Recombinant Human Melanoma-associated antigen F1 (MAGEF1)

Code CSB-YP881012HU
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP881012HU
MSDS
Size Pls inquire
Source E.coli
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Code CSB-EP881012HU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP881012HU
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP881012HU
MSDS
Size Pls inquire
Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
MAGEF1
Uniprot No.
Alternative Names
MAGE F1 antigen; MAGE-F1 antigen; MAGEF 1; MAGEF1; MAGF1_HUMAN; Melanoma antigen family F; 1; Melanoma associated antigen F1; Melanoma-associated antigen F1; MGC19617
Species
Homo sapiens (Human)
Expression Region
1-307
Target Protein Sequence
MLQTPESRGL PVPQAEGEKD GGHDGETRAP TASQERPKEE LGAGREEGAA EPALTRKGAR ALAAKALARR RAYRRLNRTV AELVQFLLVK DKKKSPITRS EMVKYVIGDL KILFPDIIAR AAEHLRYVFG FELKQFDRKH HTYILINKLK PLEEEEEEDL GGDGPRLGLL MMILGLIYMR GNSAREAQVW EMLRRLGVQP SKYHFLFGYP KRLIMEDFVQ QRYLSYRRVP HTNPPEYEFS WGPRSNLEIS KMEVLGFVAK LHKKEPQHWP VQYREALADE ADRARAKARA EASMRARASA RAGIHLW
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Enhances ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin ligases. Proposed to act through recruitment and/or stabilization of the E2 ubiquitin-conjugating enzyme at the E3:substrate complex. MAGEF1-NSMCE1 ubiquitin ligase complex promotes proteasomal degradation of MMS19, a key component of the cytosolic iron-sulfur protein assembly (CIA) machinery. Down-regulation of MMS19 impairs the activity of several DNA repair and metabolism enzymes such as ERCC2/XPD, FANCJ, RTEL1 and POLD1 that require iron-sulfur clusters as cofactors. May negatively regulate genome integrity by inhibiting homologous recombination-mediated double-strand break DNA repair.
Gene References into Functions
  1. MAGE-F1 specifies the cytosolic iron-sulfur assembly pathway protein MMS19 for ubiquitination and degradation. PMID: 29225034
Tissue Specificity
Ubiquitous.
Database Links

HGNC: 29639

OMIM: 609267

KEGG: hsa:64110

STRING: 9606.ENSP00000315064

UniGene: Hs.306123

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