Recombinant Human Kinesin-like protein KIF14 (KIF14), partial

Code CSB-YP613582HU
MSDS
Size Pls inquire
Source Yeast
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-EP613582HU
MSDS
Size Pls inquire
Source E.coli
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-EP613582HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-BP613582HU
MSDS
Size Pls inquire
Source Baculovirus
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-MP613582HU
MSDS
Size Pls inquire
Source Mammalian cell
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Purity
>85% (SDS-PAGE)
Target Names
KIF14
Uniprot No.
Alternative Names
KIAA0042; KIF 14; KIF14; KIF14_HUMAN; Kinesin Family Member 14; Kinesin like protein KIF14; Kinesin-like protein KIF14; MGC142302
Species
Homo sapiens (Human)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function
Microtubule motor protein that binds to microtubules with high affinity through each tubulin heterodimer and has an ATPase activity. Plays a role in many processes like cell division, cytokinesis and also in cell proliferation and apoptosis. During cytokinesis, targets to central spindle and midbody through its interaction with PRC1 and CIT respectively. Regulates cell growth through regulation of cell cycle progression and cytokinesis. During cell cycle progression acts through SCF-dependent proteasomal ubiquitin-dependent protein catabolic process which controls CDKN1B degradation, resulting in positive regulation of cyclins, including CCNE1, CCND1 and CCNB1. During late neurogenesis, regulates the cerebellar, cerebral cortex and olfactory bulb development through regulation of apoptosis, cell proliferation and cell division. Also is required for chromosome congression and alignment during mitotic cell cycle process. Regulates cell spreading, focal adhesion dynamics, and cell migration through its interaction with RADIL resulting in regulation of RAP1A-mediated inside-out integrin activation by tethering RADIL on microtubules.
Gene References into Functions
  1. KIF14 missense and loss of function variants were identified in intellectual disability with microcephaly patients. PMID: 29343805
  2. KIF14 is upregulated in PCa cell lines and clinical PCa tissues. Inhibition of KIF14 suppresses cell proliferation, induces G2 arrest and apoptosis. PMID: 28525372
  3. In particular, counteracting forces between minus-end-directed kinesin-14 and plus-end-directed kinesin-5 motors have recently been implicated in the regulation of microtubule nucleation. PMID: 28668932
  4. The mechanochemical cycle of kinesin-14 has been reported. PMID: 27729532
  5. Data indicate that three genes, KIF14, NCAPG and CENPE that were upregulated in Pediatric high-grade gliomas (pHGGs) and were direct miR-137 or miR-6500-3p targets. PMID: 26933822
  6. This study identified the mutations in KIF14 as a new cause of primary microcephaly and using cellular experiments demonstrate that impaired cytokinesis, increased apoptosis, and reduced cell motility are features of the associated pathogenesis. PMID: 28892560
  7. Signaling cascade of the NIMA-related kinases (Neks) Nek6, Nek7, and Nek9 is required for the localization and function of two kinesins essential for cytokinesis, Mklp2 and Kif14 to properly coordinate cytokinesis. PMID: 28630147
  8. Studied overexpression of KIF14 which showed the tight correlation between KIF14 upregulation and inferior clinical outcome in medulloblastoma (MB), suggesting KIF14 is a potential negative prognostic marker in MB. Transient, siRNAs-mediated downregulation of KIF14 suppressed cell proliferation and induced apoptosis in two MB cell lines. PMID: 28504687
  9. study suggests that KIF14 may serve as a predictor of poor survival and a novel prognostic biomarker of chemoresistance to paclitaxel treatment in cervical cancer. PMID: 27128470
  10. analysis of epigenetic regulation of KIF14 overexpression in ovarian cancer PMID: 24626475
  11. Kinesin-14 blocks microtubule nucleation in yeast and reveal that this inhibition is countered by the kinesin-5 protein, Cut7.[Cut7, Pkl1] PMID: 25348260
  12. High-grade serous ovarian cancer cells may depend on KIF14 for in vitro proliferation. PMID: 25528264
  13. Mutations in KIF14 identified as a novel cause of an autosomal recessive lethal fetal ciliopathy phenotype. PMID: 24128419
  14. critical role for KIF14 in the tumorigenic potential of triple-negative breast cancer PMID: 24784001
  15. High KIF14 expression is associated with hepatocellular carcinoma. PMID: 25106407
  16. KIF14 knockdown downregulates the expression of Skp2 and Cks1, leading to accumulation of p27Kip1. PMID: 24854087
  17. KIF14 inhibits tumor growth and cancer metastasis in lung adenocarcinoma. PMID: 23626713
  18. Data indicate that KIF14 and TLN1 loss-of-function significantly enhanced chemosensitivity in four triple-negative breast cancer (TNBC) cell lines. PMID: 23479679
  19. Suppression of KIF14 not only decreases cancer cell migration but also induces apoptosis of cells. PMID: 23414349
  20. KIF14 expression in gliomas is tumor-specific and increased in more aggressive tumors. KIF14 might function as a candidate prognostic marker for human gliomas. PMID: 22999822
  21. It was shown that the kinesin KIF14 associates with the PDZ domain of Radil and negatively regulates Rap1-mediated inside-out integrin activation by tethering Radil on microtubules. PMID: 23209302
  22. Findings indicate that KIF14 mRNA is an independent prognostic marker in serous ovarian cancer. PMID: 21618518
  23. Observational study and genome-wide association study of gene-disease association. (HuGE Navigator) PMID: 20306291
  24. Primary non-small-cell lung carcinoma overexpressing KIF14 showed a trend toward decreased survival. PMID: 15897902
  25. During cytokinesis, the localization of KIF14 and citron kinase to the central spindle and midbody is codependent, and they form a complex depending on the activation state of citron kinase. PMID: 16431929
  26. RNA interference-mediated silencing of KIF14 disrupts cell cycle progression and induces cytokinesis failure. PMID: 16648480
  27. KIF14 is expressed most highly in squamous cell carcinoma, then in large-cell undifferentiated carcinoma, then adenocarcinoma PMID: 17545527
  28. Overexpression of KIF14 was confirmed in primary human retinoblastoma and showed that patients with an older age at diagnosis express significantly higher levels of KIF14. PMID: 17962437
  29. KIF14 overexpression is associated with papillary renal cell tumors with chromosome 1q duplication. PMID: 19123481
  30. report confirms significant mRNA overexpression of KIF14 and E2F3 together in a large cohort of retinoblastoma tumors PMID: 19190782
  31. Results show that the established nerve invasion model and the consensus signature of perineural invasion could be instrumental in the identification of novel therapeutic targets of pancreatic cancer as exemplified by KIF14 and ARHGDIbeta. PMID: 19509238
  32. Four genes previously not examined in that respect in laryngeal carcinoma, occurred to be good markers of the neoplasm. They are: metal-proteinase ADAM12, cyclin-dependent kinase 2-CDK2, kinesin 14-KIF14, suppressor 1 of checkpoint-CHES1. PMID: 19609547
  33. KIF14 mRNA expression is prognostic for disease-free survival in non-small-cell lung cancer. Knockdown inhibits colony formation ability. PMID: 17545527
  34. The KIF14 gene is amplified in retinoblastoma; likely an early genomic change in this cancer. PMID: 17099872
  35. KIF14 is overexpressed in primary breast cancer; predicts disease-free and overall survival PMID: 16570270
  36. Overexpressed in primary retinoblastoma and lung cancer, and in medulloblastoma and breast cancer cell lines. Low expression in normal adult tissue; highly expressed in fetal liver and thymus. PMID: 15897902

Show More

Hide All

Involvement in disease
Meckel syndrome 12 (MKS12)
Subcellular Location
Nucleus. Cytoplasm. Cytoplasm, cytoskeleton, spindle. Midbody.
Protein Families
TRAFAC class myosin-kinesin ATPase superfamily, Kinesin family
Database Links

HGNC: 19181

OMIM: 611279

KEGG: hsa:9928

STRING: 9606.ENSP00000356319

UniGene: Hs.3104

icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2024 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1