Product Details

Purity

Greater than 90% as determined by SDS-PAGE.
Greater than 95% as determined by SEC-HPLC.

Target Names

PTK7

Uniprot No.

Q13308

Research Area

Cell Adhesion

Alternative Names

8430404F20Rik; CCK 4; CCK-4; Colon carcinoma kinase 4; Human colon carcinoma kinase 4 (CCK4) mRNA complete cds; Inactive tyrosine-protein kinase 7; mPTK7/CCK4; OTTHUMP00000217016; Protein tyrosine kinase PTK 7; Protein-tyrosine kinase 7; Pseudo tyrosine kinase receptor 7; PTK 7; PTK7; PTK7 protein tyrosine kinase 7; PTK7 protein tyrosine kinase; PTK7_HUMAN; Serum response factor; Srf; Tyrosine-protein kinase-like 7

Species

Homo sapiens (Human)

Source

Yeast

Expression Region

31-704aa

Target Protein Sequence

AIVFIKQPSSQDALQGRRALLRCEVEAPGPVHVYWLLDGAPVQDTERRFAQGSSLSFAAVDRLQDSGTFQCVARDDVTGEEARSANASFNIKWIEAGPVVLKHPASEAEIQPQTQVTLRCHIDGHPRPTYQWFRDGTPLSDGQSNHTVSSKERNLTLRPAGPEHSGLYSCCAHSAFGQACSSQNFTLSIADESFARVVLAPQDVVVARYEEAMFHCQFSAQPPPSLQWLFEDETPITNRSRPPHLRRATVFANGSLLLTQVRPRNAGIYRCIGQGQRGPPIILEATLHLAEIEDMPLFEPRVFTAGSEERVTCLPPKGLPEPSVWWEHAGVRLPTHGRVYQKGHELVLANIAESDAGVYTCHAANLAGQRRQDVNITVATVPSWLKKPQDSQLEEGKPGYLDCLTQATPKPTVVWYRNQMLISEDSRFEVFKNGTLRINSVEVYDGTWYRCMSSTPAGSIEAQARVQVLEKLKFTPPPQPQQCMEFDKEATVPCSATGREKPTIKWERADGSSLPEWVTDNAGTLHFARVTRDDAGNYTCIASNGPQGQIRAHVQLTVAVFITFKVEPERTTVYQGHTALLQCEAQGDPKPLIQWKGKDRILDPTKLGPRMHIFQNGSLVIHDVAPEDSGRYTCIAGNSCNIKHTEAPLYVVDKPVPEESEGPGSPPPYKMIQT
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

76.6kDa

Protein Length

Extracellular Domain

Tag Info

N-terminal 6xHis-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The synthesis of this Recombinant Human PTK7 protein depends on the utilization of recombinant DNA technology to a large degree. DNA sequences that encoded the PTK7 protein could be inserted into a vector and introduced into an expression host, Yeast, where it could be easily expressed in and purified from. The expression of this PTK7 protein was at 31-704aa. N-terminal 6xHis tag was fused with this protein. The purity is 90%+ determined by SDS-PAGE.

PTK7 (also known as CCK4) is gene encoding a protein named inactive tyrosine-protein kinase 7(PTK7) in human. The protein encoded by this gene is also known as colon carcinoma kinase 4 (CCK4), protein-tyrosine kinase 7, pseudo tyrosine kinase receptor 7 and tyrosine-protein kinase-like 7. The protein encoded by this gene is a positive regulator of canonical Wnt signaling pathway with its coreceptor activity. Moreover, this protein is also involved other biological processes, such as actin cytoskeleton reorganization, cell adhesion, cell migration and signal transduction.

Target Background

Function

Inactive tyrosine kinase involved in Wnt signaling pathway. Component of both the non-canonical (also known as the Wnt/planar cell polarity signaling) and the canonical Wnt signaling pathway. Functions in cell adhesion, cell migration, cell polarity, proliferation, actin cytoskeleton reorganization and apoptosis. Has a role in embryogenesis, epithelial tissue organization and angiogenesis.

Gene References into Functions

miR2055p is involved in the proliferation, migration and invasion of colorectal cancer cells through inhibiting PTK7. PMID: 29488611
Higher expression of PTK7 significantly indicates worse prognosis in human malignancies PMID: 28924970
Inhibition of PTK7 by siRNA treatment significantly decreases the viability of atypical teratoid rhabdoid tumors (ATRT)patient-derived tumor cell lines.These studies provide the groundwork for future preclinical in vivo studies aiming to investigate the efficacy of PTK7 inhibition on ATRT tumor growth PMID: 28442586
PTK7 localization and protein stability is affected by canonical Wnt ligands. PMID: 28420671
These findings demonstrate that PTK7 upregulates MMP9 through activation of AP-1 and NF-kappaB and, thus increases invasive properties of ESCC cells. PMID: 27689325
PTK7 overexpression is associated with esophageal squamous cell carcinoma. PMID: 28545451
Our data indicate that PTK7 protein expression is associated with the prognosis of oral tongue squamous cell carcinoma PMID: 28547980
PTK7 expression was correlated with tumor differentiation (P=0.027), lymph node metastasis (P=0.005), distant metastasis (P=0.001) and TNM stage (P=0.028) of colorectal cancer patients PMID: 27499181
High PTK7 expression is associated with radioresistance in esophageal squamous cell carcinoma. PMID: 27557627
PTK7 enriches in self-renewing, multipotent stem cells of the human colon. PMID: 26549850
findings implicate PTK7 as a risk factor for NTDs and provide additional evidence for a pathogenic role of PCP signaling in these malformations PMID: 26368655
overexpression in PTK7-negative cancer cells led to increased metastatic events. PTK7 expression thus represents a potential prognostic biomarker and a novel therapeutic target in CRC. PMID: 25962058
data demonstrates that PTK7 regulates the activity of KDR biphasically by inducing oligomerization of KDR molecules at lower concentrations and by surrounding KDR molecules at higher concentrations. PMID: 25986862
PTK7 regulates Id1 expression in CD44-high glioma cell lines. Targeting PTK7 could be an effective strategy for treating glioma with high CD44 expression. PMID: 25204555
The results suggest that N-acylethanolamine acid amidase and protein tyrosine kinase 7 may be used as potential tissue biomarkers to avoid overtreatment of non-aggressive prostate cancer PMID: 24741114
Expression level of PTK7 was significantly decreased from benign to malignant ovarian epithelial tumors. survival analysis showed that patients with negative expression of PTK7 protein had poorer outcome than those with positive expression. PMID: 25550828
PTK7 acts as a cancer-related gene and may be a potent prognostic marker for Hepatocellular carcinoma(HCC). PMID: 25796105
results provide convincing evidence that both PTK7 expression and proteolysis, rather than the level of the cellular full-length PTK7 alone, contribute to efficient directional cell motility and metastasis in cancer PMID: 25006253
PTK7 expression plays an important role in the invasiveness of intrahepatic cholangiocarcinoma (ICC) cells and leads to a poor prognosis in ICC patients. PMID: 24587299
PTK 7 is a transforming gene and prognostic marker for breast cancer and nodal metastasis involvement. PMID: 24409301
This work defines PTK7 as a highly and specifically expressed gene in adenocarcinoma and a potential therapeutic target in this subset of NSCLC. PMID: 24654231
Increased expression of PTK7 was inversely correlated with overall survival. PMID: 23663482
in patients treated by chemotherapy, patients with PTK7-negative tumors seemed to have better DFS than those with PTK7-positive tumors, particularly for patients treated with only anthracycline-therapy drugs PMID: 24023307
PTK7 is a novel marker of epithelial to mesenchymal transitions PMID: 23209741
cleavage of the PTK7 ectodomain by an ADAM proteinase was coupled with the membrane type-1 matrix metalloproteinase (MT1-MMP) cleavage of the PKP(621) downward arrowLI site in the seventh Ig-like domain of PTK7. PMID: 23095747
PTK7 has utility as a biomarker for detecting minimal residual disease of T cell acute lymphoblastic leukemia in the bone marrow PMID: 22898210
a novel role for PTK7 in the tumorigenesis via generation of PTK7-CTF2 by sequential cleavage of ADAM17 and gamma-secretase. PMID: 22665490
aberrations in the membrane type 1 matrix metalloproteinase/PTK7 axis are detrimental to cell movements that shape the body plan PMID: 21518755
Observations may indicate a role for PTK7 in cell proliferation and cell apoptosis and may provide a potential therapeutic pathway for the treatment of a variety of cancers. PMID: 21103379
Protein Tyrosine Kinase 7 is required for beta-catenin-dependent transcriptional events induced by canonical Wnt ligands PMID: 21132015
Results indicate that PTK7 is a major target of MT1-MMP. PMID: 20837484
PTK7 is a planar cell polarity component expressed in the myeloid progenitor compartment that conveys promigratory and antiapoptotic signals into the cell and represents an independent prognosis factor in patients treated with induction chemotherapy. PMID: 20558616
cloning, sequencing and expression of gene and its splice variants PMID: 12427550
Lack of a thermodynamically meaningful self-association propensity for the CCK4 transmembrane domains demonstrates that the conserved GxxxG motif is not sufficient to drive CCK4 transmembrane helix-helix interactions. PMID: 15683231
mechanism of PTK7 upregulation in colorectal cancer PMID: 17671748
PTK7 plays an important role not only in tube formation, migration, and invasion of endothelial cells but also in angiogenesis. PMID: 18471990

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Subcellular Location

Membrane; Single-pass type I membrane protein. Cell junction. Note=Colocalizes with MMP14 at cell junctions. Also localizes at the leading edge of migrating cells.

Protein Families

Protein kinase superfamily, Tyr protein kinase family, Insulin receptor subfamily

Tissue Specificity

Highly expressed in lung, liver, pancreas, kidney, placenta and melanocytes. Weakly expressed in thyroid gland, ovary, brain, heart and skeletal muscle. Also expressed in erythroleukemia cells. But not expressed in colon.

Database Links

HGNC: 9618

OMIM: 601890

KEGG: hsa:5754

STRING: 9606.ENSP00000230419

UniGene: Hs.90572

Code	CSB-YP622651HU
Abbreviation	Recombinant Human PTK7 protein, partial
MSDS	MSDS Datasheet
Size	$250
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Based on the SEQUEST from database of Yeast host and target protein, the LC-MS/MS Analysis result of CSB-YP622651HU could indicate that this peptide derived from Yeast-expressed Homo sapiens (Human) PTK7. Based on the SEQUEST from database of Yeast host and target protein, the LC-MS/MS Analysis result of CSB-YP622651HU could indicate that this peptide derived from Yeast-expressed Homo sapiens (Human) PTK7. The purity of PTK7 was greater than 95% as determined by SEC-HPLC
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Recombinant Human Inactive tyrosine-protein kinase 7 (PTK7), partial

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