Recombinant Human Grainyhead-like protein 1 homolog (GRHL1)

Code CSB-YP868368HU
MSDS
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Source Yeast
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Code CSB-EP868368HU
MSDS
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Source E.coli
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Code CSB-EP868368HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP868368HU
MSDS
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Source Baculovirus
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Code CSB-MP868368HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
GRHL1
Uniprot No.
Alternative Names
Grainyhead-like protein 1 homolog; grhl1; GRHL1_HUMAN; LBP32; Mammalian grainyhead; MGR; NH32; TFCP2L2; Transcription factor CP2-like 2; Transcription factor LBP-32
Species
Homo sapiens (Human)
Expression Region
1-618
Target Protein Sequence
MTQEYDNKRP VLVLQNEALY PQRRSYTSED EAWKSFLENP LTAATKAMMS INGDEDSAAA LGLLYDYYKV PRERRSSTAK PEVEHPEPDH SKRNSIPIVT EQPLISAGEN RVQVLKNVPF NIVLPHGNQL GIDKRGHLTA PDTTVTVSIA TMPTHSIKTE TQPHGFAVGI PPAVYHPEPT ERVVVFDRNL NTDQFSSGAQ APNAQRRTPD STFSETFKEG VQEVFFPSDL SLRMPGMNSE DYVFDSVSGN NFEYTLEASK SLRQKPGDST MTYLNKGQFY PITLKEVSSS EGIHHPISKV RSVIMVVFAE DKSREDQLRH WKYWHSRQHT AKQRCIDIAD YKESFNTISN IEEIAYNAIS FTWDINDEAK VFISVNCLST DFSSQKGVKG LPLNIQVDTY SYNNRSNKPV HRAYCQIKVF CDKGAERKIR DEERKQSKRK VSDVKVPLLP SHKRMDITVF KPFIDLDTQP VLFIPDVHFA NLQRGTHVLP IASEELEGEG SVLKRGPYGT EDDFAVPPST KLARIEEPKR VLLYVRKESE EVFDALMLKT PSLKGLMEAI SDKYDVPHDK IGKIFKKCKK GILVNMDDNI VKHYSNEDTF QLQIEEAGGS YKLTLTEI
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Transcription factor involved in epithelial development. Binds directly to the consensus DNA sequence 5'-AACCGGTT-3'. Important regulator of DSG1 in the context of hair anchorage and epidermal differentiation, participates in the maintenance of the skin barrier. There is no genetic interaction with GRHL3, no functional cooperativity due to diverse target gene selectivity during epithelia development.; Functions as transcription activator.; May function as a repressor in tissues where both isoform 1 and isoform 2 are expressed.
Gene References into Functions
  1. Non-melanoma skin cancer growth is accompanied by coordinated reduced expression of epidermal differentiation genes: GRHL1 and GRHL3, which may be regulated by miR-21-3p and -5p, respectively. Some potentially damaging single nucleotide polymorphisms in GRHL genes occur with altered frequencies in NMSC patients, and they may in particular impair the expression of GRHL3 gene or functioning of encoded protein PMID: 29301499
  2. All of these processes involve epithelial-mesencyhmal transition (EMT), MET or a sequence of both, suggesting that the GRHL factors((GRHL1, GRHL2 and GRHL3), could potentially affect tumor initiation and progression via EMT PMID: 28714958
  3. it was observed that ER stimulated gene expression by interacting with MEIS1 and FOXP3, and ER inhibited gene expression by interacting with THRB and GRHL1. PMID: 27035558
  4. Grhl1 deficiency affects inner ear development in zebrafish. PMID: 25896282
  5. GRHL1, GRHL2, and GRHL3 have roles in cellular proliferation, differentiation, adhesion, and polarity and may promote cancer or be tumor suppressors [review] PMID: 26069269
  6. Downregulation of miR122 by grainyhead-like 2 restricts the hepatocytic differentiation potential of adult liver progenitor cells. PMID: 25406394
  7. A significant role for HDAC3 in the MYCN-mediated repression of GRHL1. PMID: 24419085
  8. Our findings reveal important pathophysiological differences between human pulmonary fibrosis and specific mouse models of fibrosis and support a crucial role of GRHL2 in epithelial activation in lung fibrosis and perhaps also in epithelial plasticity. PMID: 24375798
  9. LBP-1b and LBP-9 both stimulate LBP-32/MGR promoter activity. PMID: 18004979
  10. Includes a comparison to TFCP2L2, the GRHL1 protein. PMID: 12393799

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Subcellular Location
Nucleus.
Protein Families
Grh/CP2 family, Grainyhead subfamily
Tissue Specificity
Isoform 1 is highly expressed in brain, pancreas, tonsil, placenta and kidney. Isoform 2 is highly expressed in brain and liver. Expressed at very low levels in non-steroidogenic cells.
Database Links

HGNC: 17923

OMIM: 609786

KEGG: hsa:29841

STRING: 9606.ENSP00000324693

UniGene: Hs.418493

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