Recombinant Human Lysosomal-trafficking regulator (LYST), partial

Product Details

Purity

≥85% (SDS-PAGE)

Target Names

LYST

Uniprot No.

Alternative Names

Beige homolog; beige protein; Chediak-Higashi syndrome 1; CHS; CHS1; Lysosomal-trafficking regulator; LYST; LYST_HUMAN; OTTHUMP00000038327

Species

Homo sapiens (Human)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Adapter protein that regulates and/or fission of intracellular vesicles such as lysosomes. Might regulate trafficking of effectors involved in exocytosis. In cytotoxic T-cells and natural killer (NK) cells, has role in the regulation of size, number and exocytosis of lytic granules. In macrophages and dendritic cells, regulates phagosome maturation by controlling the conversion of early phagosomal compartments into late phagosomes. In macrophages and dendritic cells, specifically involved in TLR3- and TLR4-induced production of pro-inflammatory cytokines by regulating the endosomal TLR3- TICAM1/TRIF and TLR4- TICAM1/TRIF signaling pathways.

Gene References into Functions

homozygous c.6077_6078insA (p.Tyr2026Terfs) mutation was detected in the LYST gene in both patients PMID: 29652989
LYST mutations in sporadic chordoma PMID: 29026114
Pathway analysis of the genes associated with the 46 CpG sites revealed an enrichment of immune system process genes, including LYST (cg16962115, FDR = 1.24E-04), CADM1 (cg21933078, FDR = 1.22E-02) and NFATC1 (cg06784563, FDR = 1.46E-02) PMID: 28637314
Mutation in LYST gene is associated with hemophagocytic lymphohistiocytosis. PMID: 27781387
Effects of LYST mutations in Chediak-Higashi syndrome show that LYST is involved in regulation of natural killer cell lytic activity, from lytic granule size to polarization and exocytosis, as well as endolysosomal compartment identity. PMID: 26478006
Lack of LYST during endolysosomal biogenesis leads to the formation of enlarged hybrid organelles and blocks the terminal maturation of lytic granules into secretory granules. PMID: 25425525
Lysosome degradation and traffcking of cargo via autophagy, endocytosis or retrograde transport are not affected by LYST depletion. PMID: 25216107
involved in the regulation of phospholipase D activity PMID: 24830864
present an AR-HSP family with cerebellar ataxia and neuropathy with a novel homozygous missense mutation in the lysosomal trafficking regulator (LYST) gene. LYST is known as the causative gene for Chediak-Higashi syndrome. PMID: 24521565
this is the first report of a severe early-onset Chediak-Higashi syndrome (CHS0 with a homozygous LYST/CHS1 missense mutation. PMID: 24112114
gene sequencing of all exons of the lysosome trafficking regulator (CHS1/LYST) gene and revealed a nonsense mutation in exon 5 (c.925C>T, p.R309X). PMID: 24072239
R1836X mutation in the LYST gene is associated with Chediak Higashi syndrome. PMID: 21488161
Childhood, adolescent, and adult forms of Chediak-Higashi syndrome correlate with different genotypes. PMID: 11857544
The Chediak-Higashi protein interacts with SNARE complex and signal transduction proteins. PMID: 11984006
results suggest that the Beige protein interacts with at least two different partners and that the Beige protein affects cellular events, such as nuclear PtdIns(4,5)P2 localization, in addition to lysosome size PMID: 12753649
two newly described nonsense mutations are expected to give rise to a severe phenotype and the patient had absolutely no cytotoxicity by natural killer cells or cytotoxic lymphocytes PMID: 15896657
CHS1/LYST protein is involved in either vesicle fusion or fission. CHS1/LYST affects hematologic, immunologic and neurologic processes. Review. PMID: 18043242

Hide All

Involvement in disease

Chediak-Higashi syndrome (CHS)

Subcellular Location

Cytoplasm.

Tissue Specificity

Abundantly expressed in adult and fetal thymus, peripheral blood leukocytes, bone marrow and several regions of the adult brain.

Database Links

HGNC: 1968

OMIM: 214500

KEGG: hsa:1130

STRING: 9606.ENSP00000374443

UniGene: Hs.532411

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Recombinant Human Lysosomal-trafficking regulator (LYST), partial

Product Details

Customer Reviews and Q&A

Target Background

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