Recombinant Human Kynurenine 3-monooxygenase (KMO)

Code CSB-YP012475HU
MSDS
Size $250
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details

Purity
Greater than 90% as determined by SDS-PAGE.
Target Names
KMO
Uniprot No.
Research Area
Metabolism
Alternative Names
dJ317G22.1; kmo; KMO_HUMAN; Kynurenine 3 hydroxylase; Kynurenine 3 monooxygenase (kynurenine 3 hydroxylase); Kynurenine 3 monooxygenase; Kynurenine 3-hydroxylase; Kynurenine 3-monooxygenase; RP1-317G22.1
Species
Homo sapiens (Human)
Source
Yeast
Expression Region
1-486aa
Target Protein Sequence
MDSSVIQRKKVAVIGGGLVGSLQACFLAKRNFQIDVYEAREDTRVATFTRGRSINLALSHRGRQALKAVGLEDQIVSQGIPMRARMIHSLSGKKSAIPYGTKSQYILSVSRENLNKDLLTAAEKYPNVKMHFNHRLLKCNPEEGMITVLGSDKVPKDVTCDLIVGCDGAYSTVRSHLMKKPRFDYSQQYIPHGYMELTIPPKNGDYAMEPNYLHIWPRNTFMMIALPNMNKSFTCTLFMPFEEFEKLLTSNDVVDFFQKYFPDAIPLIGEKLLVQDFFLLPAQPMISVKCSSFHFKSHCVLLGDAAHAIVPFFGQGMNAGFEDCLVFDELMDKFSNDLSLCLPVFSRLRIPDDHAISDLSMYNYIEMRAHVNSSWFIFQKNMERFLHAIMPSTFIPLYTMVTFSRIRYHEAVQRWHWQKKVINKGLFFLGSLIAISSTYLLIHYMSPRSFLRLRRPWNWIAHFRNTTCFPAKAVDSLEQISNLISR
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
57.8kDa
Protein Length
Full Length
Tag Info
N-terminal 6xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Catalyzes the hydroxylation of L-kynurenine (L-Kyn) to form 3-hydroxy-L-kynurenine (L-3OHKyn). Required for synthesis of quinolinic acid, a neurotoxic NMDA receptor antagonist and potential endogenous inhibitor of NMDA receptor signaling in axonal targeting, synaptogenesis and apoptosis during brain development. Quinolinic acid may also affect NMDA receptor signaling in pancreatic beta cells, osteoblasts, myocardial cells, and the gastrointestinal tract (Probable).
Gene References into Functions
  1. There is the association between the rs1053230 polymorphism and depression. PMID: 28139632
  2. results show the incidence of Postpartum Depression in the Chinese population to be 7.3%, with PDS characterized by increased serum 3-HydroxyKinurenine concentration and 3-HK/Kynurenine ratio, versus matched postpartum women without PDS. Furthermore, polymorphisms of Kynurenine Monooxygenase rs1053230 are significantly associated with the incidence of PDS. PMID: 28319697
  3. Our data establish the candidacy of KMO as a causal factor for changes in the kidney leading to proteinuria and indicate a functional role for KMO and metabolites of the tryptophan pathway in podocytes PMID: 27020856
  4. A comprehensive review of the molecular properties of KMO, including its kinetics, reaction mechanism, and inhibitor structure-activity relationship (SAR), is not currently available and, thus, is our focus here PMID: 26589832
  5. SiRNA knockdown of the pathway components Kynurenine 3-monooxygenase and quinolinate phosphoribosyl transferase caused cells to revert to a state of susceptibility to 3HK-mediated apoptosis. PMID: 27077813
  6. These results suggest that KMO exhibits tumor-promoting effects towards hepatocellular carcinoma (HCC) and it may serve as a novel prognostic marker in HCC. PMID: 26099564
  7. Results suggest that KMO variation influences a range of cognitive domains known to predict functional outcome in schizophrenia PMID: 25464917
  8. Two KMO SNPs were observed more often in schizophrenia patient group compared with healthy controls. PMID: 25715464
  9. findings from five independent cohorts suggest that genetic variation in KMO influences the risk for psychotic features in mania of bipolar disorder patients. PMID: 23459468
  10. Study reporting on the first successful bacterial (Escherichia coli) expression of active FLAGtrade mark-tagged human KMO enzyme expressed in the soluble fraction and progress towards its purification. PMID: 24316190
  11. analyzed association between KMO gene polymorphisms and CSF concentrations of kynurenic acid in patients with schizophrenia and controls. results suggest that the nonsynonymous KMO SNP rs1053230 influences CSF concentrations of KYNA. PMID: 21693093
  12. significant and correlated reduction in gene expression and enzyme activity in the frontal eye field in schizophrenia patients; rs2275163 has modest effects on predictive pursuit and visuospatial working memory endophenotypes PMID: 21727251
  13. The present analysis of the combined Scandinavian sample did not reveal any allele frequency difference between patients and healthy controls PMID: 21030213
  14. the function KMO AND indoleamine 2,3-dioxygenase may change from a role in immunosuppression at the maternal-fetal interface in early pregnancy, to one associated with regulation of fetoplacental blood flow or placental metabolism in late gestation PMID: 15950064
  15. Results suggest that kynurenine 3-monooxygenase is unlikely to be related to the development of schizophrenia in Japanese. PMID: 16716206

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Subcellular Location
Mitochondrion outer membrane; Multi-pass membrane protein.
Protein Families
Aromatic-ring hydroxylase family, KMO subfamily
Tissue Specificity
Highest levels in placenta and liver. Detectable in kidney.
Database Links

HGNC: 6381

OMIM: 603538

KEGG: hsa:8564

STRING: 9606.ENSP00000355517

UniGene: Hs.731056

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