Recombinant Human Integrin alpha-4(ITGA4) ,partial

Code CSB-YP011867HU
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Source Yeast
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Code CSB-EP011867HU
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Source E.coli
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Code CSB-EP011867HU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP011867HU
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Source Baculovirus
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Code CSB-MP011867HU
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names ITGA4
Uniprot No. P13612
Alternative Names 269C wild type; Antigen CD49D, alpha 4 subunit of VLA 4 receptor; CD49 antigen like family member D; CD49 antigen-like family member D; CD49d; IA4; Integrin alpha 4; Integrin alpha 4 subunit; Integrin alpha IV; Integrin alpha-4; Integrin alpha-IV; Integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA 4 receptor); ITA4_HUMAN; ITGA4; MGC90518; OTTHUMP00000205320; Very late activation protein 4 receptor, alpha 4 subunit; VLA 4 subunit alpha; VLA-4 subunit alpha; VLA4
Species Homo sapiens (Human)
Protein Length Partial
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
Integrins alpha-4/beta-1 (VLA-4) and alpha-4/beta-7 are receptors for fibronectin. They recognize one or more domains within the alternatively spliced CS-1 and CS-5 regions of fibronectin. They are also receptors for VCAM1. Integrin alpha-4/beta-1 recognizes the sequence Q-I-D-S in VCAM1. Integrin alpha-4/beta-7 is also a receptor for MADCAM1. It recognizes the sequence L-D-T in MADCAM1. On activated endothelial cells integrin VLA-4 triggers homotypic aggregation for most VLA-4-positive leukocyte cell lines. It may also participate in cytolytic T-cell interactions with target cells. ITGA4:ITGB1 binds to fractalkine (CX3CL1) and may act as its coreceptor in CX3CR1-dependent fractalkine signaling. ITGA4:ITGB1 binds to PLA2G2A via a site (site 2) which is distinct from the classical ligand-binding site (site 1) and this induces integrin conformational changes and enhanced ligand binding to site 1.
Gene References into Functions
  1. defines molecularly distinct subsets of articular cartilage that are enriched for osteochondral proteins PMID: 30194383
  2. study suggests a novel association of ITGA4 +3061A/G polymorphism with AD and its possible contribution to the disease pathology. PMID: 29769839
  3. The results of this study demonstrated that aggregation of hWJSCs into spherules alters their expression of ITGA4 and ITGA5. PMID: 29411210
  4. human ADAM11, ADAM23, and ADAM29 selectively support integrin alpha4-dependent cell adhesion. PMID: 28913673
  5. Recombinant human IL33 inhibited trophoblast invasion and adhesion, and decreased adhesion and invasionassociated molecules such as integrin alpha4beta1 and CD62L. PMID: 28765940
  6. study analyzes for the first time the expressions of epithelial-mesenchymal transition marker proteins - periostin, integrin alpha4, fibronectin - in metastatic castration-resistant prostate cancer PMID: 28836852
  7. ITGalpha4beta1 and TLR4 have roles in inducing fibrotic gene expression in response to fibronectin's EDA domain PMID: 28842322
  8. ITGA4 and PLCG1 are regulated by miR-30b in coronary artery endothelial cells from coronary atherosclerosis clinical samples. PMID: 27464494
  9. Low CD49D expression is associated with chronic lymphocytic leukaemia in comparison to small lymphocytic lymphoma. PMID: 29153094
  10. alpha-Integrin expression and function modulates presentation of cell surface calreticulin. PMID: 27310876
  11. AEbeta7 is of key relevance for gut trafficking of inflammatory bowel disease (IBD) CD8(+) T cells and CD4(+) Th9 cells in vivo and mainly retention might account for this effect. These findings indicate that blockade of alphaEbeta7 in addition to alpha4beta7 may be particularly effective in intestinal disorders with expansion of CD8(+) and Th9 cells such as IBD. PMID: 27543429
  12. sPLA2-IIA activates Integrin alphaVbeta3 and Integrin alpha4beta1 in an allosteric manner. (Review) PMID: 27864802
  13. Our results suggest that the analysis of alpha4beta7 integrin expression on circulating lymphocytes cannot be considered a reliable test for gastrointestinal involvement in CVID patients PMID: 28323147
  14. Glycosylation of V1V2 domain of HIV envelope protein determined its binding to alpha4ss7 integrin. PMID: 28577856
  15. confirmed CD49d as an independent negative OS prognosticator in CLL also in comprehensive models comprising the novel recurrent mutations. PMID: 27109509
  16. In colon cancer samples, there was a positive correlation between the expression of integrin alpha4 and VEGF-C. Integrin alpha4 and VEGF-C were significantly associated with the clinicopathological parameters (LMVD, Duke's stage, and lymph node metastasis). patients with high integrin alpha4 or VEGF-C expression had significantly shorter overall survival and tumor-free survival time. PMID: 26917449
  17. Macrophage tissue factor prothrombotic activity is regulated by integrin-alpha4/arf6 trafficking. PMID: 28495929
  18. gut-homing alpha4beta7 CD4(+) T cells and their functional subsets were profoundly depleted during acute HIV-1 infection. PMID: 26277899
  19. CD49d determination may be a useful tool to closely monitor Multiple Sclerosis activity in patients who interrupt Natalizumab PMID: 27496090
  20. Data show tht Rho kinase (ROCK) activity was increased by inhibiting integrin alpha4 with human umbilical vein endothelial cells (HUVECs) and hypoxia. PMID: 26448639
  21. These data showed that Mycobacterium tuberculosis ESAT-6 and ESAT-6/CFP-10 fusion proteins could induce adhesion of macrophages to fibronectin through alpha4beta1 integrin. PMID: 25081983
  22. This review gives an up to date insight into the multiple functional role of VLA-4 in cancer and introduces this integrin as a promising target worthwhile to attract attention in biomedical cancer research. PMID: 25564456
  23. Enhanced susceptibility of activated endocervical CD4+ T cells to HIV may be related to increased CCR5 expression by these cell subsets, but did not appear to be due to direct interaction of integrins a4b7 or a4b1 with HIV envelope. PMID: 25872482
  24. knockdown of PTTG1 increased expression of integrin alpha 4 (ITGA4), ITGA5, and integrin beta 1 (ITGB1); otherwise, RhoA expression was significantly decreased PMID: 26900962
  25. ITGA4 expression enhances metastasis in MYCN low neuroblastoma. PMID: 25973900
  26. Data show that a small molecule inhibitor of the MYC transcription factor can be an effective anticancer agent when delivered using a integrin alphavbeta3 and VLA-4-targeted nanotherapy approach. PMID: 25824336
  27. Collectively, these data suggest a role for alpha4beta7 integrin in HIV infection that is influenced by both viral and host factors including the sequence of the HIV gp120 alpha4beta7 binding motif, the cytokine milieu and bacterial vaginosis in the genital tract. PMID: 26105197
  28. Glioma cells induced the migration of BMSCs by promoting the expression of integrin a4. PMID: 26034878
  29. CD49d and CD26 have independent prognostic value and we suggest its use as a part of routine panel for prognostic stratification of CLL. PMID: 26142332
  30. Hypermethylation of ITGA4 promoter is increased in inflamed colon tissue. PMID: 25902909
  31. Regulatory CD4 T cells (Treg) cells in co-infected patients present phenotypic alterations and might have dysfunction marked by low expression of Foxp3 and increased expression of molecules not frequently seen on Treg cells, such as CD49d. PMID: 26329520
  32. High VLA-4 expression was independently associated with a high probability of complete remission in acute myeloid leukemia patients. PMID: 26155911
  33. Results show that integrin activation is absolutely required for OPN binding to the alpha4 integrin suggesting that OPN has very low affinity for the alpha4 integrin on human leukocytes under physiological conditions. PMID: 25446551
  34. alpha4beta7 serves as an attachment factor for some HIV-1 strains. PMID: 25527342
  35. Selective inhibition of VLA-4 expression on B cells impedes central nervous system accumulation of B cells and reduces susceptibility to experimental autoimmune encephalomyelitis. PMID: 25712734
  36. The carbon monoxide signaling pathway can rapidly down-modulate binding of the VLA-4 -specific ligand. PMID: 25367365
  37. CXCR4 and CD49d are important modulators of prognosis in chronic lymphocytic leukemia patients. PMID: 25349153
  38. Proinflammatory secreted phospholipase A2 type IIA (sPLA-IIA) induces integrin activation through direct binding to a newly identified binding site (site 2) in integrin alphaVbeta3, integrin alphavbeta1, and integrin alpha4beta1. PMID: 25398877
  39. LFA-1 and CR4 alpha chain phosphorylation is needed for chemokine-induced cross-talk to VLA-4 PMID: 25278023
  40. HIV-1 virions and many gp120s lack detectable alpha4beta7 binding activity. PMID: 25008916
  41. lymphocyte trafficking into the CNS under VLA-4 blockade can occur by using the alternative adhesion molecules, PSGL-1 and MCAM, the latter representing an exclusive pathway for TH17 cells to migrate over the blood-brain barrier PMID: 25135296
  42. DNA methylation of integrin alpha4 may be a poor prognostic factor which affects undifferentiated histologic change of breast cancer. PMID: 24756760
  43. Disruption of the hydrophobic contacts induces the active conformation of integrin alpha 4 beta 7. PMID: 24802248
  44. Pharmacological inhibition of the VLA-4/vascular adhesion molecule-1 axis in experimental stroke was ineffective PMID: 24743435
  45. Data indicate that CD47 in cis interactions regulate LFA-1 (integrin alphaLbeta2) and VLA-4 (integrin alpha4beta1) integrin affinity, and this process plays a substantial role in the adhesion of T-cells. PMID: 24006483
  46. VCAM-1/VLA-4-dependent activation of NF-kappaB has a role in mediating chemoresistance in leukemia cells PMID: 24599548
  47. Two conserved disulfide bonds located at integrin alpha4C589-C594 and beta7C494-C526 activated alpha4beta7. PMID: 23986478
  48. Only rotavirus specific CD4 T cells expressed intestinal homing receptors alpha4beta7 and CCR9. PMID: 24606696
  49. Data suggest CD38 (CD38 antigen (p45) protein) and CD49d (alpha4 Integrin; very late antigen-4 alpha) are more than just markers of an aggressive chronic lymphocytic leukemia (CLL) cell type and play functional roles in pathobiology of CLL. [REVIEW] PMID: 24288111
  50. Expression of the VLA-4-stimulating factor sequence may help to predict melanoma prometastatic risk. PMID: 23938462

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Subcellular Location Membrane; Single-pass type I membrane protein.
Protein Families Integrin alpha chain family
Database Links

HGNC: 6140

OMIM: 192975

KEGG: hsa:3676

STRING: 9606.ENSP00000380227

UniGene: Hs.440955

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