Recombinant Human Heme-binding protein 1 (HEBP1)

Code CSB-YP868305HU
MSDS
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Source Yeast
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Code CSB-EP868305HU
MSDS
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Source E.coli
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Code CSB-EP868305HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP868305HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP868305HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
HEBP1
Uniprot No.
Alternative Names
HBP; HEBP; Hebp1; HEBP1_HUMAN; Heme binding protein 1; Heme-binding protein 1; p22HBP
Species
Homo sapiens (Human)
Expression Region
1-189
Target Protein Sequence
MLGMIKNSLF GSVETWPWQV LSKGDKEEVA YEERACEGGK FATVEVTDKP VDEALREAMP KVAKYAGGTN DKGIGMGMTV PISFAVFPNE DGSLQKKLKV WFRIPNQFQS DPPAPSDKSV KIEEREGITV YSMQFGGYAK EADYVAQATR LRAALEGTAT YRGDIYFCTG YDPPMKPYGR RNEIWLLKT
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
May bind free porphyrinogens that may be present in the cell and thus facilitate removal of these potentially toxic compound. Binds with a high affinity to one molecule of heme or porphyrins. It binds metalloporphyrins, free porphyrins and N-methylprotoporphyrin with similar affinities.
Gene References into Functions
  1. The functional activity of purified full-length HEBP1 has been evaluated on human formyl peptide receptor (FPR)3-expressing CHO-K1 cells PMID: 21709160
  2. Results present a systematic molecular modeling study of the binding properties of murine and human p22HBP protein (heme-binding protein) with four tetrapyrrole ring systems belonging to the heme biosynthetic pathway. PMID: 20800521
  3. Characterization as a tetrapyrrole-binding protein PMID: 12413491
  4. F2L is a chemoattractant peptide promoting calcium mobilization and chemotaxis in dendritic cells and monocytes. [F2L] PMID: 15623572
  5. mouse neutrophils, which like macrophages and dendritic cells express Fpr2, responded to human and mouse F2L in both calcium flux and chemotaxis assays PMID: 17237393

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Subcellular Location
Cytoplasm.
Protein Families
HEBP family
Database Links

HGNC: 17176

OMIM: 605826

KEGG: hsa:50865

STRING: 9606.ENSP00000014930

UniGene: Hs.642618

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