GRHL1 Antibody

Datasheet

Code	CSB-PA868368LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, HepG2 whole cell lysate, A549 whole cell lysate All lanes: GRHL1 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 71, 50, 58 kDa Observed band size: 71 kDa IHC image of CSB-PA868368LA01HU diluted at 1:300 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-PA868368LA01HU diluted at 1:300 and staining in paraffin-embedded human brain tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) GRHL1 Polyclonal antibody

Uniprot No.

Q9NZI5

Target Names

GRHL1

Alternative Names

Grainyhead-like protein 1 homolog antibody; grhl1 antibody; GRHL1_HUMAN antibody; LBP32 antibody; Mammalian grainyhead antibody; MGR antibody; NH32 antibody; TFCP2L2 antibody; Transcription factor CP2-like 2 antibody; Transcription factor LBP-32 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Grainyhead-like protein 1 homologprotein (1-95AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The GRHL1 Antibody (Product code: CSB-PA868368LA01HU) is Non-conjugated. For GRHL1 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA868368LB01HU	GRHL1 Antibody, HRP conjugated	ELISA
FITC	CSB-PA868368LC01HU	GRHL1 Antibody, FITC conjugated
Biotin	CSB-PA868368LD01HU	GRHL1 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:200-1:500

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

Transcription factor involved in epithelial development. Binds directly to the consensus DNA sequence 5'-AACCGGTT-3'. Important regulator of DSG1 in the context of hair anchorage and epidermal differentiation, participates in the maintenance of the skin barrier. There is no genetic interaction with GRHL3, no functional cooperativity due to diverse target gene selectivity during epithelia development.; Functions as transcription activator.; May function as a repressor in tissues where both isoform 1 and isoform 2 are expressed.

Gene References into Functions

Non-melanoma skin cancer growth is accompanied by coordinated reduced expression of epidermal differentiation genes: GRHL1 and GRHL3, which may be regulated by miR-21-3p and -5p, respectively. Some potentially damaging single nucleotide polymorphisms in GRHL genes occur with altered frequencies in NMSC patients, and they may in particular impair the expression of GRHL3 gene or functioning of encoded protein PMID: 29301499
All of these processes involve epithelial-mesencyhmal transition (EMT), MET or a sequence of both, suggesting that the GRHL factors((GRHL1, GRHL2 and GRHL3), could potentially affect tumor initiation and progression via EMT PMID: 28714958
it was observed that ER stimulated gene expression by interacting with MEIS1 and FOXP3, and ER inhibited gene expression by interacting with THRB and GRHL1. PMID: 27035558
Grhl1 deficiency affects inner ear development in zebrafish. PMID: 25896282
GRHL1, GRHL2, and GRHL3 have roles in cellular proliferation, differentiation, adhesion, and polarity and may promote cancer or be tumor suppressors [review] PMID: 26069269
Downregulation of miR122 by grainyhead-like 2 restricts the hepatocytic differentiation potential of adult liver progenitor cells. PMID: 25406394
A significant role for HDAC3 in the MYCN-mediated repression of GRHL1. PMID: 24419085
Our findings reveal important pathophysiological differences between human pulmonary fibrosis and specific mouse models of fibrosis and support a crucial role of GRHL2 in epithelial activation in lung fibrosis and perhaps also in epithelial plasticity. PMID: 24375798
LBP-1b and LBP-9 both stimulate LBP-32/MGR promoter activity. PMID: 18004979
Includes a comparison to TFCP2L2, the GRHL1 protein. PMID: 12393799

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Subcellular Location

Nucleus.

Protein Families

Grh/CP2 family, Grainyhead subfamily

Tissue Specificity

Isoform 1 is highly expressed in brain, pancreas, tonsil, placenta and kidney. Isoform 2 is highly expressed in brain and liver. Expressed at very low levels in non-steroidogenic cells.

Database Links

HGNC: 17923

OMIM: 609786

KEGG: hsa:29841

STRING: 9606.ENSP00000324693

UniGene: Hs.418493

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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