Recombinant Human F-box/WD repeat-containing protein 1A(BTRC)

Code CSB-YP897464HU
Size Pls inquire
Source Yeast
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-EP897464HU
Size Pls inquire
Source E.coli
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-EP897464HU-B
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-BP897464HU
Size Pls inquire
Source Baculovirus
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-MP897464HU
Size Pls inquire
Source Mammalian cell
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Purity >85% (SDS-PAGE)
Target Names BTRC
Uniprot No. Q9Y297
Alternative Names Beta transducin repeat containing E3 ubiquitin protein ligase; beta TrCP1; beta-transducin repeat containing ; BETA-TRCP; betaTrCP; btrC; bTrCP; bTrCP1; E3RSIkappaB; F box and WD repeat protein 1B; F box and WD repeats protein beta TrCP; F-box and WD repeats protein beta-TrCP; F-box/WD repeat protein 1A; F-box/WD repeat-containing protein 1A; FBW1A; FBW1A_HUMAN; FBXW1; FBXW1A; FWD1; HOS; mKIAA4123; pIkappaBalpha-E3 receptor subunit; Slimb
Species Homo sapiens (Human)
Expression Region 1-605
Target Protein Sequence MDPAEAVLQE KALKFMCSMP RSLWLGCSSL ADSMPSLRCL YNPGTGALTA FQNSSEREDC NNGEPPRKII PEKNSLRQTY NSCARLCLNQ ETVCLASTAM KTENCVAKTK LANGTSSMIV PKQRKLSASY EKEKELCVKY FEQWSESDQV EFVEHLISQM CHYQHGHINS YLKPMLQRDF ITALPARGLD HIAENILSYL DAKSLCAAEL VCKEWYRVTS DGMLWKKLIE RMVRTDSLWR GLAERRGWGQ YLFKNKPPDG NAPPNSFYRA LYPKIIQDIE TIESNWRCGR HSLQRIHCRS ETSKGVYCLQ YDDQKIVSGL RDNTIKIWDK NTLECKRILT GHTGSVLCLQ YDERVIITGS SDSTVRVWDV NTGEMLNTLI HHCEAVLHLR FNNGMMVTCS KDRSIAVWDM ASPTDITLRR VLVGHRAAVN VVDFDDKYIV SASGDRTIKV WNTSTCEFVR TLNGHKRGIA CLQYRDRLVV SGSSDNTIRL WDIECGACLR VLEGHEELVR CIRFDNKRIV SGAYDGKIKV WDLVAALDPR APAGTLCLRT LVEHSGRVFR LQFDEFQIVS SSHDDTILIW DFLNDPAAQA EPPRSPSRTY TYISR
Protein Length full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Earn $30 Amazon Card or 20μL/μg CUSABIO Trial Size Antibody. Details of rewards >>

Target Data

Function Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Recognizes and binds to phosphorylated target proteins
Gene References into Functions
  1. The primary role of the S(52,56) residues of Vpu in antagonism of CD4, GaLV Env, and BST-2/tetherin is to recruit the SCF/betaTrCP ubiquitin ligase. PMID: 30347660
  2. TSPAN15 interacts with BTRC to promote oesophageal squamous cell carcinoma metastasis via activating NF-kappaB signaling. PMID: 29650964
  3. BTrCP-FBXW2-SKP2 axis forms an oncogene-tumour suppressor-oncogene cascade to control cancer cell growth with FBXW2 acting as a tumour suppressor by promoting SKP2 degradation. PMID: 28090088
  4. Data show that the SCF(beta-TrCP) ubiquitin E3 ligase complex responsible for regulating the active protein kinase C-associated kinase (RIPK4) level. PMID: 29435596
  5. SAG/RBX2 E3 ligase complexes with UBCH10 and UBE2S ubiquitin-conjugating enzymes to ubiquitylate beta-TrCP1 via K11-linkage for degradation. PMID: 27910872
  6. Data indicate the role of tyrosine kinase c-Src (Src) in rescuing Taz (transcriptional coactivator with PDZ-binding motif) from E3 ligase SCF(beta-TrCP)-mediated degradation. PMID: 28154141
  7. SCFbeta-TRCP negatively regulates the FLCN complex by promoting FNIP2 degradation in Birt-Hogg-Dube syndrome-associated renal cancer. PMID: 28039480
  8. beta-TRCP depletion in HepG2 hepatocellular carcinoma cells resulted in increased Lipin1 protein abundance. PMID: 28049764
  9. It demonstrates that degradation of Lipin-1 is regulated by BTRC in the cytoplasm and on membranes PMID: 28483528
  10. It has been proposed that CENP-W may function as a booster of beta-TrCP1 nuclear import to increase the oncogenicity of beta-TrCP1. PMID: 27861801
  11. oncogenic effect of miR-182 and its reversal by beta-TrCP2 were confirmed in vivo This study suggests that beta-TrCP and miR-182 may be possible biomarkers and targets for early detection and treatment of pancreatic cancer PMID: 27797718
  12. Beta-TrCP controls ubiquitination and degradation of liver-enriched transcription factor CREB-H. PMID: 27029215
  13. Phosphorylated VEGFR-2 was reduced by hyperglycemia while total VEGFR-2 was almost unaltered. However, VEGFR-2 was reduced when directly exposed to reactive oxygen species, with resultant co-location of beta-TrCP and VEGFR-2. PMID: 26935904
  14. EBV-miR-BART10-3p facilitated epithelial-mesenchymal transition and promotes metastasis of nasopharyngeal carcinoma by targeting BTRC. PMID: 26497204
  15. beta-TrCP1/FWD1 dominant negative mutant has a role in reducing myeloma cell growth PMID: 26009993
  16. This study showed SCF(beta-TRCP) earmarks Set8 for ubiquitination and degradation in a casein kinase I-dependent manner, which is activated by DNA-damaging agents. PMID: 26666832
  17. SCF(beta-TrCP1/2) interacting partners, were identified. PMID: 25900982
  18. a model that CD166 regulates MCAM through a signaling flow from activation of PI3K/AKT and c-Raf/MEK/ERK signaling to the inhibition of potential MCAM ubiquitin E3 ligases, betaTrCP and Smurf1. PMID: 26004137
  19. Human rotavirus NSP1 functions by mimicking the host IkappaB phosphodegron recognized by beta-TrCP. PMID: 25626907
  20. Interaction between the phosphorylated degron motif of TRIM9 and the WD40 repeat region of beta-TrCP prevented beta-TrCP from binding its substrates, stabilizing IkappaBalpha and p100 and thereby blocking NF-kappaB activation. PMID: 25190485
  21. These results show that human rotaviruses commonly inhibit the NF-kappaB pathway via their NSP1 protein by degrading beta-TrCP and thus stabilizing IkappaB. PMID: 25701827
  22. No evidence of a direct association between beta-TRCP polymorphisms and breast cancer. PMID: 26003292
  23. identified 221 proteins that contained the DpSGXX(X)pS motif and also interacted specifically with the WD40 repeats of betaTrCP. PMID: 25515538
  24. beta-TrCP has an important role in controlling H3K27 trimethylation activity and lymphoma pathogenesis by targeting EZH2 for degradation. PMID: 24469040
  25. TRIB2 associated-ubiquitin E3 ligases beta-transducin repeat-containing E3 ubiquitin protein ligase (beta-TrCP), COP1 and Smad ubiquitination regulatory factor 1 (Smurf1) reduced TCF4/beta-Catenin expression, and these effects could be enhanced by TRIB2. PMID: 25311538
  26. NORE1A has a role in Ras regulation of SCF(beta-TrCP) protein activity and specificity PMID: 25217643
  27. Study provides a novel molecular mechanism for the negative regulation of MTSS1 by beta-TRCP in cancer cells. PMID: 24318128
  28. Upon DNA-damaging anticancer drug treatment, CK1/beta-TrCP-mediated Tiam1 degradation is abolished, and the accumulated Tiam1 contributes to downstream activation of Rac1/JNK. PMID: 24737324
  29. Wnt stimulation induced prolonged monoubiquitination of Gsk3beta and Gsk3beta-beta-TrCP interaction. PMID: 24451375
  30. betaTrCP-dependent degradation of TFAP4 is required for the fidelity of mitotic division PMID: 24500709
  31. this study showed betaTrCP-mediated regulation of Mis18beta stability is a mechanism to restrict centromere function of Mis18 complex from late mitosis to early G1 phase. PMID: 24269809
  32. we found no evidence of association between the 9N ins/del polymorphism of the b-TrCP gene and EOC in this Chinese population PMID: 23546975
  33. SIRT1 acts as a negative regulator of beta-TrCP synthesis via promoting protein degradation. PMID: 24211209
  34. a model in which TNFalpha-induced beta-TrCP1 accumulation promotes SMRT degradation and the subsequent induction of proinflammatory gene expression. PMID: 23861398
  35. suggest that interaction of S100A4 and Rhotekin permits S100A4 to complex with RhoA and switch Rho function from stress fiber formation to membrane ruffling to confer an invasive phenotype PMID: 22964642
  36. SGT1 could enhance the binding between PHLPP1 and beta-TrCP which has been documented to be able to target PHLPP1 for destruction PMID: 23440515
  37. Results indicate that beta-TrCP1 is involved in the negative regulation of TRF1. PMID: 23583392
  38. Hsp27 and F-box protein beta-TrCP promote degradation of mRNA decay factor AUF1. PMID: 23530064
  39. mutational analysis indicates that IKKalpha-dependent phosphorylation at Ser-304 is crucial to the binding of HuR to beta-TrCP1. PMID: 23115237
  40. Knockdown of beta-TrCP1 and beta-TrCP2 attenuated the K48-linked ubiquitination and degradation of IRAK1. PMID: 22851693
  41. Fas-associated factor 1 is a scaffold protein that promotes beta-transducin repeat-containing protein (beta-TrCP)-mediated beta-catenin ubiquitination and degradation. PMID: 22730322
  42. Identified are three arginine residues of beta TrCP that participate in NRF2 docking. PMID: 22751928
  43. phosphorylated TAZ binds to the beta-TrCP subunit of the SCF(beta-TrCP) E3 ubiquitin ligase PMID: 22692215
  44. Data show that beta-TRCP knockdown causes accumulation of VEGFR2, resulting in increased activity of signaling pathways downstream of VEGFR2. PMID: 22711876
  45. this review discusses beta-TrCP's new downstream substrate, DEPTOR, as well as summarize the novel functional aspects of beta-TrCP in controlling cell growth and regulating autophagy, in part through governing the stability of DEPTOR.[Review] PMID: 22454292
  46. IL-10R1 is a novel substrate of betaTrCP-containing ubiquitin E3 ligase, a novel negative regulatory mechanism that may potentially affect IL-10 function PMID: 22087322
  47. Data indicate that knockdown of beta-TrCP1 and beta-TrCP2 markedly reduced IL-17-induced, phosphorylation-dependent ubiquitination and degradation of Act1. PMID: 22045853
  48. mTOR drives its own activation via SCF(betaTrCP)-dependent degradation of the mTOR inhibitor DEP PMID: 22017875
  49. Endogenous DEPTOR levels are negatively regulated by betaTrCP. PMID: 22017876
  50. mTOR generates an auto-amplification loop by triggering the betaTrCP- and CK1alpha-dependent degradation of DEPTOR PMID: 22017877

Show More

Hide All

Subcellular Location Cytoplasm, Nucleus
Tissue Specificity Expressed in epididymis (at protein level).
Database Links

HGNC: 1144

OMIM: 603482

KEGG: hsa:8945

STRING: 9606.ENSP00000359206

UniGene: Hs.643802

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

© 2007-2021 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1