Recombinant Human Galactokinase (GALK1)

Code CSB-YP009198HU
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP009198HU
MSDS
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Source E.coli
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Code CSB-EP009198HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP009198HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP009198HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
GALK1
Uniprot No.
Alternative Names
EC 2.7.1.6; epididymis secretory protein Li 19; Galactokinase 1; Galactokinase; Galactose kinase; GALK 1; GALK; GALK1; GALK1_HUMAN; GK1; GLK; GLK1; HEL-S-19
Species
Homo sapiens (Human)
Expression Region
1-392
Target Protein Sequence
MAALRQPQVA ELLAEARRAF REEFGAEPEL AVSAPGRVNL IGEHTDYNQG LVLPMALELM TVLVGSPRKD GLVSLLTTSE GADEPQRLQF PLPTAQRSLE PGTPRWANYV KGVIQYYPAA PLPGFSAVVV SSVPLGGGLS SSASLEVATY TFLQQLCPDS GTIAARAQVC QQAEHSFAGM PCGIMDQFIS LMGQKGHALL IDCRSLETSL VPLSDPKLAV LITNSNVRHS LASSEYPVRR RQCEEVARAL GKESLREVQL EELEAARDLV SKEGFRRARH VVGEIRRTAQ AAAALRRGDY RAFGRLMVES HRSLRDDYEV SCPELDQLVE AALAVPGVYG SRMTGGGFGG CTVTLLEASA APHAMRHIQE HYGGTATFYL SQAADGAKVL CL
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Major enzyme for galactose metabolism.
Gene References into Functions
  1. Mutation in the GALK gene is associated with mental disorders in galactosemia. PMID: 28672748
  2. The GALK1 gene was included in this interval and direct sequencing. PMID: 24211322
  3. Data indicate taht the interactions between galactokinase (GALK) and its potential inhibitors by molecular dynamics simulations. PMID: 23517731
  4. The study highlighted the importance of GALK gene analysis in diagnosis of galactosemia in Indian population. PMID: 22632133
  5. A possible mechanism for the unfolding caused by the Pro(28)Thr point mutation of human galactokinase. PMID: 21264483
  6. Pathogenic mutations in GALK1 that are responsible for autosomal recessive congenital cataracts in consanguineous Pakistani families, are reported. PMID: 20405025
  7. 2 new GALK1 mutations near the ATP-binding site were found in a homozygotic Turkish immigrant: S142I and G148C. PMID: 11978883
  8. a functional analysis of disease-causing mutations in this enzyme PMID: 12694189
  9. In this northern Italian population age-related cataract does not appear to be associated with GALK1 alleles. PMID: 12942049
  10. structure and function of galactokinase, and role in type II galactosemia [review] PMID: 15526155
  11. active site geometry of this enzyme upon which to more fully understand the consequences of the those mutations known to give rise to Type II galactosemia. PMID: 15590630
  12. Mutations in GALK1 resulted in reduction of GALK activity and caused GALK deficiency. PMID: 17517531
  13. These results suggest that the elevated GALK1 activity resulted from enhanced gene expression, due to nucleotide variation within GALK1 promoter PMID: 19309526
  14. Structure of the human enzyme complexed with MgAMP.PNP and galactose PMID: 15590630
  15. The disease-causing point mutations in the human enzyme are mapped onto the structure of the protein from Pyrococcus furiosus and speculations made about the structural consequences. PMID: 15003454
  16. 2-deoxy-D-galactose is a substrate for this enzyme. D-glucose, D-fucose, L-arabinose and N-acetyl-D-galactosamine are not. Mutations H44A, H44I, E43G/H44I are insoluble, D46A is inactive, E43G has reduced activity and E43A has wild-type activity. PMID: 14596685
  17. Has ordered ternary complex mechanism with ATP being the first substrate to bind. PMID: 12694189

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Involvement in disease
Galactosemia II (GALCT2)
Protein Families
GHMP kinase family, GalK subfamily
Database Links

HGNC: 4118

OMIM: 230200

KEGG: hsa:2584

STRING: 9606.ENSP00000225614

UniGene: Hs.407966

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