Recombinant Human Gamma-aminobutyric acid type B receptor subunit 1(GABBR1) ,partial

Code CSB-YP871387HU
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Source Yeast
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Code CSB-EP871387HU
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Source E.coli
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Code CSB-EP871387HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP871387HU
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Source Baculovirus
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Code CSB-MP871387HU
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names GABBR1
Uniprot No. Q9UBS5
Alternative Names dJ271M21.1.1; dJ271M21.1.2; FLJ92613; GABA-B receptor 1; GABA-B-R1; GABA-BR1; GABAB R1; GABAB subunit 1c; GABABR1; GABBR1 3; GABBR1; GABR1_HUMAN; Gamma aminobutyric acid (GABA) B receptor 1; Gamma-aminobutyric acid type B receptor subunit 1; Gb1; GPRC3A; Seven transmembrane helix receptor
Species Homo sapiens (Human)
Protein Length Partial
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
Component of a heterodimeric G-protein coupled receptor for GABA, formed by GABBR1 and GABBR2. Within the heterodimeric GABA receptor, only GABBR1 seems to bind agonists, while GABBR2 mediates coupling to G proteins. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of down-stream effectors, such as adenylate cyclase. Signaling inhibits adenylate cyclase, stimulates phospholipase A2, activates potassium channels, inactivates voltage-dependent calcium-channels and modulates inositol phospholipid hydrolysis. Calcium is required for high affinity binding to GABA. Plays a critical role in the fine-tuning of inhibitory synaptic transmission. Pre-synaptic GABA receptor inhibits neurotransmitter release by down-regulating high-voltage activated calcium channels, whereas postsynaptic GABA receptor decreases neuronal excitability by activating a prominent inwardly rectifying potassium (Kir) conductance that underlies the late inhibitory postsynaptic potentials. Not only implicated in synaptic inhibition but also in hippocampal long-term potentiation, slow wave sleep, muscle relaxation and antinociception (Probable). Activated by (-)-baclofen, cgp27492 and blocked by phaclofen.; Isoform 1E may regulate the formation of functional GABBR1/GABBR2 heterodimers by competing for GABBR2 binding. This could explain the observation that certain small molecule ligands exhibit differential affinity for central versus peripheral sites.
Gene References into Functions
  1. GABA B receptor expression in myometrium PMID: 30343129
  2. Results show that pregnancy anxiety is associated with differential DNA methylation patterns in newborns and, reveal a potential role for GABBR1 methylation in association with stress in newborns. PMID: 29026448
  3. GABAB receptor modulation in Periodontal Ligament cells might become an important target in immunoinflammatory settings. PMID: 27812916
  4. There was no statistically significant association for the two SNPs of the GABBR1 gene (rs29230 and rs29267). However, a significant difference between AUD individuals and controls was observed at genotype level for rs2900512 of GABBR2 gene. PMID: 28118741
  5. The combined analysis confirmed evidence of genome-wide significant associations in the Han Chinese population for three loci, at 2p16.1 (rs1051061, in an exon of VRK2, P=1.14 x 10-12, odds ratio (OR)=1.17), 6p22.1 (rs115070292 in an intron of GABBR1, P=4.96 x 10-10, OR=0.77) and 10q24.32 (rs10883795 in an intron of AS3MT, P=7.94 x 10-10, OR=0.87; rs10883765 at an intron of ARL3, P=3.06 x 10-9, OR=0.87). PMID: 27922604
  6. Genotype and allele frequencies of rs29230 in GABR1 were significantly different between cases and controls, especially for male patients, for obstructive sleep apnea risk in Chinese Han population. PMID: 27509181
  7. presynaptic gamma-aminobutyric acid type B receptor deficits contribute to altered neuronal excitability in fragile X syndrome PMID: 28213518
  8. Results show that GABBR1 minor genotypes/alleles were protective against risk for alcoholism in 3 ethnically diverse cohorts. PMID: 26727527
  9. Cell surface ubiquitination precedes endocytosis, after which USP14 acts as an ubiquitin-binding protein that targets the ubiquitinated GABA B receptor to lysosomal degradation and promotes its deubiquitination. PMID: 26817839
  10. GABA(B)R stimulation promotes chemotaxis in RBL cells which is dependent on signaling via PI3-K/Akt, Src kinases and on rearrangement of both microtubules and actin cytoskeleton. PMID: 25725285
  11. GABBR1 receptors are expressed in aortic smooth muscle cells and regulate the [Ca(2+)]i via a Gi/o-coupled receptor pathway and a phospholipase C activation pathway. PMID: 24682435
  12. the first report of abnormal levels of GABA+ and Glx in mood-related brain regions of women with PMDD, indicating that dysregulation of the amino acid neurotransmitter system may be an important neurobiological mechanism in the pathogenesis of PMDD. PMID: 25465316
  13. Chronic alcohol altered exon/intron expression and splice junction levels. PMID: 24209778
  14. GABBR1 protein involved in phenylthiourea bitter taste detection. PMID: 24006265
  15. The endoplasmic reticulum retention signal of GBR1 is not part of the core coiled-coil structure, suggesting that it is sterically shielded by GBR2 upon heterodimer formation. PMID: 24778228
  16. Authors suggest GABBR1, GABA receptor B1, is implicated in schizophrenia based on a Human endogenous retrovirus long terminal repeat (HERV-W LTR) in the regulatory region of GABBR1. PMID: 23391219
  17. GABA may inhibit the growth of cholangiocarcinoma QBC939 cells through the GABAB receptor, and the anti-cancer effects may be partly mediated via the JAK/STAT3 pathway PMID: 23007731
  18. Activation of GABA(B) receptors significantly inhibits Akt/GSK-3 signaling in a beta-arrestin-dependent pathway. PMID: 23192081
  19. We have discovered main triplets of amino acid residues in the GABAB1 receptor and several other neural receptors which seem to come from immunoglobulin chains and appear also as homologies in receptor heteromers. PMID: 21932037
  20. Results show that GABA(B) receptors R1 and R2 must be activated for the modulation of N-type (Ca(v)2.2) calcium channels by analgesic alpha-conotoxins Vc1.1 and RgIA. PMID: 22613715
  21. This study does not support that GBR1 is involved in essential tremor. PMID: 22321358
  22. Long interval intracortical inhibition is significantly reduced and the cortical silent period is significantly shortened in patients with succinic semialdehyde dehydrogenase (SSADH) deficiency compared to heterozygous parents and control groups. PMID: 22722631
  23. The GBR2 ectodomain directly interacts with the GBR1 ectodomain to increase agonist affinity by selectively stabilizing the agonist-bound conformation of GBR1. PMID: 22660477
  24. The present meta-analysis suggests that GABBR1 G1465A polymorphism is associated with the risk of temporal lobe epilepsy. PMID: 21621395
  25. The results of this study provided evidence of Gabbr1 Deficit in schizophrenia and mood disorders. PMID: 21303731
  26. Data suggest that the GABAB1 intron 4 containing N-terminal truncation is necessary for the inhibitory action of the new splice variants. PMID: 21124972
  27. This chapter summarizes current understanding of the molecular function of the GABA(B) receptor and recent developments in the identification of allosteric modulators. PMID: 20655478
  28. This chapter focuses on the recently emerged mechanisms of GABA(B) receptor exocytosis, endocytosis, recycling, and degradation. PMID: 20655479
  29. The role that this phosphorylation plays in determining GABA(B)R effector coupling and their trafficking within the endocytic pathway, is discussed. PMID: 20655480
  30. The relationships between the GABA(B) receptor, its effectors and associated proteins that mediate GABA(B) receptor function within the brain, is described. PMID: 20655481
  31. Current knowledge on a new role of GABA(B)R as an ambience-dependent regulator of synaptic signaling, is presented. PMID: 20655482
  32. This chapter reviews data suggesting that neurogliaform cells produce electrophysiological effects onto other neurons in the cortical cell network via GABA(B)R-mediated volume transmission that is highly regulated by GAT1 activity. PMID: 20655483
  33. The overexpression of one particular gene encoding for G-protein-activated inward rectifying potassium type 2 (GIRK2) channel subunit and its coupling to GABA(B) receptors may contribute to a range of mental and functional disabilities in Down syndrome. PMID: 20655490
  34. Significant reductions in GABA(B) receptor subunit 1 density are demonstrated in cingulate cortex and fusiform gyrus from patients with autism compared to controls. PMID: 20557420
  35. GABAB1 subunits interact with DGCR6 in the endoplasmic reticulum prior to their recruitment into functional GABAB receptors. PMID: 20036641
  36. Data show that GABA is a potent chemoattractant of HUCB stem/progenitor cells specifically through GABA(B)R activation. PMID: 19327013
  37. variants of GABBR1 and GABBR2 are associated with nicotine dependence in European- and African-American populations PMID: 19763258
  38. Alterations in inhibitory synaptic transmission through GABA(B)R1 appears to affect differentially certain hippocampal circuits in a population of epileptic patients and could contribute to the pathophysiology of temporal lobe epilepsy. PMID: 12115687
  39. The intracellular loops of the GB2 subunit are crucial for G-protein coupling of the heteromeric gamma-aminobutyrate B receptor. PMID: 12130687
  40. evidence that the translated polymorphism of exon 7 may be functionally meaningful and impact cortical EEG oscillations PMID: 12555235
  41. The GABA(B[1]) polymorphism (G1465A) confers a highly increased susceptibility to temporal lobe epilepsy. Moreover, it seems to influence the severity of this common epileptic disorder PMID: 12601092
  42. Post hoc analyses showed an association between EEG phenotype and exon 7 genotype in normal subjects only. PMID: 12645817
  43. GABBR1 gene might not be a susceptibility gene for childhood absence epilepsy at least in the Chinese population PMID: 12770685
  44. GHB, administered in vivo, reduces MAP kinase phosphorylation via a direct activation of GABAB receptors by GHB, but was found ineffective on MAP kinase phosphorylation in brain slices PMID: 12923192
  45. Increased expression of GABA(B) receptor subtype 1 indicates augmented presynaptic inhibition of glutamate release as a possible protective mechanism in temporal lobe epilepsy. PMID: 14625043
  46. Altered GABA(B1a) receptor mRNA expression occurs in human temporal lobe epilepsy; possibly the observed changes may also serve to counteract ongoing hyperexcitability. PMID: 14643764
  47. GABAB receptor cell surface stability is modulated by phosphorylation and chronic agonist treatment PMID: 14707142
  48. GABA(B) receptor subunit GABA(B)R1 is found on the same neurons and follows the same distribution patterns in the basal ganglia as the GABABR2 receptor subunit. PMID: 14961561
  49. Association of the GABA(B)R1 with the GABA(A) receptor gamma2S subunit robustly promotes cell surface expression of GABA(B)R1 in the absence of GABA(B)R2, that is usually required for efficient trafficking of GABA(B)R1 to the cell surface. PMID: 14966130
  50. The GABA(B) receptor may be present as a heterodimer with subunits of GABA(B1) and GABA(B2) in the human colon. PMID: 14978362

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Subcellular Location Cell membrane; Multi-pass membrane protein. Cell junction, synapse, postsynaptic cell membrane; Multi-pass membrane protein. Cell projection, dendrite.; [Isoform 1E]: Secreted.
Protein Families G-protein coupled receptor 3 family, GABA-B receptor subfamily
Tissue Specificity Highly expressed in brain. Weakly expressed in heart, small intestine and uterus. Isoform 1A: Mainly expressed in granular cell and molecular layer. Isoform 1B: Mainly expressed in Purkinje cells. Isoform 1E: Predominantly expressed in peripheral tissues
Database Links

HGNC: 4070

OMIM: 603540

KEGG: hsa:2550

STRING: 9606.ENSP00000366233

UniGene: Hs.167017

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