Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

ALDOA

Uniprot No.

P04075

Research Area

Metabolism

Alternative Names

ALDA; Aldo1; ALDOA; ALDOA_HUMAN; Aldolase 1; Aldolase A; Aldolase A fructose bisphosphatase; Aldolase A fructose bisphosphate; Aldolase; fructose-bisphosphate A; Epididymis secretory sperm binding protein Li 87p; FRUCTOALDOLASE A; Fructose 1 6 bisphosphate triosephosphate lyase; Fructose bisphosphate aldolase A; Fructose bisphosphate aldolase; FRUCTOSE-1,6-BISPHOSPHATE ALDOLASE A; Fructose-bisphosphate aldolase A; Fructose-bisphosphate aldolase A Muscle-type; GSD12; HEL S 87p; Lung cancer antigen NY LU 1; Lung cancer antigen NY-LU-1; MGC10942; MGC17716; MGC17767; Muscle type aldolase; Muscle-type aldolase; RNALDOG5

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

2-364aa

Target Protein Sequence

PYQYPALTPEQKKELSDIAHRIVAPGKGILAADESTGSIAKRLQSIGTENTEENRRFYRQLLLTADDRVNPCIGGVILFHETLYQKADDGRPFPQVIKSKGGVVGIKVDKGVVPLAGTNGETTTQGLDGLSERCAQYKKDGADFAKWRCVLKIGEHTPSALAIMENANVLARYASICQQNGIVPIVEPEILPDGDHDLKRCQYVTEKVLAAVYKALSDHHIYLEGTLLKPNMVTPGHACTQKFSHEEIAMATVTALRRTVPPAVTGITFLSGGQSEEEASINLNAINKCPLLKPWALTFSYGRALQASALKAWGGKKENLKAAQEEYVKRALANSLACQGKYTPSGQAGAAASESLFVSNHAY
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

66.3kDa

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal GST-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Enhance your metabolic research with our Recombinant Human ALDOA protein, a crucial enzyme involved in glycolysis and gluconeogenesis. Fructose-bisphosphate aldolase A, encoded by the ALDOA gene, plays a vital role in energy production within cells by catalyzing the reversible cleavage of fructose-1,6-bisphosphate to dihydroxyacetone phosphate and glyceraldehyde-3-phosphate. This protein is also identified as a lung cancer antigen, NY-LU-1, making it relevant for cancer research.

Our Recombinant Human ALDOA protein is expressed in an E.coli system and encompasses the full length of the mature protein, covering the 2-364aa region. The N-terminal GST-tag allows for effective protein purification, ensuring a high-quality product for your experiments. With a purity greater than 90% as confirmed by SDS-PAGE, this full-length ALDOA protein delivers the reliability you need. Select either liquid or lyophilized powder form to suit your specific laboratory requirements and propel your research forward.

Target Background

Function

Plays a key role in glycolysis and gluconeogenesis. In addition, may also function as scaffolding protein.

Gene References into Functions

Results showed that ALDOA was upregulated in renal cell carcinoma (RCC) samples and cell lines and significantly associated with metastasis and survival. Overall, data revealed that ALDOA functions as a tumor promoter, plays a prominent role in proliferation, migration, and invasion of RCC cells with high expression, and may promote EMT and activate the Wnt/betacatenin signaling pathway. PMID: 29693182
The results indicate that ALDOA and PGK1 may indicate resistance to cisplatin in osteosarcoma PMID: 29199648
Knockdown of ALDOA in QBC939 and RBE cells attenuated the cell proliferation and induced a higher apoptosis rate. PMID: 30121648
Silencing aldolase A suppressed colon cancer cell proliferation and invasion and inhibited the epithelial-mesenchymal transition phenotype. Aldolase A protein expression in colon cancer was related to tumor location, tumor clinical stage and survival. PMID: 29453983
mir-122 and its targets G6PC3, ALDOA and CS play roles in the hypoxia responses that regulate glucose and energy metabolism and can serve as hypoxia biomarkers. PMID: 27793029
Aldolase A promotes lung cancer metastasis via PHD-mediated stabilization of HIF-1alpha and the subsequent activation of MMP9. PMID: 28610954
Findings show that ALDOA expression is up-regulated in colorectal cancer (CRC) and is a hypoxia-inducible prognostic factor that is closely related to CRC malignancy. PMID: 28000858
Overexpression of ALDOA is associated with colorectal cancer. PMID: 27468721
In vitro and in vivo results demonstrated that ALDOA was associated with proliferation and metastasis of pancreatic cancer cells. PMID: 26854714
Study shows that PI3K directly coordinates glycolysis with cytoskeletal dynamics in an AKT-independent manner. Growth factors or insulin stimulate the PI3K-dependent activation of Rac, leading to disruption of the actin cytoskeleton, release of filamentous actin-bound aldolase A, and an increase in aldolase activity. PMID: 26824656
Our results expand the clinical spectrum of aldolase A deficiency to isolated temperature-dependent rhabdomyolysis, and suggest that thermolability may be tissue specific. We also propose a treatment for this severe disease PMID: 25392908
Study provides evidence supporting a critical functional role of ALDOA in osteosarcoma progression, metastasis and perhaps chemoresistance. PMID: 25215901
ALDOC, Aldolase A (ALDOA) and Aldolase B (ALDOB) activate Wnt signaling. PMID: 24993527
ALDOA is highly expressed in lung squamous cell carcinoma (LSCC) and its expression level is correlated with LSCC metastasis, grades, differentiation status and poor prognosis. PMID: 24465716
The expression of ALDOA and/or SULT1A3 is significantly higher. PMID: 22949271
The results presented here point to ALDA as a factor involved in the regulation of cells proliferation. PMID: 23886627
The new prognostic biomarkers GRP78, Fructose-bisphosphate Aldolase A (ALDOA), Carbonic Anhydrase I (CA1) and Peptidyl-prolyl cis-trans isomerase A or Cyclophilin A (PPIA)) provided good survival prediction for TNM stage I-IV patients. PMID: 22996014
ALDOA is a target gene of the BACH1 transcription factor according to ChIP-seq analysis in HEK 293 cells. PMID: 21555518
Data that aldolase forms a complex with ARNO/Arf6 and the V-ATPase and that it may contribute to remodeling of the actin cytoskeleton. PMID: 21307348
Posttranslational nitration of aldolase A may be an important pathway that regulates mast cell phenotype and function. PMID: 20511553
ALDOA is involved in keratinocyte migration following the induction of lamellipodia formation, and ALDOA-related migration is enhanced by EGF PMID: 20362419
melanoma antigen expressed in G361, a representative melanoma cell line/ reacted with autoantibodies in patient sera PMID: 20181627
Gly346 is crucial for the correct conformation and function of aldolase A, because it governs the entry/release of the substrates into/from the enzyme cleft, and/or allows important C-terminal residues to approach the active site. PMID: 14766013
The existence of highly AMP-sensitive muscle-like FBPase, activity of which is regulated by metabolite-dependent interaction with aldolase enables the precise regulation of muscle energy expenditures. PMID: 18214967
Results identify VDAC2 and aldolase A as membrane proteins of K562 cells with increased expression under iron deprivation. PMID: 18278581
Aldolase A may play a role in the radio-response of human cells, probably in nuclei, in addition to its glycolytic role in the cytosol. PMID: 18328256
ZNF224 recruits the arginine methyltransferase PRMT5 on the transcriptional repressor complex of the aldolase A gene PMID: 19741270

Hide All

Involvement in disease

Glycogen storage disease 12 (GSD12)

Subcellular Location

Cytoplasm, myofibril, sarcomere, I band. Cytoplasm, myofibril, sarcomere, M line.

Protein Families

Class I fructose-bisphosphate aldolase family

Database Links

HGNC: 414

OMIM: 103850

KEGG: hsa:226

STRING: 9606.ENSP00000336927

UniGene: Hs.513490

Code	CSB-EP001583HU
Abbreviation	Recombinant Human ALDOA protein
MSDS	MSDS Datasheet
Size	$224
	Order now
Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-RP000154h could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) ALDOA. Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-RP000154h could indicate that this peptide derived from E.coli-expressed Homo sapiens (Human) ALDOA.
Have Questions?	Leave a Message or Start an on-line Chat

Recombinant Human Fructose-bisphosphate aldolase A (ALDOA)

Product Details

Citations

Related Products

Customer Reviews and Q&A

Target Background

×CloseMessage