Recombinant Human GrpE protein homolog 2, mitochondrial (GRPEL2)

Code CSB-YP819443HU
MSDS
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Source Yeast
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Code CSB-EP819443HU
MSDS
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Source E.coli
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Code CSB-EP819443HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP819443HU
MSDS
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Source Baculovirus
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Code CSB-MP819443HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
GRPEL2
Uniprot No.
Alternative Names
GrpE like 2; mitochondrial; GrpE protein homolog 2; GRPE2_HUMAN; GRPEL 2; Grpel2; mitochondrial; Mt GrpE#2; Mt-GrpE#2
Species
Homo sapiens (Human)
Expression Region
33-225
Target Protein Sequence
STATQRTA GEDCRSEDPP DELGPPLAER ALRVKAVKLE KEVQDLTVRY QRAIADCENI RRRTQRCVED AKIFGIQSFC KDLVEVADIL EKTTECISEE SEPEDQKLTL EKVFRGLLLL EAKLKSVFAK HGLEKLTPIG DKYDPHEHEL ICHVPAGVGV QPGTVALVRQ DGYKLHGRTI RLARVEVAVE SQRRL
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Essential component of the PAM complex, a complex required for the translocation of transit peptide-containing proteins from the inner membrane into the mitochondrial matrix in an ATP-dependent manner. Seems to control the nucleotide-dependent binding of mitochondrial HSP70 to substrate proteins. Stimulates ATPase activity of mt-HSP70. May also serve to modulate the interconversion of oligomeric (inactive) and monomeric (active) forms of mt-HSP70.
Gene References into Functions
  1. The dimerization of GRPEL2 may activate the folding machinery responsible for protein import into mitochondrial matrix or enhance the chaperone activity of mitochondrial HSP70, thus protecting mitochondrial proteostasis in oxidative stress. PMID: 30098457
Subcellular Location
Mitochondrion matrix.
Protein Families
GrpE family
Database Links

HGNC: 21060

KEGG: hsa:134266

STRING: 9606.ENSP00000329558

UniGene: Hs.511816

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