Recombinant Human Iodotyrosine dehalogenase 1 (IYD), partial

Code CSB-YP754272HU
MSDS
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Source Yeast
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Code CSB-EP754272HU
MSDS
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Source E.coli
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Code CSB-EP754272HU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP754272HU
MSDS
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Source Baculovirus
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Code CSB-MP754272HU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
IYD
Uniprot No.
Alternative Names
IYD; C6orf71; DEHAL1; Iodotyrosine deiodinase 1; IYD-1; EC 1.21.1.1; Iodotyrosine dehalogenase 1
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Catalyzes the oxidative NADPH-dependent deiodination of monoiodotyrosine (L-MIT) or diiodotyrosine (L-DIT). Acts during the hydrolysis of thyroglobulin to liberate iodide, which can then reenter the hormone-producing pathways. Acts more efficiently on monoiodotyrosine than on diiodotyrosine.
Gene References into Functions
  1. The rate-limiting processes that contribute to the ability of flavin to promote reductive dehalogenation in human IYD. PMID: 26151430
  2. A switch between one- and two-electron chemistry of iodotyrosine deiodinase is controlled by substrate. PMID: 25395621
  3. Iodotyrosine deiodinase defect identified via genome-wide approach. PMID: 22535972
  4. high activity of human saliva peroxidase with iodide as a substrate may play a crucial role in the bioavailability and metabolism of biologically active iodide. PMID: 21870604
  5. Mutations in DEHAL1 leads to hypothyroidism, goiter and mental retardation (Review) PMID: 20298747
  6. molecular cloning and investigation of the localization and activity of DEHAL1 PMID: 15289438
  7. the cytoplasmic tail of DEHAL1 could play a role in the stability of the protein PMID: 16910871
  8. Diffuse cytoplasmatic localisation or downregulation of DEHAL1 expression in thyroid cancers suggests alteration or loss of DEHAL1 function during thyroid cell dedifferentiation. PMID: 17322488
  9. homozygous mutations in DEHAL1 appear to cause human iodotyrosine deiodinase deficiency, leading to hereditary hypothyroidism and goiter PMID: 18434651
  10. This study describes a functional mutation within IYD, demonstrating the molecular basis of the iodine wasting form of congenital hypothyroidism PMID: 18765512

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Involvement in disease
Thyroid dyshormonogenesis 4 (TDH4)
Subcellular Location
Cell membrane; Single-pass membrane protein. Cytoplasmic vesicle membrane.
Protein Families
Nitroreductase family
Tissue Specificity
Expressed at a high level in thyroid gland (at protein level). Expressed at a high level in thyroid gland and at lower level in kidney and trachea.
Database Links

HGNC: 21071

OMIM: 274800

KEGG: hsa:389434

STRING: 9606.ENSP00000229447

UniGene: Hs.310225

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