Recombinant Human Keratin,type II cytoskeletal 8(KRT8)

Code CSB-EP012574HU(A4)
Size US$1726
Image
  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity Greater than 85% as determined by SDS-PAGE.
Target Names KRT8
Uniprot No. P05787
Research Area Signal Transduction
Alternative Names
CARD2; CK 8; CK-8; CK8; CYK8; CYKER; Cytokeratin endo A; Cytokeratin-8; DreK8; EndoA; K2C8; K2C8_HUMAN; K8; Keratin 8; Keratin type II cytoskeletal 8 ; Keratin; type II cytoskeletal 8; Keratin-8; KO; Krt 2.8; KRT8; MGC118110; MGC174782; MGC53564; MGC85764; sb:cb186; Type-II keratin Kb8
Species Homo sapiens (Human)
Source E.coli
Expression Region 1-483aa
Target Protein Sequence MSIRVTQKSYKVSTSGPRAFSSRSYTSGPGSRISSSSFSRVGSSNFRGGLGGGYGGASGMGGITAVTVNQSLLSPLVLEVDPNIQAVRTQEKEQIKTLNNKFASFIDKVRFLEQQNKMLETKWSLLQQQKTARSNMDNMFESYINNLRRQLETLGQEKLKLEAELGNMQGLVEDFKNKYEDEINKRTEMENEFVLIKKDVDEAYMNKVELESRLEGLTDEINFLRQLYEEEIRELQSQISDTSVVLSMDNSRSLDMDSIIAEVKAQYEDIANRSRAEAESMYQIKYEELQSLAGKHGDDLRRTKTEISEMNRNISRLQAEIEGLKGQRASLEAAIADAEQRGELAIKDANAKLSELEAALQRAKQDMARQLREYQELMNVKLALDIEIATYRKLLEGEESRLESGMQNMSIHTKTTSGYAGGLSSAYGGLTSPGLSYSLGSSFGSGAGSSSFSRTSSSRAVVVKKIETRDGKLVSESSDVLPK
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight 57.8 kDa
Protein Length Full length
Tag Info N-terminal 6xHis-tagged
Form Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer Tris-based buffer,50% glycerol
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA Please contact us to get it.

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Target Background

Function
Together with KRT19, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.
Gene References into Functions
  1. KRT8 mRNA and protein are upregulated in anaplastic thyroid carcinoma. KRT8 protein binds to annexin A2. PMID: 29443941
  2. K8/K18 variants are overrepresented in Chinese NAFLD patients and might accelerate liver fat storage through insulin resistance. PMID: 28652657
  3. regulated enhancement of autophagy with concurrent increased expression of KRT8 and the inhibition of KRT8 phosphorylation serve to inhibit oxidative stress-induced epithelial-mesenchymal transitionof retinal pigment epithelium cells. PMID: 28045574
  4. Histopathological differences between Magnetic resonance-guided high-intensity focused ultrasound (MR-HIFU)-ablated tissue and radiofrequency (RF)-ablated tissue were demonstrated. CK-8 was more reliable for cell viability assessment than H&E when used directly after ablation PMID: 26732321
  5. Loss of activating transcription factor 3 (ATF3) in knockout mice promotes the emergence of keratins CK5+CK8+ epithelial cells. PMID: 26522727
  6. notochord-specific marker during early intervertebral disc development PMID: 26910849
  7. Data show that the filament elongation of both desmin and keratin K8/K18 proceeds very similar to that of vimentin. PMID: 27304995
  8. New insights into interactions between the nucleotide-binding domain of CFTR and keratin 8. PMID: 27870250
  9. We present results of a multicentre study measuring UBC((R)) Rapid Test(test that detects fragments of cytokeratins 8 and 18 in urine) in bladder cancer patients and healthy controls with focus on carcinoma in situ (CIS) and high-grade bladder cancer. PMID: 28468590
  10. Data show that after siRNA transfection, TGF-beta1 protein level was decreased, and CK18 proteins was decreased, while CK8 proteins was increased, and TERT protein expression was slightly increased at 96 h. PMID: 26462837
  11. The interplay between Solo protein and keratin 8/keratin 18 filaments plays a crucial role in tensile force-induced RhoA activation and consequent actin cytoskeletal reinforcement in endothelial cells. PMID: 26823019
  12. K8/18 filaments provide resistance to apoptosis in granulosa cell tumor cells by impairing FAS expression. PMID: 26911253
  13. These metastatic tumors revealed no detectable expression of CK8/18, E-cadherin, VCAM-1, and ICAM-1 PMID: 26847082
  14. Data show that loss of epithelial membrane protein 2 (EMP2) is involved in sphingosylphosphorylcholine (SPC)-induced phosphorylation of keratin 8 (K8) via ubiquitination of protein phosphatase 2 (PP2A) through alpha4 phosphoprotein by caveolin-1 (cav-1). PMID: 26876307
  15. The reactivity of rheumatoid arthritis (RA) serum IgG antibodies against K8 was significantly higher than healthy controls and systemic lupus erythematosus patients, confirming K8 as a novel autoantigen of RA. PMID: 26277397
  16. Variants in highly conserved residues of K8 Lys393Arg (ezetimibe/simvastatin-related) was found in patients with fatal Drug-induced liver injury. PMID: 26286715
  17. Keratins 8 and 18 are type II acute-phase responsive genes overexpressed in human liver disease. PMID: 24930437
  18. In human failing myocardium, where TNF-alpha expression is upregulated, K8/K18 were also ectopically expressed PMID: 26280121
  19. Human KRT8 variants promote acetaminophen induced hepatotoxicity in mouse models. PMID: 25963979
  20. Syndecan-1 is involved in morphogenesis of the developing tooth crown and cervical loops, andntogether with CK8 and vimentin in differentiation of preameloblasts and preodontoblasts. PMID: 25120060
  21. K8 immunostaining of tissue from the lymph node metastasis allows defining a sub-group of lymph node-positive breast cancer patients with a highly unfavourable outcome PMID: 25724181
  22. This is the first time intestinal cells have been suggested also functionally impaired by K8/K18 mutations. PMID: 24915158
  23. Study indicates that Piwil2 plays a role in Fas-mediated apoptosis and can affect p53 phosphorylation in tumor cells, revealing a novel mechanism of Piwil2 in apoptosis, and supports that Piwil2 plays an active role in tumorigenesis. PMID: 25113562
  24. An increased expression of cytokeratin 8 and weak of calponin in tunica media of saphenous vein grafts might be useful markers of unfavorable long-term prognosis in CABG patients. PMID: 24338534
  25. These data support the idea that CK8 is an important receptor for Pet on epithelial cells for starting its cytotoxic effects. PMID: 24327340
  26. parkin may inhibit cell growth, at least in part, by decreasing RPSA expression and inducing phosphorylation of cytokeratin 8/18 PMID: 23990477
  27. These findings, along with the emerging role of cell surface CK8 in cancer metastasis, may imply a dual strategy for tumor cell survival in the host. PMID: 24183726
  28. CK8 up-regulation might have a functional role in HCV infection and pathogenesis, and its ectopic expression induces apoptosis of SMMC7721 cells. PMID: 24115814
  29. this is the first study to show a down-regulated CK8 expression and the percentage of CK8 positive cell clusters in IDD based upon multiple lines of evidence PMID: 23801880
  30. Compressive loads resulted in phosphorylation and disassembly of CK8 in nucleus pulposus cells. PMID: 24166186
  31. A conserved rod domain phosphotyrosine that is targeted by the phosphatase PTP1B promotes keratin 8 protein insolubility and filament organization. PMID: 24003221
  32. These results indicate the role of K8/18 in modulating invasion in breast cancer. PMID: 23341946
  33. Report keratin 8 expression in breast papillomas and papillary carcinoma. PMID: 23327593
  34. K8/18 expression can influence the phenotype of epithelial cancer cells at a transcriptional level and supports the hypothesis that keratins play an active role in cancer progression. PMID: 23449973
  35. Keratin variants are expressed in a low percentage of patients with alcoholic cirrhosis and do not influence hepatocellular carcinoma development. PMID: 23078008
  36. There is aberrant expression of CK8 in dysplasia, oral submucous fibrosis, and oral squamous cell carcinoma, suggesting CK8 as a surrogate marker of malignant transformation. PMID: 22677743
  37. K8 acetylation at Lys-207, a highly conserved residue among type II keratins, is up-regulated upon hyperglycemia and down-regulated by SIRT2. PMID: 23358244
  38. A key K8/K18 regulation of glucose metabolism in normal and cancerous hepatic cells through differential modulations of mitochondrial hexokinase status and insulin-mediated signaling. PMID: 23164509
  39. Fibroblasts prepared from different types of malignant tumors stimulate expression of luminal marker keratin 8. PMID: 22270320
  40. Keratin filaments were assembled from recombinant keratin proteins 8 (K8) and 18 (K18) in the presence of divalent ions (Mg(2+)). PMID: 22853896
  41. Keratin 8 phosphorylation regulates keratin reorganization and migration of epithelial tumor cells. PMID: 22344252
  42. Results show K8 is present on the cell surface of transformed prostate cancer cell lines, and K8 increased the activity of plasmin approximately fivefold over a 48-h period. K8 also enhanced the plasmin-mediated proteolysis of vitronectin. PMID: 22354665
  43. In patients with hemochromatosis, intronic but not exonic K8/K18 variants associate with liver fibrosis development. PMID: 22412904
  44. We have found the moderate positive correlation between proliferation and a keratin 8 expression in neoplastic epithelial cells. PMID: 22379894
  45. previously undescribed K8 variants associate with cryptogenic hepatitis in a German cohort of patients, possibly predisposing carriers to the development of liver disease. PMID: 22419260
  46. Loss of keratin 8 phosphorylation leads to increased oral squamous cell carcinoma progression PMID: 22114688
  47. Suggest that CK8 is unsuitable for early diagnosis of tongue squamous cell carcinoma. PMID: 21705376
  48. the mild KRT8 allele is associated with CFTR mediated residual chloride secretion among F508del-CFTR homozygotes PMID: 21548936
  49. The study demonstrates the strong expression of CK8 in limbal epithelial basal cells, which is maintained during the differentiation and migration of the limbal cells toward the central corneal epithelium. PMID: 20926822
  50. Clumping factor B is capable of binding cytokeratin 8 during infection. PMID: 20817646

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Involvement in disease Cirrhosis (CIRRH)
Subcellular Location Cytoplasm. Nucleus, nucleoplasm. Nucleus matrix.
Protein Families Intermediate filament family
Tissue Specificity Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma membrane in structures that contain dystrophin and spectrin. Expressed in gingival mucosa and hard palate of the oral cavity.
Database Links

HGNC: 6446

OMIM: 148060

KEGG: hsa:3856

STRING: 9606.ENSP00000293308

UniGene: Hs.533782

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