Recombinant Human Insulin receptor substrate 4(IRS4) ,partial

Code CSB-YP011832HU
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Source Yeast
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Code CSB-EP011832HU
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Source E.coli
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Code CSB-EP011832HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP011832HU
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Source Baculovirus
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Code CSB-MP011832HU
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names IRS4
Uniprot No. O14654
Alternative Names IRS4; Insulin receptor substrate 4; IRS-4; 160 kDa phosphotyrosine protein; py160; Phosphoprotein of 160 kDa; pp160
Species Homo sapiens (Human)
Protein Length Partial
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
Acts as an interface between multiple growth factor receptors possessing tyrosine kinase activity, such as insulin receptor, IGF1R and FGFR1, and a complex network of intracellular signaling molecules containing SH2 domains. Involved in the IGF1R mitogenic signaling pathway. Promotes the AKT1 signaling pathway and BAD phosphorylation during insulin stimulation without activation of RPS6KB1 or the inhibition of apoptosis. Interaction with GRB2 enhances insulin-stimulated mitogen-activated protein kinase activity. May be involved in nonreceptor tyrosine kinase signaling in myoblasts. Plays a pivotal role in the proliferation/differentiation of hepatoblastoma cell through EPHB2 activation upon IGF1 stimulation. May play a role in the signal transduction in response to insulin and to a lesser extent in response to IL4 and GH on mitogenesis. Plays a role in growth, reproduction and glucose homeostasis. May act as negative regulators of the IGF1 signaling pathway by suppressing the function of IRS1 and IRS2.
Gene References into Functions
  1. increase of IRS-4 expression may be involved to some extent in colorectal cancer. PMID: 29185229
  2. IRS4 overexpression is associated with cancer. PMID: 27869826
  3. these results demonstrated that miR-493 acts as a tumor suppressor and inhibits cell proliferation and cell cycle in human melanoma by directly targeting IRS4. PMID: 28475006
  4. The association of IRS4 with SSH1 contributes to localized activation of cofilin in membrane protrusions. PMID: 25100728
  5. Overexpression of IRS4 in U2OS cells activates PI3K signalling. PMID: 24039912
  6. We genotyped single-nucleotide polymorphisms of IGF1, IGF2, IGF1R, IGF2R, IGFBP1, IGFBP3, IGFBP5, IRS1, IRS2, and IRS in pancreatic cancer patients PMID: 21852217
  7. This study clearly demonstrates associations between body mass index and IRS-4 variants in schizophrenia patients, but not in healthy controls, pointing to a possible involvement of IRS-4 in the control of body weight in schizophrenia. PMID: 22167131
  8. Study for the first time identified IRS4 mutations in T-ALL. PMID: 21517825
  9. IRS-4 gene is not of importance for aetiology of the vast majority of schizophrenia cases, but a single patient with schizophrenia and a mutation in IRS-4 points to that the insulin signalling system is of interest in the search for schizophrenia genes PMID: 21407155
  10. The t(X;6) in subungual exostosis results in transcriptional deregulation of the gene for insulin receptor substrate 4. PMID: 20340132
  11. Insulin receptor substrate 4 associates with the protein IRAS PMID: 11912194
  12. IRS-4 does not function as a substrate of the insulin and the IGF-I receptor in primary muscle cells but may be involved in nonreceptor tyrosine kinase signaling. PMID: 12639902
  13. data demonstrate cell-specific alterations in IRS protein concentrations in theca cells from polycystic ovaries consistent with exaggerated amplification of the insulin signal & which may play a role in ovarian hyperandrogenism & thecal hyperplasia PMID: 15155816
  14. insulin receptor substrate-4 and ShcA have roles in signaling by the fibroblast growth factor receptor PMID: 15316024
  15. IRS-4 is subject to regulation by TNF-alpha, and PP4 mediates TNF-alpha-induced degradation of IRS-4 PMID: 15331607
  16. endogenous Brk and IRS-4 interact in A431 human epidermoid carcinoma cells PMID: 15870689
  17. IRS-4 plays an important role in HepG2 proliferation/differentiation and exerts its actions through ERK and p70S6K activation in a ras/raf/MEK1/2- and PI3Kinase/Akt-independent manner and in a PKC-dependent way PMID: 17408801
  18. Transgenic rat gene WDR6 interacts with insulin receptor substrate 4 (IRS-4) in the rat brain and may participate in signaling and regulation of feeding behavior and longevity in the brain. PMID: 17720279
  19. 53BP2S interacts and modulates the insulin signals mediated by insulin receptor substrates. PMID: 17965023
  20. Data demonstrate that IRS4 interacts with the LR. This recruitment is leptin dependent and requires phosphorylation of the Y1077 motif of the LR. PMID: 18165436
  21. The association and altered regulation of IRS proteins by Ad5E1A contribute to the adenovirus-transformed phenotype and modulates viral infection in an Akt-dependent manner. PMID: 19029952

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Subcellular Location Cell membrane; Peripheral membrane protein; Cytoplasmic side.
Tissue Specificity Expressed in myoblasts. Expressed in liver and hepatocellular carcinoma.
Database Links

HGNC: 6128

OMIM: 300904

KEGG: hsa:8471

STRING: 9606.ENSP00000361202

UniGene: Hs.407141

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