Recombinant Human Fidgetin-like protein 1 (FIGNL1)

Code CSB-YP757658HU
MSDS
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Source Yeast
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Code CSB-EP757658HU
MSDS
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Source E.coli
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Code CSB-EP757658HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP757658HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP757658HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
FIGNL1
Uniprot No.
Alternative Names
FIGNL1Fidgetin-like protein 1; EC 3.6.4.-
Species
Homo sapiens (Human)
Expression Region
1-674
Target Protein Sequence
MQTSSSRSVH LSEWQKNYFA ITSGICTGPK ADAYRAQILR IQYAWANSEI SQVCATKLFK KYAEKYSAII DSDNVESGLN NYAENILTLA GSQQTDSDKW QSGLSINNVF KMSSVQKMMQ AGKKFKDSLL EPALASVVIH KEATVFDLPK FSVCGSSQES DSLPNSAHDR DRTQDFPESN RLKLLQNAQP PMVTNTARTC PTFSAPVGES ATAKFHVTPL FGNVKKENHS SAKENIGLNV FLSNQSCFPA ACENPQRKSF YGSGTIDALS NPILNKACSK TEDNGPKEDS SLPTFKTAKE QLWVDQQKKY HQPQRASGSS YGGVKKSLGA SRSRGILGKF VPPIPKQDGG EQNGGMQCKP YGAGPTEPAH PVDERLKNLE PKMIELIMNE IMDHGPPVNW EDIAGVEFAK ATIKEIVVWP MLRPDIFTGL RGPPKGILLF GPPGTGKTLI GKCIASQSGA TFFSISASSL TSKWVGEGEK MVRALFAVAR CQQPAVIFID EIDSLLSQRG DGEHESSRRI KTEFLVQLDG ATTSSEDRIL VVGATNRPQE IDEAARRRLV KRLYIPLPEA SARKQIVINL MSKEQCCLSE EEIEQIVQQS DAFSGADMTQ LCREASLGPI RSLQTADIAT ITPDQVRPIA YIDFENAFRT VRPSVSPKDL ELYENWNKTF GCGK
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Involved in DNA double-strand break (DBS) repair via homologous recombination (HR). Recruited at DSB sites independently of BRCA2, RAD51 and RAD51 paralogs in a H2AX-dependent manner. May regulate osteoblast proliferation and differentiation. May play a role in the control of male meiosis dynamic.
Gene References into Functions
  1. FIGL-1 is a new centrosomal protein and inhibits ciliogenesis. PMID: 27384458
  2. Further research assessing the functions and mechanisms of FIGNL1, and other homologous recombination pathway genes may disclose unique pathological characteristics of SCLC, and help identify potential therapeutic targets and biomarkers PMID: 28260065
  3. RAD51-binding protein fidgetin-like 1 (FIGNL1) is identified; FIGNL1 specifically interacts with RAD51 through its conserved RAD51 binding domain. Cells depleted of FIGNL1 show defective homologous recombination repair. PMID: 23754376
Subcellular Location
Nucleus. Cytoplasm. Cytoplasm, perinuclear region. Note=Together with RAD51 and a subset of H2A histone proteins, redistributed in discrete nuclear DNA damage-induced foci after ionizing radiation (IR) treatment (PubMed:23754376).
Protein Families
AAA ATPase family
Database Links

HGNC: 13286

OMIM: 615383

KEGG: hsa:63979

STRING: 9606.ENSP00000349356

UniGene: Hs.137516

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