Recombinant Human Fibroblast growth factor receptor 4(FGFR4),partial

Code CSB-YP008648HU1
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Source Yeast
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Code CSB-EP008648HU1
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Source E.coli
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Code CSB-EP008648HU1-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP008648HU1
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Source Baculovirus
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Code CSB-MP008648HU1
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names FGFR4
Uniprot No. P22455
Alternative Names CD 334; CD334; CD334 antigen; fc13h 10; fc13h10; Fgfr 4; FGFR-4; Fgfr4; FGFR4_HUMAN; Fibroblast growth factor receptor 4; Hydroxyaryl protein kinase; JTK 2; JTK2; MGC20292; Protein tyrosine kinase; TKF; Tyrosine kinase related to fibroblast growth factor receptor; Tyrosylprotein kinase
Species Homo sapiens (Human)
Protein Length Partial
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
(From Uniprot)
Tyrosine-protein kinase that acts as cell-surface receptor for fibroblast growth factors and plays a role in the regulation of cell proliferation, differentiation and migration, and in regulation of lipid metabolism, bile acid biosynthesis, glucose uptake, vitamin D metabolism and phosphate homeostasis. Required for normal down-regulation of the expression of CYP7A1, the rate-limiting enzyme in bile acid synthesis, in response to FGF19. Phosphorylates PLCG1 and FRS2. Ligand binding leads to the activation of several signaling cascades. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. Phosphorylation of FRS2 triggers recruitment of GRB2, GAB1, PIK3R1 and SOS1, and mediates activation of RAS, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling pathway, as well as of the AKT1 signaling pathway. Promotes SRC-dependent phosphorylation of the matrix protease MMP14 and its lysosomal degradation. FGFR4 signaling is down-regulated by receptor internalization and degradation; MMP14 promotes internalization and degradation of FGFR4. Mutations that lead to constitutive kinase activation or impair normal FGFR4 inactivation lead to aberrant signaling.
Gene References into Functions
  1. The FGFR4-388arg variant promotes lung cancer progression by N-cadherin induction. PMID: 29402970
  2. These findings suggest that high levels of FGFR4 can increase glucose metabolism and lead to chemoresistance in breast cancer and reveal the mechanistic basis for targeting FGFR4 as a therapeutic opportunity for chemoresistant tumors. PMID: 29763898
  3. Taken together, this study provides the first evidence that Blu9931 functions as a FGFR4-selective inhibitor in colorectal cancer (CRC) cells, and Blu9931 may be a new targeted drug. PMID: 29048661
  4. Results show that FGFR4 promotes gastric cancer cell proliferation, migration and epithelial-to-mesenchymal transition. Its expression is negatively regulated by miR-491-5p through SNAIL protein. PMID: 29569792
  5. Our findings indicate that the FGFR4-388Arg variant may play an important role in lung squamous cell carcinoma, which may be mediated by the overactivation of the MAPK pathway. PMID: 28583379
  6. FGFR4 rs351855 could be a novel independent prognostic factor of BCR after radical prostatectomy in the Chinese population. PMID: 27640814
  7. This meta-analysis demonstrates the FGFR rs351855 G>A polymorphism is associated with increased cancer risk. PMID: 28445975
  8. Our results indicate that mRNA expression of FGFR4-related genes may be a biomarker to define the distinctive molecular phenotype of intrahepatic cholangiocarcinoma PMID: 28445152
  9. The present study employed FGFR4 polymorphism to help identify treatments for high risk patients with stage III colon cancer. PMID: 27857023
  10. Our data indicate that FGFR4 polymorphic isoforms mediate signaling that yields mitochondrial therapeutic targets of relevance to the actions of different somatostatin analogs. PMID: 27966451
  11. In radiosensitive SW480 and DLD1 cells, enforced expression of FGFR4 stabilized RAD51 protein levels resulting in enhanced clearance of gamma-H2AX foci and increased cell survival in the mismatch repair (MMR)-proficient SW480 cells. PMID: 27650548
  12. FGFR4/FGF19 autocrine signaling mediates the survival of a subset of basal-like breast cancer cells PMID: 27192118
  13. Study shows that loss of FGFR4 expression led to a remarkable increase in sorafenib-induced ROS generation and apoptosis of hepatocellular carcinoma (HCC) cell lines, and demonstrates that hyperactivation of FGF19/FGFR4 signaling in HCC is one of the main mechanisms of sorafenib resistance. PMID: 28069043
  14. This is the first study to elucidate FGF19/FGFR4 signaling in favor of hepatocellular carcinoma cells developing from fatty liver PMID: 27447573
  15. High expression of FGFR4 is associated with hepatocellular carcinoma. PMID: 26498355
  16. Findings show that FGF19 provides a cytoprotective role against ER stress by activating a FGFR4-GSK3beta-Nrf2 signaling cascade, suggesting targeting this signaling node as a candidate therapeutic regimen for hepatocellular carcinoma (HCC) management. PMID: 28951455
  17. FGF23 augments pro-fibrotic signalling cascades in injury-primed renal fibroblasts via activation of FGFR4 PMID: 28919046
  18. functional clathrin-mediated endocytosis is required for proper FGFR4 signaling PMID: 27615514
  19. The frequency of the FGFR4 Arg388 (A) allele was significantly higher in hypertensive subjects (36%) than controls (24.3%). GA,AA, and GA+AA genotypes were significantly associated with risk of hypertension, even after adjusting for age, body mass index, and glucose. Gender stratification showed a significant association only in female subjects. Subjects with GA and AA genotypes showed higher diastolic blood pressure. PMID: 28650667
  20. Enhanced myocardial expression of FGFR4 is associated with left ventricular hypertrophy in chronic kidney disease. PMID: 26681731
  21. High FGFR4 expression was significantly associated with the depth of invasion, lymph-node metastasis, pathological stage, and distant metastasis or recurrent disease in gastric cancer. PMID: 28056982
  22. This study showed that Gly388Arg FGFR4 Polymorphism Is Not Predictive of Everolimus Efficacy in Well-Differentiated Digestive Neuroendocrine Tumors. PMID: 26335532
  23. Data suggest that knockdown of fibroblast growth factor receptor 4 (FGFR4) expression might prevent the growth of astric cancer (GC) in vivo. PMID: 26432329
  24. FGFR4 is overexpressed in oral squamous cell carcinoma (OSCC) and oropharyngeal squamous cell carcinoma (OPSCC) in the absence of gene amplification, and may serve as a potential predictive marker for FGFR4-directed targeted therapy in OSCC and OPSCC. PMID: 26551585
  25. Study shows that FGF19 can be secreted and promotes ovarian cancer progression such as proliferation and invasion by activating FGFR4. PMID: 26323668
  26. The FGFR4 single-nucleotide polymorphism rs376618 had borderline significant differences in Patients With Primary Bile Acid Diarrhea. PMID: 26856750
  27. Our study provides the first evidence of a role for FGFR4 in neuroblastoma PMID: 26840079
  28. KL methylation is a characteristic of many breast cancer cases . However, the resulting or associated perturbation in FGFR4 expression, similar to FGF19, could be utilized as a biomarker for poor prognosis PMID: 26152288
  29. The genetic polymorphism in FGFR4 rs351855 may be associated with the risk of HCC coupled with liver cirrhosis and may markedly increase the AFP level PMID: 25860955
  30. Using Fgfr4 single nucleotide polymorphism knock-in mice and transgenic mouse models for breast and lung cancers, we validate the enhanced STAT3 signalling induced by the FGFR4 Arg388-variant in vivo PMID: 26675719
  31. High tumor expression of FGFR4 protein is not an independent risk factor for gastric cancer initiation, but is a useful prognostic marker for GC patients when the tumor is relatively small, well differentiated, or in the early stages of invasion. PMID: 25684949
  32. review summarizes all aspects of FGFR4 physiology and pathophysiology relevant to cacer. PMID: 23944363
  33. FGFR4 is an important determinant of the aggressive phenotype of the hepatoma/hepatocarcinoma cell lines. PMID: 25031272
  34. The structural basis for dual FGFR and EGFR targeting by FIIN3 also is illustrated by crystal structures of FIIN-3 bound with FGFR4 V550L and EGFR L858R. PMID: 25349422
  35. Variants in genes involved in feedback regulation of bile acid synthesis (KLB, P=0.06 and FGFR4, P=0.09) were potentially associated with the irritable bowel syndrome-diarrhea subgroup with elevated serum C4. PMID: 25070056
  36. The FGFR4 transmembrane polymorphic variants can modulate cellular growth and sensitivity to glucocorticoid hormone negative feedback. PMID: 24625004
  37. High FGFR4 expression is associated with drug resistance in alveolar rhabdomyosarcoma. PMID: 24550147
  38. In a Chinese population, the FGFR-4 gene polymorphism rs351855G/A was associated with an increased risk for ischemic stroke but was not associated with the ischemic stroke severity or subtype PMID: 24239227
  39. FGFR4 silencing markedly reduces the migration and invasion capacity of colorectal cancer cell lines. PMID: 24410190
  40. Silencing of FGFR4 reduced cell viability in a panel of colon cancer cell lines and increased caspase-dependent apoptosis. PMID: 24503538
  41. The results suggest that proteins, post-translational modifications or mutations that alter structural flexibility of the TKB domain of Cbl-family proteins could regulate their binding to target phosphoproteins and thereby, affect PTK-mediated signalling. PMID: 22888118
  42. expression of TGF-beta1 and FGFR4 could make synergy on the occurrence and progression of HCC, and may be used as prognosis indicators for HCC patients PMID: 24324363
  43. The genotypes of G388R in FGFR4 gene were in Hardy-Weinberg equilibrium. The percentage of normal homozygotes GG was found to be (48.5%) in breast cancer, (51.8%) in lung cancer. Glycine/arginine was (36.9%) in breast cancer, (42.9%) in lung cancer. PMID: 24416997
  44. The single nucleotide polymorphism rs1966265 in FGF receptor 4 is a possible genetic key variant in alveolar diseases of preterm newborns. PMID: 24288432
  45. FGFR4 rs351855G/A polymorphism is associated with increased susceptibility to non-Hodgkin's lymphoma (NHL) and could be used as a marker for predicting the prognosis of the malignancy. PMID: 24248544
  46. expression of FGFR4 in intrahepatic cholangiocarcinomas, perihilar cholangiocarcinomas and distal cholangiocarcinomas by immunohistochemistry, and association of FGFR4 with clinicopathologic parameters and survival rate PMID: 24565842
  47. Inhibition of the activity of FGFR4 may be the main mechanisms of PD effect while 5-Fu reduced FGFR4 expression PMID: 24126887
  48. In-depth DNA sequencing identified additional genetic coding and noncoding variants in FGFR4 that are associated with fecal bile acids excretion or colonic transit in Irritable bowel syndrome-diarrhea PMID: 24200957
  49. The distribution of FGFR4 AA genotype and A-allele of rs351855G/A was significantly lower in NSCLC patients. PMID: 23524567
  50. Suggest that the FGFR4 Gly388Arg polymorphism is not a risk factor for gastric cancer initiation but that it is a useful prognostic marker for GC patients when the tumor is relatively small, well differentiated, or at an early clinical stage. PMID: 23901234

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Involvement in disease Prostate cancer (PC)
Subcellular Location Cell membrane, Single-pass type I membrane protein, Endosome, Endoplasmic reticulum
Protein Families Protein kinase superfamily, Tyr protein kinase family, Fibroblast growth factor receptor subfamily
Tissue Specificity Expressed in gastrointestinal epithelial cells, pancreas, and gastric and pancreatic cancer cell lines.
Database Links

HGNC: 3691

OMIM: 134935

KEGG: hsa:2264

STRING: 9606.ENSP00000292408

UniGene: Hs.165950

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