Recombinant Human Folylpolyglutamate synthase, mitochondrial (FPGS)

Code CSB-YP2371HU
MSDS
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Source Yeast
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Code CSB-EP2371HU
MSDS
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Source E.coli
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Code CSB-EP2371HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP2371HU
MSDS
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Source Baculovirus
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Code CSB-MP2371HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
FOLC
Uniprot No.
Alternative Names
Folylpolyglutamate synthase, mitochondrial; EC 6.3.2.17; Folylpoly-gamma-glutamate synthetase; FPGS; Tetrahydrofolylpolyglutamate synthase; Tetrahydrofolate synthase; FPGS
Species
Homo sapiens (Human)
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Catalyzes conversion of folates to polyglutamate derivatives allowing concentration of folate compounds in the cell and the intracellular retention of these cofactors, which are important substrates for most of the folate-dependent enzymes that are involved in one-carbon transfer reactions involved in purine, pyrimidine and amino acid synthesis. Unsubstituted reduced folates are the preferred substrates. Metabolizes methotrexate (MTX) to polyglutamates.
Gene References into Functions
  1. The association of aberrant folylpolyglutamate synthetase splicing with ex vivo methotrexate resistance and clinical outcome in childhood acute lymphoblastic leukemia. PMID: 27036162
  2. Low expression level of FPGS is associated with neuroendocrine lung tumors. PMID: 27064343
  3. Our results revealed a significant difference of overall survival between patients with AA genotype and GA + GG genotype (P<0.05). Our study demonstrated a correlation of the polymorphism of FPGS rs1544105 with MTX treatment efficacy, as well as with overall survival, suggesting it could be used as an effective approach in the prediction of MTX treatment efficacy PMID: 27987364
  4. These results indicate that elevated expressions of the tumor-related genes FPGS/GGH and VEGF are correlated with malignancy of thymic carcinoma and B3 thymoma tumors. PMID: 27387303
  5. 529 adults (n = 325 European-Americans, 204 Egyptians) on a stable warfarin dose were genotyped for GGCX rs12714145 and rs10654848, FPGS rs7856096, and STX1B rs4889606. PMID: 26751406
  6. data suggest that FPGS modulation affects global and promoter CpG DNA methylation and expression of several genes involved in important biological pathways PMID: 26895662
  7. FPGS and ABCB1 genetic variants can influence the outcome in RA patients receiving MTX monotherapy. PMID: 26652611
  8. Genetic polymorphisms in the FPGS coding region are significantly associated with increased Acute Lymphoblastic Leukemia risk. PMID: 26107232
  9. study found that genetic polymorphism of FPGS rs10760502A > G is associated with susceptibility to primary retroperitoneal liposasrcoma PMID: 25765001
  10. antifolate resistant leukemia cells harbor a heterozygous point mutation in exon12 of FPGS which disrupts FPGS activity by abolishing ATP binding, and alters the binding pattern of transcription factors to the genomic region of exon12. PMID: 25229333
  11. Genome-wide association studies identify 10 novel genetic loci as risk factors for methotrexate response in rheumatoid arthritis patients additionally to polymorphism in DHFR, FPGS and TYMS. PMID: 24583629
  12. mitochondrial FPGS is required because folate polyglutamates are not substrates for transport across the mitochondrial membrane PMID: 25164808
  13. Polymorphism of FPGS rs1544105 G>A is associated with response to therapy in acute lymphoblastic leukemia. PMID: 24908438
  14. An interaction term, between FPGS rs7033913 heterozygotes and GGH rs11988534 homozygotes for the minor allele, had a p-value <0.0001 and may contribute to methotrexate toxicity in rheumatoid arthritis. PMID: 24447348
  15. High Folylpoly-glutamate synthetase expression is associated with response to therapy in non-small-cell lung cancer. PMID: 22895141
  16. A novel missense mutation 502/490 T>C (L151/101P) in exon 5 of FPGS gene is firstly found in Chinese Han children, and the cytosolic and mitochondrial variants display allelic frequencies of 0.70 % and 0.47 % respectively. PMID: 22809608
  17. The mbr enhanced the expression of FPGS significantly and increased sensitivity of Jurkat cells to MTX efficiently. PMID: 22698741
  18. This is one of the first studies to assess FPGS and GGH genetic variants in relation to plasma homocysteine. PMID: 22018726
  19. The expression of mFPGS was different in A549 cells exposed to different methotrexate enantiomers. PMID: 21418912
  20. intratumor FPGS plays an important role in the efficacy of oral fluoropyrimidine plus LV therapy for colorectal cancer. PMID: 21380490
  21. FPGS gene is proposed as a novel causative gene for cleft lip. PMID: 21093159
  22. Data revealed that high FPGS gene expression, low GGH gene expression and low ABCC1 gene expression in CRC tissues were predictive factors for a high reduced folate level after LV administration. PMID: 19636555
  23. low folylpolyglutamate synthase expression is associated with development of aggressive metastatic behavior of the colorectal cancer PMID: 14676127
  24. Significantly higher expression levels of FPGS were found in mucosa expressing the splice variant DCC342. PMID: 16122883
  25. human mitochondrial FPGS is at least in part strongly associated with the inner mitochondrial membrane PMID: 16169100
  26. Investigation of the molecular regulatory mechanisms directing FPGS gene expression in B precursor-ALL and T-ALL cells. PMID: 16707018
  27. study identified FPGS polymorphisms; results indicate that two of the cytosolic nonsynonymous coding SNPs affected protein expression, in vitro substrate kinetics & folate & antifolate efficacy in cells expressing the polymorphisms PMID: 17875718
  28. Results indicate that hFPGS can catalyze the processive addition of approximately four glutamate residues to DDAH 4PteGlu 1. PMID: 18672898
  29. Aberrant splicing of folylpolyglutamate synthetase is associated with antifolate resistance in leukemia. PMID: 19131550

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Subcellular Location
[Isoform 1]: Mitochondrion inner membrane. Mitochondrion matrix.; [Isoform 2]: Cytoplasm.
Protein Families
Folylpolyglutamate synthase family
Database Links

HGNC: 3824

OMIM: 136510

KEGG: hsa:2356

STRING: 9606.ENSP00000362344

UniGene: Hs.335084

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