Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

KMO

Uniprot No.

O15229

Research Area

Metabolism

Alternative Names

dJ317G22.1; kmo; KMO_HUMAN; Kynurenine 3 hydroxylase; Kynurenine 3 monooxygenase (kynurenine 3 hydroxylase); Kynurenine 3 monooxygenase; Kynurenine 3-hydroxylase; Kynurenine 3-monooxygenase; RP1-317G22.1

Species

Homo sapiens (Human)

Source

E.coli

Expression Region

1-486aa

Target Protein Sequence

MDSSVIQRKKVAVIGGGLVGSLQACFLAKRNFQIDVYEAREDTRVATFTRGRSINLALSHRGRQALKAVGLEDQIVSQGIPMRARMIHSLSGKKSAIPYGTKSQYILSVSRENLNKDLLTAAEKYPNVKMHFNHRLLKCNPEEGMITVLGSDKVPKDVTCDLIVGCDGAYSTVRSHLMKKPRFDYSQQYIPHGYMELTIPPKNGDYAMEPNYLHIWPRNTFMMIALPNMNKSFTCTLFMPFEEFEKLLTSNDVVDFFQKYFPDAIPLIGEKLLVQDFFLLPAQPMISVKCSSFHFKSHCVLLGDAAHAIVPFFGQGMNAGFEDCLVFDELMDKFSNDLSLCLPVFSRLRIPDDHAISDLSMYNYIEMRAHVNSSWFIFQKNMERFLHAIMPSTFIPLYTMVTFSRIRYHEAVQRWHWQKKVINKGLFFLGSLIAISSTYLLIHYMSPRSFLRLRRPWNWIAHFRNTTCFPAKAVDSLEQISNLISR
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

71.8kDa

Protein Length

Full Length

Tag Info

N-terminal 6xHis-SUMO-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The preparation of Recombinant Human KMO protein included 3 main steps: construct the expression vector, expression of protein of interest, and protein purification. Every step was performed under a strict QC system so that we got the premium protein. This KMO was expressed in E.coli at and fused with N-terminal 6xHis-SUMO tag. According to SDS-PAGE, the purity turns out to be 90%+.

kynurenine 3-monooxygenase (KMO) functions as a pivotal enzyme in the main branch of the enzymatic cascade because it generates several toxic metabolites, including 3-hydroxykynurenine and quinolinic acid, which are responsible for neurodegenerative and inflammatory disorders. In recent years, researchers first revealed a novel enzymatic activity independent function of KMO in triple negative breast cancer (TNBC). Specifically, the findings suggested a positive correlation between the increased expression of KMO in breast cancer and TNBC and metastasis and recurrence. Besides, the contribution of KMO expression to the malignant phenotype of TNBC cells, particularly cancer stem cell (CSC) properties, but which leads to increased expression of pluripotent genes such as Nanog, Oct4, and Sox2.

Target Background

Function

Catalyzes the hydroxylation of L-kynurenine (L-Kyn) to form 3-hydroxy-L-kynurenine (L-3OHKyn). Required for synthesis of quinolinic acid, a neurotoxic NMDA receptor antagonist and potential endogenous inhibitor of NMDA receptor signaling in axonal targeting, synaptogenesis and apoptosis during brain development. Quinolinic acid may also affect NMDA receptor signaling in pancreatic beta cells, osteoblasts, myocardial cells, and the gastrointestinal tract (Probable).

Gene References into Functions

There is the association between the rs1053230 polymorphism and depression. PMID: 28139632
results show the incidence of Postpartum Depression in the Chinese population to be 7.3%, with PDS characterized by increased serum 3-HydroxyKinurenine concentration and 3-HK/Kynurenine ratio, versus matched postpartum women without PDS. Furthermore, polymorphisms of Kynurenine Monooxygenase rs1053230 are significantly associated with the incidence of PDS. PMID: 28319697
Our data establish the candidacy of KMO as a causal factor for changes in the kidney leading to proteinuria and indicate a functional role for KMO and metabolites of the tryptophan pathway in podocytes PMID: 27020856
A comprehensive review of the molecular properties of KMO, including its kinetics, reaction mechanism, and inhibitor structure-activity relationship (SAR), is not currently available and, thus, is our focus here PMID: 26589832
SiRNA knockdown of the pathway components Kynurenine 3-monooxygenase and quinolinate phosphoribosyl transferase caused cells to revert to a state of susceptibility to 3HK-mediated apoptosis. PMID: 27077813
These results suggest that KMO exhibits tumor-promoting effects towards hepatocellular carcinoma (HCC) and it may serve as a novel prognostic marker in HCC. PMID: 26099564
Results suggest that KMO variation influences a range of cognitive domains known to predict functional outcome in schizophrenia PMID: 25464917
Two KMO SNPs were observed more often in schizophrenia patient group compared with healthy controls. PMID: 25715464
findings from five independent cohorts suggest that genetic variation in KMO influences the risk for psychotic features in mania of bipolar disorder patients. PMID: 23459468
Study reporting on the first successful bacterial (Escherichia coli) expression of active FLAGtrade mark-tagged human KMO enzyme expressed in the soluble fraction and progress towards its purification. PMID: 24316190
analyzed association between KMO gene polymorphisms and CSF concentrations of kynurenic acid in patients with schizophrenia and controls. results suggest that the nonsynonymous KMO SNP rs1053230 influences CSF concentrations of KYNA. PMID: 21693093
significant and correlated reduction in gene expression and enzyme activity in the frontal eye field in schizophrenia patients; rs2275163 has modest effects on predictive pursuit and visuospatial working memory endophenotypes PMID: 21727251
The present analysis of the combined Scandinavian sample did not reveal any allele frequency difference between patients and healthy controls PMID: 21030213
the function KMO AND indoleamine 2,3-dioxygenase may change from a role in immunosuppression at the maternal-fetal interface in early pregnancy, to one associated with regulation of fetoplacental blood flow or placental metabolism in late gestation PMID: 15950064
Results suggest that kynurenine 3-monooxygenase is unlikely to be related to the development of schizophrenia in Japanese. PMID: 16716206

Hide All

Subcellular Location

Mitochondrion outer membrane; Multi-pass membrane protein.

Protein Families

Aromatic-ring hydroxylase family, KMO subfamily

Tissue Specificity

Highest levels in placenta and liver. Detectable in kidney.

Database Links

HGNC: 6381

OMIM: 603538

KEGG: hsa:8564

STRING: 9606.ENSP00000355517

UniGene: Hs.731056

Code	CSB-EP012475HU
Abbreviation	Recombinant Human KMO protein
MSDS	MSDS Datasheet
Size	$224
	Order now
Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Have Questions?	Leave a Message or Start an on-line Chat

Recombinant Human Kynurenine 3-monooxygenase (KMO)

Product Details

Related Products

Customer Reviews and Q&A

Target Background

×CloseMessage