Recombinant Human Histone-lysine N-methyltransferase SETD1B (SETD1B), partial

Code CSB-YP892167HU
MSDS
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Source Yeast
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Code CSB-EP892167HU
MSDS
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Source E.coli
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Code CSB-EP892167HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP892167HU
MSDS
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Source Baculovirus
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Code CSB-MP892167HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
SETD1B
Uniprot No.
Alternative Names
FLJ20803; Histone-lysine N-methyltransferase SETD1B; hSET1B; KIAA1076; KMT2G; Lysine N-methyltransferase 2G; SET domain containing 1B; SET domain-containing protein 1B; SET1B; SET1B_HUMAN; SETD1B
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Histone methyltransferase that catalyzes methyl group transfer from S-adenosyl-L-methionine to the epsilon-amino group of 'Lys-4' of histone H3 (H3K4) via a non-processive mechanism. Part of chromatin remodeling machinery, forms H3K4me1, H3K4me2 and H3K4me3 methylation marks at active chromatin sites where transcription and DNA repair take place. Plays an essential role in regulating the transcriptional programming of multipotent hematopoietic progenitor cells and lymphoid lineage specification during hematopoiesis.
Gene References into Functions
  1. KMT2G levels were higher in metastatic clear cell renal cell carcinomas and may be a good marker for discriminating metastatic tumors from non-metastatic tumors. PMID: 29944950
  2. report the existence of a cytoplasmic form of SET1B/COMPASS, which is essential for maintaining breast tumor cell viability and promotes cell growth. PMID: 29138278
  3. we propose histone demethylase KDM2B and histone methyltransferase SETD1B as the two most plausible candidate genes involved in intellectual disability, autism, epilepsy, and craniofacial anomalies PMID: 27106595
  4. We analyzed SETD1B expression in gastric and colorectal cancer. Loss of SETD1B was identified in 15% to 55% of the GC and CRC with respect to the microsatellite instability status. Loss of expression was more common in those with SETD1B mutations. PMID: 24925220
  5. both Rbm15 and the leukemogenic Rbm15-Mkl1 fusion protein interact with the Setd1b histone H3-Lys4 methyltransferase PMID: 22927943

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Subcellular Location
Nucleus speckle. Chromosome. Note=Localizes to a largely non-overlapping set of euchromatic nuclear speckles with SETD1A, suggesting that SETD1A and SET1B each bind to a unique set of target genes.
Protein Families
Class V-like SAM-binding methyltransferase superfamily
Database Links

HGNC: 29187

OMIM: 611055

STRING: 9606.ENSP00000267197

UniGene: Hs.507122

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