Product Details

Uniprot No.

Target Names

Alternative Names

Folylpolyglutamate synthase, mitochondrial (EC 6.3.2.17) (Folylpoly-gamma-glutamate synthetase) (FPGS) (Tetrahydrofolylpolyglutamate synthase) (Tetrahydrofolate synthase), FPGS

Species Reactivity

Human

Immunogen

A synthesized peptide derived from Human FPGS

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

30C11

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, FC

Recommended Dilution

Application	Recommended Dilution
FC	1:50-1:200

Protocols

ELISA Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Folylpolyglutamate synthase (FPGS) plays a central role in folate metabolism by catalyzing the addition of glutamate residues to folate and antifolate compounds. This enzymatic activity is essential for cellular retention of folates and directly influences the efficacy of antifolate chemotherapeutics such as methotrexate, making FPGS a compelling target for cancer metabolism research and drug response studies. Understanding FPGS expression and regulation provides valuable insights into one-carbon metabolism pathways that support nucleotide biosynthesis and cellular proliferation.

This recombinant monoclonal antibody, clone 30C11, offers the reproducibility and consistency that demanding research applications require. Generated against a synthetic peptide derived from human FPGS and produced using recombinant technology in rabbit host, this antibody delivers sequence-defined specificity with minimal lot-to-lot variation. The affinity-chromatography purification ensures high purity, supporting reliable and reproducible experimental outcomes across your studies.

Validation in flow cytometry demonstrates clear detection of FPGS in HepG2 human hepatocellular carcinoma cells, with optimal performance at dilutions between 1:50 and 1:200. The flow cytometry protocol employed fixation and permeabilization to access intracellular FPGS, yielding distinct signal separation from isotype control, confirming specific detection in this metabolically active cell line. The antibody is also validated for ELISA applications, providing flexibility for quantitative detection workflows.

Whether investigating folate-dependent metabolic pathways, exploring mechanisms of antifolate drug resistance, or characterizing FPGS expression in cancer models, this antibody supports research spanning metabolism and signal transduction. The unconjugated format allows pairing with your preferred secondary detection system for customized experimental design.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Catalyzes conversion of folates to polyglutamate derivatives allowing concentration of folate compounds in the cell and the intracellular retention of these cofactors, which are important substrates for most of the folate-dependent enzymes that are involved in one-carbon transfer reactions involved in purine, pyrimidine and amino acid synthesis. Unsubstituted reduced folates are the preferred substrates. Metabolizes methotrexate (MTX) to polyglutamates.

Gene References into Functions

The association of aberrant folylpolyglutamate synthetase splicing with ex vivo methotrexate resistance and clinical outcome in childhood acute lymphoblastic leukemia. PMID: 27036162
Low expression level of FPGS is associated with neuroendocrine lung tumors. PMID: 27064343
Our results revealed a significant difference of overall survival between patients with AA genotype and GA + GG genotype (P<0.05). Our study demonstrated a correlation of the polymorphism of FPGS rs1544105 with MTX treatment efficacy, as well as with overall survival, suggesting it could be used as an effective approach in the prediction of MTX treatment efficacy PMID: 27987364
These results indicate that elevated expressions of the tumor-related genes FPGS/GGH and VEGF are correlated with malignancy of thymic carcinoma and B3 thymoma tumors. PMID: 27387303
529 adults (n = 325 European-Americans, 204 Egyptians) on a stable warfarin dose were genotyped for GGCX rs12714145 and rs10654848, FPGS rs7856096, and STX1B rs4889606. PMID: 26751406
data suggest that FPGS modulation affects global and promoter CpG DNA methylation and expression of several genes involved in important biological pathways PMID: 26895662
FPGS and ABCB1 genetic variants can influence the outcome in RA patients receiving MTX monotherapy. PMID: 26652611
Genetic polymorphisms in the FPGS coding region are significantly associated with increased Acute Lymphoblastic Leukemia risk. PMID: 26107232
study found that genetic polymorphism of FPGS rs10760502A > G is associated with susceptibility to primary retroperitoneal liposasrcoma PMID: 25765001
antifolate resistant leukemia cells harbor a heterozygous point mutation in exon12 of FPGS which disrupts FPGS activity by abolishing ATP binding, and alters the binding pattern of transcription factors to the genomic region of exon12. PMID: 25229333
Genome-wide association studies identify 10 novel genetic loci as risk factors for methotrexate response in rheumatoid arthritis patients additionally to polymorphism in DHFR, FPGS and TYMS. PMID: 24583629
mitochondrial FPGS is required because folate polyglutamates are not substrates for transport across the mitochondrial membrane PMID: 25164808
Polymorphism of FPGS rs1544105 G>A is associated with response to therapy in acute lymphoblastic leukemia. PMID: 24908438
An interaction term, between FPGS rs7033913 heterozygotes and GGH rs11988534 homozygotes for the minor allele, had a p-value <0.0001 and may contribute to methotrexate toxicity in rheumatoid arthritis. PMID: 24447348
High Folylpoly-glutamate synthetase expression is associated with response to therapy in non-small-cell lung cancer. PMID: 22895141
A novel missense mutation 502/490 T>C (L151/101P) in exon 5 of FPGS gene is firstly found in Chinese Han children, and the cytosolic and mitochondrial variants display allelic frequencies of 0.70 % and 0.47 % respectively. PMID: 22809608
The mbr enhanced the expression of FPGS significantly and increased sensitivity of Jurkat cells to MTX efficiently. PMID: 22698741
This is one of the first studies to assess FPGS and GGH genetic variants in relation to plasma homocysteine. PMID: 22018726
The expression of mFPGS was different in A549 cells exposed to different methotrexate enantiomers. PMID: 21418912
intratumor FPGS plays an important role in the efficacy of oral fluoropyrimidine plus LV therapy for colorectal cancer. PMID: 21380490
FPGS gene is proposed as a novel causative gene for cleft lip. PMID: 21093159
Data revealed that high FPGS gene expression, low GGH gene expression and low ABCC1 gene expression in CRC tissues were predictive factors for a high reduced folate level after LV administration. PMID: 19636555
low folylpolyglutamate synthase expression is associated with development of aggressive metastatic behavior of the colorectal cancer PMID: 14676127
Significantly higher expression levels of FPGS were found in mucosa expressing the splice variant DCC342. PMID: 16122883
human mitochondrial FPGS is at least in part strongly associated with the inner mitochondrial membrane PMID: 16169100
Investigation of the molecular regulatory mechanisms directing FPGS gene expression in B precursor-ALL and T-ALL cells. PMID: 16707018
study identified FPGS polymorphisms; results indicate that two of the cytosolic nonsynonymous coding SNPs affected protein expression, in vitro substrate kinetics & folate & antifolate efficacy in cells expressing the polymorphisms PMID: 17875718
Results indicate that hFPGS can catalyze the processive addition of approximately four glutamate residues to DDAH 4PteGlu 1. PMID: 18672898
Aberrant splicing of folylpolyglutamate synthetase is associated with antifolate resistance in leukemia. PMID: 19131550

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Subcellular Location

[Isoform 1]: Mitochondrion inner membrane. Mitochondrion matrix.; [Isoform 2]: Cytoplasm.

Protein Families

Folylpolyglutamate synthase family

Database Links

HGNC: 3824

OMIM: 136510

KEGG: hsa:2356

STRING: 9606.ENSP00000362344

UniGene: Hs.335084

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Code	CSB-RA983477A0HU
Size	US$210
	Order now
Image	Overlay Peak curve showing HepG2 cells stained with CSB-RA983477A0HU (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1µg/110⁶cells) for 45min at 4℃. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG(H+L) at 1:200 dilution for 35min at 4℃.Control antibody (green line) was rabbit IgG (1µg/110⁶cells) used under the same conditions. Acquisition of >10,000 events was performed.
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FPGS Recombinant Monoclonal Antibody

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Target Background