ALDOA Recombinant Monoclonal Antibody

Datasheet

Code	CSB-RA571479A0HU
Size	US$210
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Image	Western Blot Positive WB detected in: Hela whole cell lysate, HepG2 whole cell lysate, NIH/3T3 whole cell lysate, A549 whole cell lysate, Mouse Liver whole cell lysate, Rat Liver whole cell lysate All lanes: Aldolase antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 40, 46 kDa Observed band size: 40 kDa IHC image of CSB-RA571479A0HU diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a Goat anti-rabbit IgG polymer labeled by HRP and visualized using 0.05% DAB. Immunofluorescence staining of Hela Cells with CSB-RA571479A0HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% TritonX-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4℃. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L).
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Product Details
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Target Background

Product Details

Uniprot No.

P04075

Target Names

ALDOA

Alternative Names

Fructose-bisphosphate aldolase A (EC 4.1.2.13) (Lung cancer antigen NY-LU-1) (Muscle-type aldolase), ALDOA, ALDA

Species Reactivity

Human, Mouse, Rat

Immunogen

A synthesized peptide derived from human Aldolase

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

7D8

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:200
IF	1:20-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

Aldolase A (ALDOA) is a glycolytic enzyme that catalyzes the reversible conversion of fructose-1,6-bisphosphate to glyceraldehyde-3-phosphate and dihydroxyacetone phosphate, positioning it at a critical juncture in cellular energy metabolism. Beyond its canonical role in glycolysis, ALDOA has emerged as a multifunctional protein implicated in cancer progression, where its overexpression correlates with enhanced tumor metabolism and proliferative capacity. Originally identified as the lung cancer antigen NY-LU-1, this target offers researchers a valuable entry point for investigating metabolic reprogramming in malignancy and related signaling pathways.

This recombinant monoclonal antibody, generated from clone 7D8 in rabbit host, provides the reproducibility essential for longitudinal studies and multi-site collaborations. Because the antibody sequence is defined and production occurs through recombinant expression, you can expect consistent performance across lots, eliminating the variability that often complicates experimental comparisons over time.

Validation studies demonstrate robust performance across multiple experimental platforms. Western blot analysis detects a clean band at the expected 40 kDa molecular weight across human cell lines including HeLa, HepG2, and A549, with confirmed cross-species reactivity in mouse and rat liver lysates as well as NIH/3T3 cells. Immunohistochemistry staining in paraffin-embedded human colon cancer tissue reveals clear target localization, while immunofluorescence in HeLa cells enables subcellular visualization of ALDOA distribution. This application flexibility allows you to move seamlessly between protein quantification, tissue-level expression analysis, and cellular localization studies within a single research program.

Whether your work focuses on tumor metabolism, glycolytic pathway regulation, or cancer biomarker validation, this antibody delivers the specificity and consistency needed to advance your investigations with confidence.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Plays a key role in glycolysis and gluconeogenesis. In addition, may also function as scaffolding protein.

Gene References into Functions

Results showed that ALDOA was upregulated in renal cell carcinoma (RCC) samples and cell lines and significantly associated with metastasis and survival. Overall, data revealed that ALDOA functions as a tumor promoter, plays a prominent role in proliferation, migration, and invasion of RCC cells with high expression, and may promote EMT and activate the Wnt/betacatenin signaling pathway. PMID: 29693182
The results indicate that ALDOA and PGK1 may indicate resistance to cisplatin in osteosarcoma PMID: 29199648
Knockdown of ALDOA in QBC939 and RBE cells attenuated the cell proliferation and induced a higher apoptosis rate. PMID: 30121648
Silencing aldolase A suppressed colon cancer cell proliferation and invasion and inhibited the epithelial-mesenchymal transition phenotype. Aldolase A protein expression in colon cancer was related to tumor location, tumor clinical stage and survival. PMID: 29453983
mir-122 and its targets G6PC3, ALDOA and CS play roles in the hypoxia responses that regulate glucose and energy metabolism and can serve as hypoxia biomarkers. PMID: 27793029
Aldolase A promotes lung cancer metastasis via PHD-mediated stabilization of HIF-1alpha and the subsequent activation of MMP9. PMID: 28610954
Findings show that ALDOA expression is up-regulated in colorectal cancer (CRC) and is a hypoxia-inducible prognostic factor that is closely related to CRC malignancy. PMID: 28000858
Overexpression of ALDOA is associated with colorectal cancer. PMID: 27468721
In vitro and in vivo results demonstrated that ALDOA was associated with proliferation and metastasis of pancreatic cancer cells. PMID: 26854714
Study shows that PI3K directly coordinates glycolysis with cytoskeletal dynamics in an AKT-independent manner. Growth factors or insulin stimulate the PI3K-dependent activation of Rac, leading to disruption of the actin cytoskeleton, release of filamentous actin-bound aldolase A, and an increase in aldolase activity. PMID: 26824656
Our results expand the clinical spectrum of aldolase A deficiency to isolated temperature-dependent rhabdomyolysis, and suggest that thermolability may be tissue specific. We also propose a treatment for this severe disease PMID: 25392908
Study provides evidence supporting a critical functional role of ALDOA in osteosarcoma progression, metastasis and perhaps chemoresistance. PMID: 25215901
ALDOC, Aldolase A (ALDOA) and Aldolase B (ALDOB) activate Wnt signaling. PMID: 24993527
ALDOA is highly expressed in lung squamous cell carcinoma (LSCC) and its expression level is correlated with LSCC metastasis, grades, differentiation status and poor prognosis. PMID: 24465716
The expression of ALDOA and/or SULT1A3 is significantly higher. PMID: 22949271
The results presented here point to ALDA as a factor involved in the regulation of cells proliferation. PMID: 23886627
The new prognostic biomarkers GRP78, Fructose-bisphosphate Aldolase A (ALDOA), Carbonic Anhydrase I (CA1) and Peptidyl-prolyl cis-trans isomerase A or Cyclophilin A (PPIA)) provided good survival prediction for TNM stage I-IV patients. PMID: 22996014
ALDOA is a target gene of the BACH1 transcription factor according to ChIP-seq analysis in HEK 293 cells. PMID: 21555518
Data that aldolase forms a complex with ARNO/Arf6 and the V-ATPase and that it may contribute to remodeling of the actin cytoskeleton. PMID: 21307348
Posttranslational nitration of aldolase A may be an important pathway that regulates mast cell phenotype and function. PMID: 20511553
ALDOA is involved in keratinocyte migration following the induction of lamellipodia formation, and ALDOA-related migration is enhanced by EGF PMID: 20362419
melanoma antigen expressed in G361, a representative melanoma cell line/ reacted with autoantibodies in patient sera PMID: 20181627
Gly346 is crucial for the correct conformation and function of aldolase A, because it governs the entry/release of the substrates into/from the enzyme cleft, and/or allows important C-terminal residues to approach the active site. PMID: 14766013
The existence of highly AMP-sensitive muscle-like FBPase, activity of which is regulated by metabolite-dependent interaction with aldolase enables the precise regulation of muscle energy expenditures. PMID: 18214967
Results identify VDAC2 and aldolase A as membrane proteins of K562 cells with increased expression under iron deprivation. PMID: 18278581
Aldolase A may play a role in the radio-response of human cells, probably in nuclei, in addition to its glycolytic role in the cytosol. PMID: 18328256
ZNF224 recruits the arginine methyltransferase PRMT5 on the transcriptional repressor complex of the aldolase A gene PMID: 19741270

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Involvement in disease

Glycogen storage disease 12 (GSD12)

Subcellular Location

Cytoplasm, myofibril, sarcomere, I band. Cytoplasm, myofibril, sarcomere, M line.

Protein Families

Class I fructose-bisphosphate aldolase family

Database Links

HGNC: 414

OMIM: 103850

KEGG: hsa:226

STRING: 9606.ENSP00000336927

UniGene: Hs.513490

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Related pathways

HIF-1 signaling pathway Target Therapy

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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