Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

GSPT1

Uniprot No.

P15170

Research Area

Epigenetics and Nuclear Signaling

Alternative Names

GSPT1; ERF3AEukaryotic peptide chain release factor GTP-binding subunit ERF3A; Eukaryotic peptide chain release factor subunit 3a; eRF3a; G1 to S phase transition protein 1 homolog

Species

Homo sapiens (Human) parvovirus B19 (isolate AU)

Source

Baculovirus

Expression Region

1-499aa

Target Protein Sequence

MELSEPIVENGETEMSPEESWEHKEEISEAEPGGGSLGDGRPPEESAHEMMEEEEEIPKPKSVVAPPGAPKKEHVNVVFIGHVDAGKSTIGGQIMYLTGMVDKRTLEKYEREAKEKNRETWYLSWALDTNQEERDKGKTVEVGRAYFETEKKHFTILDAPGHKSFVPNMIGGASQADLAVLVISARKGEFETGFEKGGQTREHAMLAKTAGVKHLIVLINKMDDPTVNWSNERYEECKEKLVPFLKKVGFNPKKDIHFMPCSGLTGANLKEQSDFCPWYIGLPFIPYLDNLPNFNRSVDGPIRLPIVDKYKDMGTVVLGKLESGSICKGQQLVMMPNKHNVEVLGILSDDVETDTVAPGENLKIRLKGIEEEEILPGFILCDPNNLCHSGRTFDAQIVIIEHKSIICPGYNAVLHIHTCIEEVEITALICLVDKKSGEKSKTRPRFVKQDQVCIARLRTAGTICLETFKDFPQMGRFTLRDEGKTIAIGKVLKLVPEKD
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

99.8 kDa

Protein Length

Full Length

Tag Info

N-terminal MBP-tagged and C-terminal 6xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The recombinant Human GSPT1 was expressed with the amino acid range of 1-499. This GSPT1 protein is theoretically predicted to have a molecular weight of 99.8 kDa. Expression of this GSPT1 protein is conducted in baculovirus. The N-terminal MBP tag and C-terminal 6xHis tag was fused into the coding gene segment of GSPT1, making it easier to detect and purify the GSPT1 recombinant protein in the later stages of expression and purification.

The current research on Eukaryotic peptide chain release factor GTP-binding subunit ERF3A (GSPT1) focuses on two major areas: protein synthesis and transcriptional regulation. In the realm of protein synthesis, GSPT1's role is closely scrutinized, particularly its potential impact on regulating the growth and survival of tumor cells. On the other hand, in the field of transcriptional regulation, GSPT1 participates in intricate networks that govern gene expression, influencing cellular physiological processes. These two primary research directions aim to provide new perspectives for a deeper understanding of GSPT1 functions and the treatment of associated diseases.

Target Background

Function

Involved in translation termination in response to the termination codons UAA, UAG and UGA. Stimulates the activity of ETF1. Involved in regulation of mammalian cell growth. Component of the transient SURF complex which recruits UPF1 to stalled ribosomes in the context of nonsense-mediated decay (NMD) of mRNAs containing premature stop codons. Required for SHFL-mediated translation termination which inhibits programmed ribosomal frameshifting (-1PRF) of mRNA from viruses and cellular genes.

Gene References into Functions

PABP enhances the productive binding of the eRF1-eRF3 complex to the ribosome, via interactions with the N-terminal domain of eRF3a which itself has an active role in translation termination. PMID: 27418677
Data found that the N-terminal glycine repeat of eRF3a influences eRF3a-PABP interaction and that eRF3a 12-GGC allele has a decreased binding affinity for PABP. PMID: 26818177
GAB2, GSPT1, TFDP2 and ZFPM1 are four new susceptibility loci for testicular germ cell tumor. PMID: 26503584
The survivin and eRF3 complex may function in spindle formation and segregation of chromosomes and cytokinesis. PMID: 23377885
Overexpression of ID1 in two different cell lines induced STMN3 and GSPT1 at the transcriptional level, while depletion of ID1 reduced their expression. PMID: 25028095
The proteolytic cleavage of eRF3a and eRF3b into p-eRF3 leads to release an amino-terminal fragment containing nuclear export signal to allow the relocalization of eRF3 into the nucleus to interact with the p14ARF. PMID: 24569073
ERF3 is targeted for caspase-mediated proteolytic cleavage and degradation during DNA damage-induced apoptosis. PMID: 23054082
Our results show that the presence of the longer allele of eRF3a is correlated with threefold increased risk of breast cancer development PMID: 22101789
A biological role for the overlapping ERF3 PAM2 motifs in the regulation of deadenylase accessibility to PABPC1 at the 3' end of poly(A). PMID: 23019593
crystal structures of the MLLE domain from PABPC1 in complex with the two PAM2 regions of eRF3 PMID: 20418951
GSPT1/eRF3 is proteolytically processed into an IAP-binding protein PMID: 12865429
Human translation termination factor eRF3/GSPT1 is an interacting partner of RNase L. PMID: 15908960
Polyglycine expansions in eukaryotic translation release factor 3 are associated with gastric cancer susceptibility PMID: 15987717
eRF3a controls the formation of the termination complex by modulating eRF1 protein stability. PMID: 15987998
Further, we describe a novel complex that contains the NMD factors SMG-1 and Upf1, and the translation termination release factors eRF1 and eRF3 (SURF). an association between SURF and the EJC is required for SMG-1-mediated Upf1 phosphorylation and NMD. PMID: 16452507
GSTP1 inhibits the binding of MAP kinase kinase kinase 5 (MAP3K5) to the 14-3-3 protein, a MAP3K5 inhibitor, while a novel role of GSPT1 is revealed in the regulation of apoptosis signal-regulating kinase 1 (ASK1)-mediated apoptosis. PMID: 17700517
eRF3a is degraded by the proteasome when not associated with eRF1; proteasomal degradation of eRF3a controls translation termination complex formation by adjusting the eRF3a level to that of eRF1. PMID: 18083835
eRF3a/GSPT1 12-GGC allele increases the susceptibility for breast cancer development. PMID: 19424636
GSPT1 GGC(12) allele was present in 2.2% of colorectal cancer patients but was absent in Crohn disease patients and in the control group PMID: 19963113

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Protein Families

TRAFAC class translation factor GTPase superfamily, Classic translation factor GTPase family, ERF3 subfamily

Database Links

HGNC: 4621

OMIM: 139259

KEGG: hsa:2935

STRING: 9606.ENSP00000398131

UniGene: Hs.528780

Code	CSB-BP009966HU
Abbreviation	Recombinant Human GSPT1 protein
MSDS	MSDS Datasheet
Size	$528
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Based on the SEQUEST from database of Baculovirus host and target protein, the LC-MS/MS Analysis result of CSB-BP009966HU could indicate that this peptide derived from Baculovirus-expressed Homo sapiens (Human) parvovirus B19 (isolate AU) GSPT1. Based on the SEQUEST from database of Baculovirus host and target protein, the LC-MS/MS Analysis result of CSB-BP009966HU could indicate that this peptide derived from Baculovirus-expressed Homo sapiens (Human) parvovirus B19 (isolate AU) GSPT1.
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Recombinant Human Eukaryotic peptide chain release factor GTP-binding subunit ERF3A (GSPT1)

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