Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

HSD17B1

Uniprot No.

P14061

Research Area

Cancer

Alternative Names

(17-beta-HSD 1)(20 alpha-hydroxysteroid dehydrogenase)(20-alpha-HSD)(E2DH)(Estradiol 17-beta-dehydrogenase 1)(Placental 17-beta-hydroxysteroid dehydrogenase)(Short chain dehydrogenase/reductase family 28C member 1)

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

2-328aa

Target Protein Sequence

ARTVVLITGCSSGIGLHLAVRLASDPSQSFKVYATLRDLKTQGRLWEAARALACPPGSLETLQLDVRDSKSVAAARERVTEGRVDVLVCNAGLGLLGPLEALGEDAVASVLDVNVVGTVRMLQAFLPDMKRRGSGRVLVTGSVGGLMGLPFNDVYCASKFALEGLCESLAVLLLPFGVHLSLIECGPVHTAFMEKVLGSPEEVLDRTDIHTFHRFYQYLAHSKQVFREAAQNPEEVAEVFLTALRAPKPTLRYFTTERFLPLLRMRLDDPSGSNYVTAMHREVFGDVPAKAEAGAEAGGGAGPGAEDEAGRGAVGDPELGDPPAAPQ
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

42.3 kDa

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal 10xHis-tagged and C-terminal Myc-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The recombinant Human HSD17B1 protein production is achieved through the manipulation of desired gene expression in e.coli cells. The sequence (2-328aa) of foreign DNA is fused to an expression vector and then transformed into e.coli cells. The positive cells are selected and cultured to induce the expression of the desired protein. A N-terminal 10xHis tag and C-terminal Myc tag is fused to the protein. The recombinant Human HSD17B1 protein is subjected to affinity purification. Its purity is over 85%, as measured by SDS-PAGE.

Target Background

Function

Favors the reduction of estrogens and androgens. Converts estrone (E1) to a more potent estrogen, 17beta-estradiol (E2). Also has 20-alpha-HSD activity. Uses preferentially NADH.

Gene References into Functions

Our genetic study demonstrated HSD17B1 937 G variant as a risk factor for infertility in women with stage I and II endometriosis in Polish Caucasian patients. PMID: 28405865
HSD17B1 is a promising marker to predict EC prognosis. PMID: 27923582
Macronutrient intakes were associated with serum estradiol level, and these associations may be modified by HSD17B1 polymorphism in postmenopausal Japanese women. PMID: 28980890
the effect of 17beta-HSD1 expression on breast cancer cell transcript profile in steroid-deprived condition, was investigated. PMID: 27544780
Mutation in the 17-beta-hydroxysteroid dehydrogenase gene is associated with congenital adrenal hyperplasia. PMID: 28207417
Substrate inhibition of 17beta-HSD1 in tumor epithelial cells and regulation of 17beta-HSD7 by 17beta-HSD1 knockdown has been demonstrated. PMID: 28554725
10 natural products were evaluated leading to inhibition of the activity of 17beta-HSD1 at the concentration of 25 muM, and six of them inhibit the activity over 50%. PMID: 27933483
The structure of human 17beta-HSD1 and biological function have been studied extensively in the last two decades. In humans, the enzyme is expressed in placenta, ovary, endometrium and breast; high activity of estrogen activation provides the basis of 17beta-HSD1's implication in estrogen-dependent diseases, such as breast cancer, endometriosis and non-small cell lung carcinomas. [Review] PMID: 27102893
These findings suggested that the HSD17B1 Ser312Gly polymorphism might confer genetic cancer susceptibility in an ethnic-dependent manner, especially among Caucasians. PMID: 26923943
The rs605059 polymorphism of the HSD17B1 gene is associated with increased risk of recurrent spontaneous abortion. PMID: 25394609
Review/Meta-analysis: HSD17B1 rs605059 polymorphisms were not associated with the risks of endometrial cancer or endometriosis. PMID: 26261478
the HSD17B1 transcript and protein were identified in HeLa, SiHa, Ca Ski and C-33A cervical cancer cells. PMID: 26054693
Increased expression of HSD17B1 in NSCLC may contribute to an elevated intratissue level of E2 and consequently may support the development and spread of cancer. PMID: 25564397
The key enzymes of ovarian steroidogenesis, CYP 19 and 17beta-HSD, are active in the ovaries of postmenopausal women. PMID: 24855493
17beta-HSD1 and 17beta-HSD7 are principal reductive 17beta-hydroxysteroid dehydrogenases and major players in the viability of estrogen-dependent breast cancer cells. PMID: 25257817
E1-induced ERELuc activity in the inhibitor-treated uterus was reduced by the HSD17B1 inhibitor. PMID: 25617485
Single nucleotide polymorphism in HSD17B1 gene is associated with hepatocellular carcinoma. PMID: 24563232
Onset of epileptiform discharges is modestly delayed in slices from ovariectomized rats replaced with physiologically relevant doses of EB, but the number of discharges is not affected. PMID: 24113171
results demonstrated that HSD17B1 expression and its ability to convert the weak estrogen E1 to the more potent E2 can be associated with DNA methylation in the 5' flanking region in GC cells. PMID: 23916544
analysis of intratumoral localization and activity of 17beta-hydroxysteroid dehydrogenase type 1 in non-small cell lung cancer PMID: 23837683
We found the presence of HSD17B1 transcripts and proteins in human Jurkat acute T cell leukemia, Hut-78 and Raji B lymphoma cells PMID: 23540283
HSD17B1-mediated decreased conversion of estrone to estradiol may reduce the estrogenic effects, thereby reducing the risk of developing breast cancer during long-term hormone replacement therapy use. [Meta-analysis] PMID: 23430226
study demonstrated that 17beta-HSD1 increases breast cancer cell migration, in spite of its positive regulation of the antimetastatic gene NM23 PMID: 22691413
In this review, the results suggest that HSD17B1 and PR polymorphism may play a role in the risk of endometriosis PMID: 22613790
Frequencies of rs605059 AA genotype and A allele were significantly increased in Chinese patients with uterine leiomyoma compared to healthy controls. PMID: 22546946
Data suggest that HSD17B1 is overexpressed in endometrial cancer tissue; HSD17B1 appears responsible for shift toward reductive metabolism (i.e., greater reduction of estrone to 17-beta-estradiol; lesser oxidation of 17-beta-estradiol to estrone). PMID: 22362820
Breast cancer cells T47D, MCF-7, BT 20, and JEG 3 as control cells, were chosen to evaluate the contribution of 17beta-hydroxysteroid dehydrogenase type 1 and type 2 to the estradiol/estrone ratio. PMID: 22253796
reduced HSD17B1 expression can be associated with DNA methylation in the 5' flanking region of HSD17B1 in CRC from the proximal colon. PMID: 22176788
HSD17B1 gene may contribute to sex steroid-mediated effects on colorectal cancer development. PMID: 21317201
SNPs (HSD17B1 (rs2010750, rs598126 and rs676387), COMT (rs4680), UGT1A1 (rs8175347) and ESR1 (rs9340799)) seem to be related to mammographic density in the same direction of their associations with breast cancer risk PMID: 22199302
Data show that in 17beta-HSD1 inhibitors are placed in the substrate-binding site and they occupy an apolar subpocket consisting of the following amino acids: Gly94, Leu95, Leu96, Asn152, Tyr155, and Phe192. PMID: 21857977
HSD17B1 upregulation by sodium butyrate is associated with increased conversion of E1 to E2 in colorectal cancer cells. PMID: 21563966
Obese women with selected CYP19 and 17-beta HSD gene variants had remarkably different E2 trajectories around the the final menstrual period, resulting in different postmenopausal E2 levels. PMID: 21198743
Genetic variants in ESR1, ESR2, and HSD17B1 genes could modify susceptibility to endometriosis and might influence the fertility status in endometriosis patients. PMID: 20586553
This study suggests that HSD17B1 312Gly allele may be a protective factor for breast cancer development in Caucasians. PMID: 20151320
CYP17 (T1931C) and HSD17beta1 (A1954G) polymorphisms may jointly increase the risk of breast cancer. PMID: 20193327
genetic assiciation studies [Japan, Brazil]: data suggest SNP in 17beta-HSD1 gene may modify association between dietary isoflavone intake & breast cancer PMID: 20432167
17beta-HSD1 up-regulates breast cancer cell growth by a dual action on estradiol synthesis and dihydrotestosterone inactivation. PMID: 20172961
A significant increment of 17beta-HSD1 followed exemestane neoadjuvant therapy of postmenopausal ER-positive breast carcinoma. This may represent the compensatory response of breast carcinoma tissues to estrogen depletion. PMID: 20151319
An amino acid important for steroid/retinoid discrimination was identified and its significance was highlighted by the results of molecular modeling studies. PMID: 20382160
Data report structures of the binary and ternary complexes of 17beta-HSD1 with a new inhibitor E2B, opening a new orientation of breast cancer treatment. PMID: 19929851
increased activity in hypothalamic obesity PMID: 12519880
possible hypothalamic mediators regulating adipose tissue 11 beta-HSD-1 might include down-regulation by CRH, ACTH, and alpha 2 sympathetic stimulation, and up-regulation by beta 2 sympathetic stimulation and by the cytokines TNFalpha and IL-1 beta PMID: 12519881
examination of amplification in breast cancer PMID: 12527905
Evidence for association between the Ser312Gly polymorphism in HSD17B1 and endometriosis was found in a Japanese population PMID: 15640252
the germline variants in HSD17B1 characterized by htSNPs do not substantially influence the risk of prostate cancer in U.S. and European whites PMID: 16311626
Corticotrophin-releasing hormone (CRH) increased whereas alpha-helical CRH decreased the mRNA levels of STS, CYP19A1, and HSD17B1, the key enzymes for estrogen synthesis. PMID: 16467490
HSD17B1 is a multispecific enzyme. PMID: 16480815
importance of 17HSD type 2 in breast cancer- & non-tumour breast cell lines; high or low expression affected oestradiol concentration significantly; response dependent on endogenous expression of 17HSD type 1 which seems to be dominant to 17HSD type 2 PMID: 16954436
These results suggest that soy consumption may interact with polymorphisms in the 17beta-HSD1 gene in relation to endometrial cancer risk. PMID: 17301695

Hide All

Subcellular Location

Cytoplasm.

Protein Families

Short-chain dehydrogenases/reductases (SDR) family

Database Links

HGNC: 5210

OMIM: 109684

KEGG: hsa:3292

STRING: 9606.ENSP00000466799

UniGene: Hs.654385

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Code	CSB-EP010766HU
Abbreviation	Recombinant Human HSD17B1 protein
MSDS	MSDS Datasheet
Size	$256
	Order now
Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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