Code | CSB-RA225859A0HU |
Size | US$210 |
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Application | Recommended Dilution |
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WB | 1:500-1:5000 |
The ITGA4 recombinant monoclonal antibody is produced using recombinant DNA technology and has been validated for use in the detection of human ITGA4 protein in ELISA and WB applications. First, myeloma cells and B cells from an animal immunized with a synthesized peptide derived from human ITGA4 are fused to produce the hybridomas. The cDNA of the ITGA4 antibody-producing hybridomas is then sequenced, and the gene coding for the ITGA4 monoclonal antibody is synthesized. The synthesized gene is then cloned into a vector and transfected into cells for cultivation. Finally, the ITGA4 recombinant monoclonal antibody is purified from the cell culture supernatant using affinity chromatography.
The ITGA4 protein is expressed on the surface of various cell types, including leukocytes, and is involved in regulating cell-to-cell interactions, cell adhesion, cell signaling, and migration. In particular, ITGA4 functions as a receptor for VCAM-1, the binding of which helps to mediate the adhesion and migration of leukocytes across the endothelium and into tissues during immune responses. ITGA4 has also been implicated in regulating cell proliferation, survival, and differentiation. Dysregulation of ITGA4 has been associated with various diseases, including inflammatory and autoimmune disorders, cancer, and cardiovascular disease.
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