant Human Ephrin type-B receptor 2(EPHB2),partial

Code CSB-YP007730HU
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Source Yeast
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Code CSB-EP007730HU
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Source E.coli
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Code CSB-EP007730HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP007730HU
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Source Baculovirus
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Code CSB-MP007730HU
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names EPHB2
Uniprot No. P29323
Alternative Names cek5; Developmentally regulated EPH related tyrosine kinase; DRT; EK5; ELK related protein tyrosine kinase; Eph receptor B2; EPH tyrosine kinase 3; EPH-like kinase 5; EPHB2; EPHB2_HUMAN; Ephrin type B receptor 2; Ephrin type-B receptor 2; EPHT 3; ERK; ETECK; hEK5; Nuk; Prkm 5; Receptor protein tyrosine kinase HEK 5; Renal carcinoma antigen NY-REN-47; Sek 3; Tyro 5; Tyrosine protein kinase receptor CEK 5; Tyrosine protein kinase receptor EPH 3; Tyrosine protein kinase receptor QEK 5; Tyrosine-protein kinase receptor EPH-3; Tyrosine-protein kinase TYRO5
Species Homo sapiens (Human)
Protein Length Partial
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
(From Uniprot)
Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Functions in axon guidance during development. Involved in the guidance of commissural axons, that form a major interhemispheric connection between the 2 temporal lobes of the cerebral cortex. Also involved in guidance of contralateral inner ear efferent growth cones at the midline and of retinal ganglion cell axons to the optic disk. In addition to axon guidance, also regulates dendritic spines development and maturation and stimulates the formation of excitatory synapses. Upon activation by EFNB1, abolishes the ARHGEF15-mediated negative regulation on excitatory synapse formation. Controls other aspects of development including angiogenesis, palate development and in inner ear development through regulation of endolymph production. Forward and reverse signaling through the EFNB2/EPHB2 complex regulate movement and adhesion of cells that tubularize the urethra and septate the cloaca. May function as a tumor suppressor.
Gene References into Functions
  1. These findings suggested that miR-204 might serve as a tumor suppressor in the development of cervical cancer by directly targeting EphB2. PMID: 28800788
  2. EphB2 signaling-mediated Sirt3 expression reduces MSC senescence by maintaining mitochondrial reactive oxygen species homeostasis. PMID: 28687409
  3. This is the first study to link SLC1A3 and EPHB2 to clinically relevant vertebral osteoporosis phenotypes. PMID: 27476799
  4. Data show that EPHB2 predicted poor breast cancer survival and EPHB2 protein expression has also prognostic value depending on cell localization. PMID: 26870995
  5. showed that patients with SSc or SLE have AAb against EphB2, a protein involved in angiogenesis, and THEX1, a 3'-5' exoribonuclease involved in histone mRNA degradation. We have further identified a peptide from EphB2 as a specific and sensitive tool for SLE diagnosis PMID: 27617966
  6. show that expression of EPHB2 and SNAIL1 - an inducer of epithelial-mesenchymal transition (EMT) - is anti-correlated in colorectal cancer cell lines and tumors PMID: 27504909
  7. Tiam2/Rac are key components of EphB2 trans-endocytosis and are important for cell repulsion. PMID: 27597758
  8. High expression of junctional adhesion molecule-A and EphB2 can predict poor overall survival and high mortality rate, and EphB2 is an independent prognostic biomarker in lung adenocarcinoma patients. PMID: 28231727
  9. Data show that activation of EphB2 receptor kinase arrests tau protein hyperphosphorylation through phosphatidylinositol 3-kinase (PI3K)/Akt protein-mediated glycogen synthase kinase-3beta (GSK-3beta) inhibition. PMID: 26119563
  10. Expression of the Receptor Tyrosine Kinase EphB2 on Dendritic Cells Is Modulated by Toll-Like Receptor Ligation but Is Not Required for T Cell Activation PMID: 26407069
  11. Myosin 1 functions as an effector of EphB2/ephrinB signaling, controls cell morphology, and thereby cell repulsion. PMID: 26195670
  12. EphB2 activation is required for ependymoma development as well as it inhibits differentiation and promotes proliferation of the transformed cell. PMID: 25801123
  13. The results show an intricate interplay between p53 and TGF-beta3 whereby p53 inhibits the TGF-beta3-induced expression of genes, e.g., EPHB2, to impede tumor cell invasion and migration PMID: 25257729
  14. loss of EphB2 and gain of EphB4 expression represents an inflection point in the development, growth and possibly progression of TCC. PMID: 25148033
  15. knockdown impairs monocyte transmigration through the endothelium PMID: 24522257
  16. the upregulation of EphB2 receptors and its specific ligands leads to cholangiocarcinoma metastasis. PMID: 25012246
  17. Serrated colorectal carcinoma as proposed to arise from serrated adenoma is characterized by down regulation of EphB2. PMID: 24612059
  18. Comparative crystalisation analysis of the human EphB family kinases has yielded new crystal forms of the human EphB2 and EphB4 catalytic domains. PMID: 24677421
  19. EphB2 was involved in human naive B cell activation through Src-p65 and Notch1 signaling pathways and could be regulated by miR-185. PMID: 24803541
  20. Overexpression of EphB2 is associated with glioma. PMID: 23835497
  21. EphB2 plays an important role in cervical cancer progression by orchestrating an epithelial-mesenchymal transition program through R-Ras activation. PMID: 24439224
  22. A mechanism involving a triple role for EphB2 in breast cancer progression, whereby it regulates apoptosis, autophagy, and invasion. PMID: 24211352
  23. overexpression of Ephrin B2 receptor is associated with malignant mesothelioma. PMID: 23887168
  24. Mutation of EPHB2 is frequent in colorectal tumors with microsatellite instability. PMID: 24222164
  25. Marrow stromal cell subpopulations express EphB2 engrafted in the fetal intestinal epithelium when xenografted into sheep PMID: 23413357
  26. EphB2 silencing increased tumor cell proliferation. PMID: 22310282
  27. High ephrin-B1 expression is associated with medulloblastoma. PMID: 22723427
  28. PTPN14 has a role in angiogenesis and/or arteriovenous fate, acting via EphrinB2 and ACVRL1/activin receptor-like kinase 1 PMID: 22233626
  29. Data indicated for the first time that EphrinB2 and EphB4 expressions increase according to the histopathological grade and KPS score of glioma, and their expression levels are related to the progression-free survival of glioblastoma patients. PMID: 22374425
  30. Define the transcriptional targets of the reverse signaling by EphB2 acting exclusively as a ligand in epidermal keratinocyte differentiation. PMID: 21809346
  31. High Eph-B2 is associated with Waldenstrom's macroglobulinemia. PMID: 22010211
  32. EphB2 is an ephrin receptor and is up-regulated in invasive tumors but its role needs to be confirmed in further cases of Wilms tumors. PMID: 21387540
  33. EPHB2 interacts with EPHB6 in breast tumor cell lines PMID: 21737611
  34. data suggest that genetic variation at the EphB2 locus may increase risk of sporadic prostate cancer risk in African American men. PMID: 21603658
  35. Mutation in EphB2 gene is associated with colorectal adenocarcinoma PMID: 21161727
  36. at least in the context of pancreatic carcinoma CFPAC-1 cells, EphB2 plays a tumor suppressor role in cell proliferation and apoptosis PMID: 21292437
  37. Even a moderate level of EphB2 expression has effects on tumour cells which results in reduced migration and invasiveness and slows the growth of colonic tumour implants in an in vivo model. PMID: 20339854
  38. The MAPK pathway is important in the pro-death action of EphB2, through ERK1/2 phosphorylation and inhibition of this pathway using PD98059 counters EphB2-driven cell death. PMID: 20046096
  39. RYK, a catalytically inactive receptor tyrosine kinase, associates with EphB2 and EphB3 but does not interact with AF-6. PMID: 11956217
  40. role of extracellular signal-regulated kinase (ERK) during the differentiation of human monoblastic U937 cells stimulated by granulocyte-macrophage colony-stimulating factor (GM-CSF) and tumor necrosis factor (TNF) PMID: 12063024
  41. liganded to EFNB1 and EFNB2, expresssed in gastric cancer PMID: 12136247
  42. Glioma migration and invasion are promoted by activation of EphB2 or inhibited by blocking EphB2. Dysregulation of EphB2 expression or function may underlie glioma invasion. PMID: 15126357
  43. our findings suggest that mutational inactivation of EPHB2 may be important in the progression and metastasis of prostate cancer. PMID: 15300251
  44. loss of EphB expression represents a critical step in colorectal cancer progression PMID: 15973414
  45. Deregulated EphB2 expression may play a role in several cancer types with loss of EphB2 expression serving as an indicator of the possible pathogenetic role of EphB2 signaling in the maintenance of tissue architecture of colon epithelium. PMID: 16166419
  46. progressive loss of EphB2 expression seen in each critical step of colon carcinogenesis, including the onset of invasion, dedifferentiation and metastasis which are paralleled by adverse patient outcome PMID: 16272170
  47. These results demonstrate that the MAPK ERK signaling pathway contributes to the p53-independent antiproliferative functions of p14ARF. Furthermore, they identify a new mechanism by which phosphorylation at serine 216 participates to Cdc25C inactivation. PMID: 16582626
  48. analysis of germline EPHB2 alterations in patients with colorectal tumors PMID: 16740153
  49. High EPHB2 mutation rate is associated with gastric tumors with microsatellite instability PMID: 16819508
  50. novel link between EphB forward signaling and SDF-1-induced signaling demonstrates a mechanism for cooperative regulation of endothelial cell movement. PMID: 16840724

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Involvement in disease Prostate cancer (PC)
Subcellular Location Cell membrane, Single-pass type I membrane protein, Cell projection, axon, Cell projection, dendrite
Protein Families Protein kinase superfamily, Tyr protein kinase family, Ephrin receptor subfamily
Tissue Specificity Brain, heart, lung, kidney, placenta, pancreas, liver and skeletal muscle. Preferentially expressed in fetal brain.
Database Links

HGNC: 3393

OMIM: 176807

KEGG: hsa:2048

STRING: 9606.ENSP00000363763

UniGene: Hs.380705

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