Recombinant Human Homeobox protein SIX6 (SIX6)

Code CSB-YP021353HU
MSDS
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Source Yeast
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Code CSB-EP021353HU
MSDS
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Source E.coli
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Code CSB-EP021353HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP021353HU
MSDS
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Source Baculovirus
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Code CSB-MP021353HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
SIX6
Uniprot No.
Alternative Names
Homeobox protein SIX6; Homeodomain protein OPTX2; MCOPCT 2; MCOPCT2; Optic homeobox 2; OPTX 2; OPTX2; Sine oculis homeobox homolog 6; SIX 6; Six 9; SIX homeobox 6; Six6; SIX6_HUMAN; Six9
Species
Homo sapiens (Human)
Expression Region
1-246
Target Protein Sequence
MFQLPILNFS PQQVAGVCET LEESGDVERL GRFLWSLPVA PAACEALNKN ESVLRARAIV AFHGGNYREL YHILENHKFT KESHAKLQAL WLEAHYQEAE KLRGRPLGPV DKYRVRKKFP LPRTIWDGEQ KTHCFKERTR HLLREWYLQD PYPNPSKKRE LAQATGLTPT QVGNWFKNRR QRDRAAAAKN RLQQQVLSQG SGRALRAEGD GTPEVLGVAT SPAASLSSKA ATSAISITSS DSECDI
Protein Length
Full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
May be involved in eye development.
Gene References into Functions
  1. The results suggest that SIX6 influences different stages of RGC differentiation and their survival; therefore, alteration in SIX6 function due to the risk allele may lead to cellular and molecular abnormalities. PMID: 28792678
  2. We replicated the association of SNP rs10483727 in the SIX1/SIX6 locus with POAG in a Saudi cohort, suggesting its role in increasing susceptibility to Primary Open Angle Glaucoma . PMID: 29190129
  3. Among 8 SNPs in 3 loci that showed at least nominal association (P < 5.00E-02) in the primary cohort, a representative SNP for each loci (rs2157719 for CDKN2B-AS1, rs33912345 for SIX6, and rs9913911 for GAS7) were selected PMID: 29261660
  4. elevated expressions of SIX2, SIX4, and SIX6 predicted poor overall survival (OS) in NSCLC and poor relapse-free survival (RFS) in lung adenocarcinoma PMID: 27821176
  5. study replicated the association of POAG with two SNPs at the SIX1-SIX6 locus and demonstrated that SNPs, rs10483727 and rs33912345, are significantly associated with POAG, especially with NTG in patients aged above 40 years PMID: 27260188
  6. Six1 regulates p53 via a dual mechanism involving upregulation of microRNA-27a and downregulation of RPL26. PMID: 26687066
  7. Single nucleotide polymorphism in SIX6 gene is associated with primary open angle glaucoma. PMID: 25798827
  8. SIX6 and/or intraocular pressure promotes primary open-angle glaucoma by directly increasing p16INK4a expression. PMID: 26365380
  9. we report a homozygous missense mutation of SIX6 associated with a unique eye phenotype characterized by optic disc anomalies, macular atrophy, and coloboma of the iris and chorioretina. PMID: 24702266
  10. Nonglaucomatous individuals with the SIX6 missense variant have reduced RNFL thickness in regions known to be particularly affected in those with glaucoma. PMID: 25537207
  11. Our results, in combination with previous SIX6 work, lead us to hypothesize that SIX6 risk variants disrupt the development of the neural retina, leading to a reduced number of retinal ganglion cells. PMID: 24875647
  12. Our findings in humans and zebrafish suggest a functional involvement of six6b in early eye development, and open new insights into the genetic architecture of POAG. PMID: 24150847
  13. No evidence was found that SIX6 mutations underlie human congenital structural eye malformations. PMID: 15505031
  14. Six6 and Six2 differ in their DNA-binding mechanisms and in the consequences of their interaction with protein tyrosine phosphatase Eyes Absent in Six2/6-DNA-binding ability. PMID: 18293925
  15. BOR and OAVS features are associated with duplication of SIX1, SIX6 and OTX2 resulting from a complex chromosomal rearrangement. PMID: 18666230

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Involvement in disease
Optic disk anomalies with retinal and/or macular dystrophy (ODRMD)
Subcellular Location
Nucleus.
Protein Families
SIX/Sine oculis homeobox family
Tissue Specificity
Expressed in the developing and adult retina. Also expressed in the hypothalamic and the pituitary regions.
Database Links

HGNC: 10892

OMIM: 212550

KEGG: hsa:4990

STRING: 9606.ENSP00000328596

UniGene: Hs.194756

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