Recombinant Human Kinetochore protein Spc25 (SPC25)

Code CSB-YP881019HU
MSDS
Size Pls inquire
Source Yeast
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-EP881019HU
MSDS
Size Pls inquire
Source E.coli
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-EP881019HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-BP881019HU
MSDS
Size Pls inquire
Source Baculovirus
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-MP881019HU
MSDS
Size Pls inquire
Source Mammalian cell
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Purity
>85% (SDS-PAGE)
Target Names
SPC25
Uniprot No.
Alternative Names
AD024; hSpc25; Kinetochore protein Spc25; SPBC25; SPC25; SPC25_HUMAN; spindle pole body component 25 homolog
Species
Homo sapiens (Human)
Expression Region
1-224
Target Protein Sequence
MVEDELALFD KSINEFWNKF KSTDTSCQMA GLRDTYKDSI KAFAEKLSVK LKEEERMVEM FLEYQNQISR QNKLIQEKKD NLLKLIAEVK GKKQELEVLT ANIQDLKEEY SRKKETISTA NKANAERLKR LQKSADLYKD RLGLEIRKIY GEKLQFIFTN IDPKNPESPF MFSLHLNEAR DYEVSDSAPH LEGLAEFQEN VRKTNNFSAF LANVRKAFTA TVYN
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function
Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules.
Gene References into Functions
  1. SPC25 overexpression significantly increased the cancer stem cell properties and invasion of A549 cells. PMID: 29432994
  2. SPC25 expressed by extracellular matrix stiffening is required for lung cancer cell proliferation. PMID: 29709477
  3. SPC25 overexpression is associated with Breast Cancer. PMID: 27197203
  4. hSPC25 is an essential kinetochore component that plays a significant role in proper execution of mitotic events PMID: 14699129
  5. the Spc24, Spc25, Nuf2, and Ndc80/Hec1 complex is a faithful copy of the endogenous Ndc80 complex PMID: 15961401

Show More

Hide All

Subcellular Location
Nucleus. Chromosome, centromere, kinetochore.
Protein Families
SPC25 family
Database Links

HGNC: 24031

OMIM: 609395

KEGG: hsa:57405

STRING: 9606.ENSP00000282074

UniGene: Hs.421956

icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2024 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1